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学科主题: 临床医学
题名:
Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
作者: Xu, Ling1,2; Hausmann, Martin1,3; Dietmaier, Wolfgang4; Kellermeier, Silvia1,3; Pesch, Theresa3; Stieber-Gunckel, Manuela1; Lippert, Elisabeth1; Klebl, Frank1; Rogler, Gerhard1,3
刊名: BMC CANCER
发表日期: 2010-06-18
DOI: 10.1186/1471-2407-10-302
卷: 10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: UP-REGULATES P27(KIP1) ; MONOCLONAL-ANTIBODY ; CHOLANGIOCELLULAR CARCINOMA ; COLORECTAL-CANCER ; TUMOR-GROWTH ; NUDE-MICE ; INHIBITION ; CETUXIMAB ; INSULIN ; APOPTOSIS
英文摘要:

Background: Cholangiocarcinoma (CC) is a malignant neoplasm of the bile ducts or the gallbladder. Targeting of growth factor receptors showed therapeutic potential in palliative settings for many solid tumors. The aim of this study was to determine the expression of seven growth factor receptors in CC cell lines and to assess the effect of blocking the EGFR receptor in vitro.

Methods: Expression of EGFR (epithelial growth factor receptor), HGFR (hepatocyte growth factor receptor) IGF1R (insulin-like growth factor 1 receptor), IGF2R (insulin-like growth factor 2 receptor) and VEGFR1-3 (vascular endothelial growth factor receptor 1-3) were examined in four human CC cell lines (EGI-1, HuH28, OZ and TFK-1). The effect of the anti-EGFR-antibody cetuximab on cell growth and apoptosis was studied and cell lines were examined for KRAS mutations.

Results: EGFR, HGFR and IGFR1 were present in all four cell lines tested. IGFR2 expression was confirmed in EGI-1 and TFK-1. No growth-inhibitory effect was found in EGI-1 cells after incubation with cetuximab. Cetuximab dose-dependently inhibited growth in TFK-1. Increased apoptosis was only seen in TFK-1 cells at the highest cetuximab dose tested (1 mg/ml), with no dose-response-relationship at lower concentrations. In EGI-1 a heterozygous KRAS mutation was found in codon 12 (c.35G>A; p.G12D). HuH28, OZ and TFK-1 lacked KRAS mutation.

Conclusion: CC cell lines express a pattern of different growth receptors in vitro. Growth factor inhibitor treatment could be affected from the KRAS genotype in CC. The expression of EGFR itself does not allow prognoses on growth inhibition by cetuximab.

语种: 英语
WOS记录号: WOS:000279796700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62775
Appears in Collections:北京大学第一临床医学院_肿瘤化疗科_期刊论文

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作者单位: 1.Univ Regensburg, Dept Pathol, D-93042 Regensburg, Germany
2.Univ Regensburg, Dept Internal Med 1, D-93042 Regensburg, Germany
3.Peking Univ, Hosp 1, Dept Med Oncol, Beijing 100034, Peoples R China
4.Univ Zurich Hosp, Dept Internal Med, Clin Gastroenterol & Hepatol, CH-8091 Zurich, Switzerland

Recommended Citation:
Xu, Ling,Hausmann, Martin,Dietmaier, Wolfgang,et al. Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines[J]. BMC CANCER,2010,10.
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