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学科主题: 临床医学
题名:
Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis
作者: Zhang, Wei2; Li, Tianrun3; Shao, Yong1,4; Zhang, Chao4; Wu, Qi4; Yang, Hong5; Zhang, Jie1; Guan, Ming6; Yu, Bo1,4; Wan, Jun4,7
关键词: GADD45G ; Methylation ; HRM ; Gastric cancer ; Colorectal cancer ; Pancreatic cancer
刊名: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
发表日期: 2010-08-01
DOI: 10.1007/s00432-010-0777-z
卷: 136, 期:8, 页:1267-1273
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: HUMAN PITUITARY-ADENOMAS ; NEGATIVE GROWTH-CONTROL ; CPG ISLAND METHYLATION ; CELL-CYCLE CHECKPOINTS ; DNA METHYLATION ; GENOMIC INSTABILITY ; INHIBITORY GENE ; MYD GENES ; PCR BIAS ; EXPRESSION
英文摘要:

Purpose GADD45 is a family of proteins involved in DNA damage response and cell growth arrest. GADD45G was identified as an interleukin-2-induced immediate-early gene, and methylation of GADD45G was studied in various tumor cell lines and a few primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation.

Methods In our study, we used HRM analysis to detect the methylation levels of GADD45G gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers and equal number of adjacent normal tissues.

Results The frequency of GADD45G methylation in all three types of cancers was significantly higher than that in normal tissues. Consistent with previous reports, expression levels of GADD45G were inversely correlated with methylation levels. But we did not find significant association between GADD45G methylation status and TNM staging in all three types of cancers.

Conclusions In summary, application of HRM analysis to large amount of clinical samples proves to be a fast and high-throughput way to investigate the epigenetic status of GADD45G. And this is the first study to evaluate the prevalence of GADD45G methylation based on large amount of tumor samples, showing that epigenetic regulation of GADD45G was associated with carcinogenesis.

语种: 英语
WOS记录号: WOS:000279824500017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62756
Appears in Collections:北京大学深圳医院_皮肤性病科_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen, Guangdong, Peoples R China
2.Jinan Univ, JNU HKUST Joint Lab, Guangzhou, Guangdong, Peoples R China
3.Peking Univ, Dept Intervent & Vasc Surg, Hosp 3, Beijing 100871, Peoples R China
4.Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen, Guangdong, Peoples R China
6.Fudan Univ, Cent Lab, Dept Lab Med, Huashan Hosp, Shanghai 200433, Peoples R China
7.Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Zhang, Wei,Li, Tianrun,Shao, Yong,et al. Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2010,136(8):1267-1273.
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