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Adrenomedullin and adrenotensin increase the transcription of regulator of G-protein signaling 2 in vascular smooth muscle cells via the cAMP-dependent and PKC pathways
Mao, Yuanjie1,2; Su, Jialin1,3,4; Lei, Lei5; Meng, Lei1; Qi, Yongfen1,6; Huo, Yong1; Tang, Chaoshu1,6
关键词adrenomedullin adrenotensin regulator of G-protein signaling 2 vascular smooth muscle cells
刊名MOLECULAR MEDICINE REPORTS
2014
DOI10.3892/mmr.2013.1751
9期:1页:323-327
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]NATRIURETIC-PEPTIDE ; GENERAL-POPULATION ; CONSCIOUS SHEEP ; RAT ; RESPONSES ; RGS2 ; PROADRENOMEDULLIN ; POLYMORPHISMS ; EXPRESSION ; PRODUCT
英文摘要

The regulator of G-protein signaling 2 (RGS2) has been shown to be crucial in the regulation of vascular tone and blood pressure. The vascular activities of adrenomedullin (ADM) and adrenotension (ADT), two natural peptides, are dependent upon the modulation of RGS2 expression. However, the effects and pathways involved in their modulation remain unknown. This study aimed to observe the changes of RGS2 expression in response to ADM and ADT in cultured vascular smooth muscle cells and to clarify the potential signaling pathways in vitro. In the present study, vascular smooth muscle cells (VSMCs) were cultured with ADM and ADT of various concentrations for different time periods, and the gene expression of RGS2 was analyzed by PCR. ADM significantly increased the gene expression at 0.5 h to similar to 35-fold of that at baseline, whereas ADT marginally increased the expression after 1-2 h. SQ22,536 and chelerythrine were used to block the protein kinase A (PKA) and PKC pathways activated by incubation with ADM. The gene expression of RGS2 was reduced by SQ22,536 only. Furthermore, when SQ22,536 and chelerythrine were added to the cells incubated with ADT, the gene expression was markedly reduced by both SQ22,536 and chelerythrine. In conclusion, ADM immediately showed a marked increase in the gene expression of RGS2 in cultured VSMCs via a cAMP-dependent pathway and ADT gradually showed a marginal increase in the gene expression via a cAMP-dependent pathway and a PKC pathway.

语种英语
WOS记录号WOS:000330783700051
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62772
专题北京大学第一临床医学院_心血管内科
作者单位1.Brown Univ, Warren Alpert Med Sch, Providence, RI 02912 USA
2.Peking Univ, Dept Cardiol, Hosp 1, Beijing 100034, Peoples R China
3.Natl Cerebral & Cardiovasc Ctr, Dept Biochem, Suita, Osaka 5658565, Japan
4.Brown Univ, Cardiovasc Res Ctr, Rhode Isl Hosp, Cardiol Div, Providence, RI 02912 USA
5.Beijing Aerosp Ctr Hosp, Dept Endocrinol, Beijing 100049, Peoples R China
6.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100083, Peoples R China
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Mao, Yuanjie,Su, Jialin,Lei, Lei,et al. Adrenomedullin and adrenotensin increase the transcription of regulator of G-protein signaling 2 in vascular smooth muscle cells via the cAMP-dependent and PKC pathways[J]. MOLECULAR MEDICINE REPORTS,2014,9(1):323-327.
APA Mao, Yuanjie.,Su, Jialin.,Lei, Lei.,Meng, Lei.,Qi, Yongfen.,...&Tang, Chaoshu.(2014).Adrenomedullin and adrenotensin increase the transcription of regulator of G-protein signaling 2 in vascular smooth muscle cells via the cAMP-dependent and PKC pathways.MOLECULAR MEDICINE REPORTS,9(1),323-327.
MLA Mao, Yuanjie,et al."Adrenomedullin and adrenotensin increase the transcription of regulator of G-protein signaling 2 in vascular smooth muscle cells via the cAMP-dependent and PKC pathways".MOLECULAR MEDICINE REPORTS 9.1(2014):323-327.
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