IR@PKUHSC  > 北京大学药学院  > 天然药物与仿生药物国家重点实验室
学科主题药学
Preparation and application of novel microspheres possessing autofluorescent properties
Wei, Wei; Wang, Lian-Yan; Yuan, Lan; Wei, Qiang; Yang, Xiao-Da; Su, Zhi-Guo; Ma, Guang-Hui
刊名ADVANCED FUNCTIONAL MATERIALS
2007-11-05
DOI10.1002/adfm.200700274
17期:16页:3153-3158
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
研究领域[WOS]Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]FLUORESCENT LATEX MICROSPHERES ; BLOOD-FLOW DISTRIBUTION ; NANOPARTICLES ; NANOSPHERES
英文摘要

Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The auto-fluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers.

语种英语
WOS记录号WOS:000250989600017
Citation statistics
Cited Times:99[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62769
Collection北京大学药学院_天然药物与仿生药物国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China
4.Peking Univ, Hlth Sci Ctr, State Key Labs Nat & Biomimet Drugs, Dept Biol Chem, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Wei, Wei,Wang, Lian-Yan,Yuan, Lan,et al. Preparation and application of novel microspheres possessing autofluorescent properties[J]. ADVANCED FUNCTIONAL MATERIALS,2007,17(16):3153-3158.
APA Wei, Wei.,Wang, Lian-Yan.,Yuan, Lan.,Wei, Qiang.,Yang, Xiao-Da.,...&Ma, Guang-Hui.(2007).Preparation and application of novel microspheres possessing autofluorescent properties.ADVANCED FUNCTIONAL MATERIALS,17(16),3153-3158.
MLA Wei, Wei,et al."Preparation and application of novel microspheres possessing autofluorescent properties".ADVANCED FUNCTIONAL MATERIALS 17.16(2007):3153-3158.
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