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Cloning, expression, and characterization of the beta-glucosidase hydrolyzing secoisolariciresinol diglucoside to secoisolariciresinol from Bacteroides uniformis ZL1
Tao, Y. -L.1; Yang, D. -H.1; Zhang, Y. -T.1; Zhang, Y.1; Wang, Z. -Q.2; Wang, Y. -S.2; Cai, S. -Q.1; Liu, S. -L.3,4
关键词Bacteroides uniformis beta-glucosidase Secoisolariciresinol diglucoside Secoisolariciresinol Recombinant bacteria
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
2014-03-01
DOI10.1007/s00253-013-5111-7
98期:6页:2519-2531
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]HUMAN INTESTINAL BACTERIA ; ESCHERICHIA-COLI ; PROTEIN-PRODUCTION ; SEQUENCE-ANALYSIS ; PURIFICATION ; ENTERODIOL ; ENTEROLACTONE ; STRATEGIES ; BIOTRANSFORMATION ; IMPROVEMENT
英文摘要

Previously, from the human intestinal flora we isolated the bacterial strain Bacteroides uniformis ZL1, which could convert secoisolariciresinol diglucoside (SDG) to its aglycone secoisolariciresinol (SECO) in vivo. In this study, 24 putative beta-glucosidase genes were screened from the genome of B. uniformis ATCC 8492, which were used as templates to design PCR primers for the target genes in B. uniformis ZL1. Fifteen genes (bgl1-bgl15) were amplified from strain ZL1, and among them we identified bgl8 as the gene encoding the SDG-hydrolyzing beta-glucosidase. We sequenced the bgl8 gene, cloned it into the expression vector and then transformed Escherichia coli to construct the recombinant bacteria that could synthesize the target beta-glucosidase (BuBGL8). We purified and characterized BuBGL8, which showed maximal activity and stability under the culture conditions of pH 6.0 and 30 A degrees C. SDG (2.0 mg/ml) was converted to SECO by both the purified BuBGL8 (0.035 mg/ml) and crude enzyme extract (0.23 mg crude protein/ml) with the efficiency of more than 90 % after 90 min at the reaction conditions. This is, to our knowledge, the first report of using recombinant bacteria to synthesize the SDG-hydrolyzing beta-glucosidase, which could be used to produce SECO from SDG conveniently and highly efficiently.

语种英语
WOS记录号WOS:000332108100014
项目编号21072012 ; 2011ZX09102-011-06 ; 20100001110059
资助机构National Natural Science Foundation of China ; National Science and Technology Major Projects for Major New Drugs Innovation and Development, China ; Doctoral Fund of Ministry of Education of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62778
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Harbin Med Univ, Genom Res Ctr, Harbin 150081, Peoples R China
4.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada
推荐引用方式
GB/T 7714
Tao, Y. -L.,Yang, D. -H.,Zhang, Y. -T.,et al. Cloning, expression, and characterization of the beta-glucosidase hydrolyzing secoisolariciresinol diglucoside to secoisolariciresinol from Bacteroides uniformis ZL1[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(6):2519-2531.
APA Tao, Y. -L..,Yang, D. -H..,Zhang, Y. -T..,Zhang, Y..,Wang, Z. -Q..,...&Liu, S. -L..(2014).Cloning, expression, and characterization of the beta-glucosidase hydrolyzing secoisolariciresinol diglucoside to secoisolariciresinol from Bacteroides uniformis ZL1.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(6),2519-2531.
MLA Tao, Y. -L.,et al."Cloning, expression, and characterization of the beta-glucosidase hydrolyzing secoisolariciresinol diglucoside to secoisolariciresinol from Bacteroides uniformis ZL1".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.6(2014):2519-2531.
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