IR@PKUHSC  > 北京大学第二临床医学院  > 北京大学肝病研究所
学科主题临床医学
Deep sequencing of hepatitis B virus basal core promoter and precore mutants in HBeAg-positive chronic hepatitis B patients
Yan, Linlin1,2,3; Zhang, Henghui3; Ma, Hui3; Liu, Di4; Li, Wei4; Kang, Yulin5; Yang, Ruifeng3; Wang, Jianghua3; He, Gaixia3; Xie, Xingwang3; Wang, Hao3; Wei, Lai3; Lu, Zuhong5; Shao, Qixiang1,2; Chen, Hongsong3
刊名SCIENTIFIC REPORTS
2015-12-09
DOI10.1038/srep17950
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Natural Science Foundation of China ; National Key Sci-Tech Special Project of China ; National Science and Technology Major Project for Infectious Diseases Control during the 12th Five-Year Plan Period ; Key Project of Beijing Science and Technology Commission ; Beijing Natural Science Foundation ; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine ; Clinical Medicine Science &amp ; Technology Project of Jiangsu Province of China ; Natural Science Foundation of China ; National Key Sci-Tech Special Project of China ; National Science and Technology Major Project for Infectious Diseases Control during the 12th Five-Year Plan Period ; Key Project of Beijing Science and Technology Commission ; Beijing Natural Science Foundation ; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine ; Clinical Medicine Science &amp ; Technology Project of Jiangsu Province of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]E-ANTIGEN SEROCONVERSION ; HEPATOCELLULAR-CARCINOMA ; VIRAL REPLICATION ; LIVER-DAMAGE ; MUTATIONS ; CARRIERS ; RISK ; ASSOCIATION ; INFECTION ; GENOTYPES
英文摘要

Mutants in the basal core promoter (BCP) and precore (PC) regions of hepatitis B virus (HBV) genome are associated with the progression of chronic hepatitis B (CHB) infection. However, quasispecies characteristics of naturally occurring mutants in those regions in HBeAg-positive CHB patients has not been well described, partly limited by quantitative assay. This study aimed to develop an Ion Torrent deep sequencing assay to determine BCP and PC mutant percentages in HBeAg-positive CHB patients who were treatment naive and correlate them with different viral and host factors. Our results showed that Ion Torrent deep sequencing could achieve high accuracy (R-2>0.99) within a dynamic range between 1% and 100%. Twelve hotspots with prevalence of greater than 20% were observed in EnhII/BCP/PC regions. G1719T, T1753V, A1762T and G1764A were genotype C related. BCP A1762T/G1764A double mutants were generally accompanied with PC 1896 wild type or lower PC G1896A mutant percentage. Lower serum HBeAg and HBsAg levels were associated with higher BCP A1762T/G1764A mutant percentages (>= 50%). ALT levels were higher in patients with PC G1896A mutant percentage greater than 10%. In conclusion, deep sequencing such as Ion Torrent sequencing could accurately quantify HBV mutants for providing clinical relevant information during HBV infection.

语种英语
所属项目编号81273202 ; 31400773 ; 81201339 ; 2012ZX10002011-006 ; 2012ZX10002003 ; D121100003912003 ; 7152151 ; JSKLM-2014-008 ; BL2013024
资助者Natural Science Foundation of China ; National Key Sci-Tech Special Project of China ; National Science and Technology Major Project for Infectious Diseases Control during the 12th Five-Year Plan Period ; Key Project of Beijing Science and Technology Commission ; Beijing Natural Science Foundation ; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine ; Clinical Medicine Science &amp ; Technology Project of Jiangsu Province of China ; Natural Science Foundation of China ; National Key Sci-Tech Special Project of China ; National Science and Technology Major Project for Infectious Diseases Control during the 12th Five-Year Plan Period ; Key Project of Beijing Science and Technology Commission ; Beijing Natural Science Foundation ; Jiangsu Key Laboratory of Medical Science and Laboratory Medicine ; Clinical Medicine Science &amp ; Technology Project of Jiangsu Province of China
WOS记录号WOS:000366038400001
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62765
Collection北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Jiangsu Univ, Sch Med, Dept Immunol, Zhenjiang 212013, Jiangsu, Peoples R China
2.Jiangsu Univ, Sch Med, Jiangsu Key Lab Med Sci & Lab Med, Zhenjiang 212013, Jiangsu, Peoples R China
3.Peking Univ, Inst Hepatol, Peoples Hosp, Beijing Key Lab Hepatitis & Immunotherapy Liver C, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China
5.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Yan, Linlin,Zhang, Henghui,Ma, Hui,et al. Deep sequencing of hepatitis B virus basal core promoter and precore mutants in HBeAg-positive chronic hepatitis B patients[J]. SCIENTIFIC REPORTS,2015,5.
APA Yan, Linlin.,Zhang, Henghui.,Ma, Hui.,Liu, Di.,Li, Wei.,...&Chen, Hongsong.(2015).Deep sequencing of hepatitis B virus basal core promoter and precore mutants in HBeAg-positive chronic hepatitis B patients.SCIENTIFIC REPORTS,5.
MLA Yan, Linlin,et al."Deep sequencing of hepatitis B virus basal core promoter and precore mutants in HBeAg-positive chronic hepatitis B patients".SCIENTIFIC REPORTS 5(2015).
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