IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Rat CC chemokine receptor 4 is the functional homologue of human CC chemokine receptor 4 and can interact with human CCL17 and CCL22
Tian LinJie1,2; Qi Hui1,2; Xie Yuan1,2; Zhang YingMei1,2; Zhang WenJuan1,2; Sun XiangYu1,2; Wang Ying1,2; Ma DaLong1,2
关键词CCL17 CCL22 CCR4 rat cross-species interaction
刊名CHINESE SCIENCE BULLETIN
2010-05-01
DOI10.1007/s11434-010-0157-8
55期:14页:1388-1395
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ACTIVATION-REGULATED CHEMOKINE ; MACROPHAGE-DERIVED CHEMOKINE ; CD4(+) T-CELLS ; ATOPIC-DERMATITIS ; MOLECULAR-CLONING ; DENDRITIC CELLS ; CC-CHEMOKINE-RECEPTOR-4 CCR4 ; BRONCHOALVEOLAR LAVAGE ; DISEASE-ACTIVITY ; MESSENGER-RNA
英文摘要

Human CCL17/TARC (hCCL17) and CCL22/MDC (hCCL22) interact with their receptor CCR4, playing pivotal roles in various Th2 cell-dominant diseases. Rat Ccl17, Ccl22 and Ccr4 share 63.4%, 65.2% and 87.5% homology at the amino acid level, respectively, compared with their human homologues. Rat Ccl22 has been demonstrated to interact with rat Ccr4. However, it is not known whether rat Ccl17 is a functional ligand for rat Ccr4 and whether rat Ccr4 can interact with hCCL17 and hCCL22. In this study, we cloned rat Ccl17 and Ccr4 cDNA from rat thymus and expressed the recombinant rat Ccl17. The binding assay indicated rat Ccl17 could saturably bind rat Ccr4, and this binding could be competitively inhibited by unlabeled rat Cc17, hCCL17 or hCCL22. Both rat and human CCL17 or CCL22 could induce the chemotactic migration and calcium mobilization in rat Ccr4-transfected cells. They could also desensitize each other′s effect on chemotaxis and calcium mobilization. Furthermore, both rat Ccl17 and hCCL17 could induce significant receptor internalization in rat Ccr4-EGFP transfected cells. These results demonstrated the conserved CCL17/CCR4 interaction in rats and the further cross-species interaction of rat Ccr4 with hCCL17 and hCCL22. Likewise, they provided the molecular basis for the investigation of physiological and pharmacological roles of CCR4, its ligands, and their antagonists in rat disease models.

语种英语
WOS记录号WOS:000277335300008
项目编号2006AA02A305 ; 30671907 ; 30872292 ; 90813025 ; 5072029
资助机构National High-Tech Research and Development Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62779
专题北京大学基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100191, Peoples R China
2.Peking Univ, Human Dis Genom Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Tian LinJie,Qi Hui,Xie Yuan,et al. Rat CC chemokine receptor 4 is the functional homologue of human CC chemokine receptor 4 and can interact with human CCL17 and CCL22[J]. CHINESE SCIENCE BULLETIN,2010,55(14):1388-1395.
APA Tian LinJie.,Qi Hui.,Xie Yuan.,Zhang YingMei.,Zhang WenJuan.,...&Ma DaLong.(2010).Rat CC chemokine receptor 4 is the functional homologue of human CC chemokine receptor 4 and can interact with human CCL17 and CCL22.CHINESE SCIENCE BULLETIN,55(14),1388-1395.
MLA Tian LinJie,et al."Rat CC chemokine receptor 4 is the functional homologue of human CC chemokine receptor 4 and can interact with human CCL17 and CCL22".CHINESE SCIENCE BULLETIN 55.14(2010):1388-1395.
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