IR@PKUHSC  > 北京大学第一临床医学院  > 肿瘤化疗科
学科主题临床医学
Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
Xu, Ling1,2; Hausmann, Martin1,3; Dietmaier, Wolfgang4; Kellermeier, Silvia1,3; Pesch, Theresa3; Stieber-Gunckel, Manuela1; Lippert, Elisabeth1; Klebl, Frank1; Rogler, Gerhard1,3
刊名BMC CANCER
2010-06-18
DOI10.1186/1471-2407-10-302
10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]UP-REGULATES P27(KIP1) ; MONOCLONAL-ANTIBODY ; CHOLANGIOCELLULAR CARCINOMA ; COLORECTAL-CANCER ; TUMOR-GROWTH ; NUDE-MICE ; INHIBITION ; CETUXIMAB ; INSULIN ; APOPTOSIS
英文摘要

Background: Cholangiocarcinoma (CC) is a malignant neoplasm of the bile ducts or the gallbladder. Targeting of growth factor receptors showed therapeutic potential in palliative settings for many solid tumors. The aim of this study was to determine the expression of seven growth factor receptors in CC cell lines and to assess the effect of blocking the EGFR receptor in vitro.

Methods: Expression of EGFR (epithelial growth factor receptor), HGFR (hepatocyte growth factor receptor) IGF1R (insulin-like growth factor 1 receptor), IGF2R (insulin-like growth factor 2 receptor) and VEGFR1-3 (vascular endothelial growth factor receptor 1-3) were examined in four human CC cell lines (EGI-1, HuH28, OZ and TFK-1). The effect of the anti-EGFR-antibody cetuximab on cell growth and apoptosis was studied and cell lines were examined for KRAS mutations.

Results: EGFR, HGFR and IGFR1 were present in all four cell lines tested. IGFR2 expression was confirmed in EGI-1 and TFK-1. No growth-inhibitory effect was found in EGI-1 cells after incubation with cetuximab. Cetuximab dose-dependently inhibited growth in TFK-1. Increased apoptosis was only seen in TFK-1 cells at the highest cetuximab dose tested (1 mg/ml), with no dose-response-relationship at lower concentrations. In EGI-1 a heterozygous KRAS mutation was found in codon 12 (c.35G>A; p.G12D). HuH28, OZ and TFK-1 lacked KRAS mutation.

Conclusion: CC cell lines express a pattern of different growth receptors in vitro. Growth factor inhibitor treatment could be affected from the KRAS genotype in CC. The expression of EGFR itself does not allow prognoses on growth inhibition by cetuximab.

语种英语
WOS记录号WOS:000279796700001
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62775
专题北京大学第一临床医学院_肿瘤化疗科
作者单位1.Univ Regensburg, Dept Pathol, D-93042 Regensburg, Germany
2.Univ Regensburg, Dept Internal Med 1, D-93042 Regensburg, Germany
3.Peking Univ, Hosp 1, Dept Med Oncol, Beijing 100034, Peoples R China
4.Univ Zurich Hosp, Dept Internal Med, Clin Gastroenterol & Hepatol, CH-8091 Zurich, Switzerland
推荐引用方式
GB/T 7714
Xu, Ling,Hausmann, Martin,Dietmaier, Wolfgang,et al. Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines[J]. BMC CANCER,2010,10.
APA Xu, Ling.,Hausmann, Martin.,Dietmaier, Wolfgang.,Kellermeier, Silvia.,Pesch, Theresa.,...&Rogler, Gerhard.(2010).Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines.BMC CANCER,10.
MLA Xu, Ling,et al."Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines".BMC CANCER 10(2010).
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