北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Identification of a novel testicular orphan receptor-4 (TR4)-associated protein as repressor for the selective suppression of TR4-mediated Transactivation
作者: Yang, Y; Wang, X; Dong, TF; Kim, E; Lin, WJ; Chang, CS
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2003-02-28
DOI: 10.1074/jbc.M207116200
卷: 278, 期:9, 页:7709-7717
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: NUCLEAR HORMONE RECEPTORS ; COREPRESSOR N-COR ; STEROID-RECEPTOR ; THYROID-HORMONE ; HISTONE DEACETYLASE ; ANDROGEN RECEPTOR ; TRANSCRIPTIONAL REPRESSION ; GENE-EXPRESSION ; SIGNALING PATHWAYS ; MOLECULAR-CLONING
英文摘要:

Although many co-activators have been identified for various nuclear receptors, relatively fewer co-repressors have been isolated and characterized. Here we report the identification of a novel testicular orphan nuclear receptor-4 (TR4)-associated protein (TRA16) that is mainly localized in the nucleus of cells as a repressor to suppress TR4-mediated transactivation. The suppression of TR4-mediated transactivation is selective because TRA16 shows only a slight influence on the transactivation of androgen receptor, glucocorticoid receptor, and progesterone receptor. Sequence analysis shows that TRA16 is a novel gene with 139 amino acids in an open reading frame with a molecular mass of 16 kDa, which did not match any published gene sequences. Mammalian two-hybrid system and co-immunoprecipitation assays both demonstrate that TRA16 can interact strongly with TR4. The electrophoretic mobility shift assay suggests that TRA16 may suppress TR4-mediated transactivation via decreased binding between the TR4 protein and the TR4 response element on the target gene(s). Furthermore, TRA16 can also block the interaction between TR4 and TR4 ligand-binding domain through interacting with TR4-DNA-binding and ligand-binding domains. These unique suppression mechanisms suggest that TRA16 may function as a novel repressor to selectively suppress the TR4-mediated transactivation.

语种: 英语
WOS记录号: WOS:000181195100136
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62782
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Rochester, Ctr Canc, Rochester, NY 14642 USA
2.Peking Univ, Hosp 1, Dept Surg, Beijing 100034, Peoples R China
3.Univ Rochester, George Whipple Lab Canc Res, Dept Pathol, Rochester, NY 14642 USA
4.Univ Rochester, George Whipple Lab Canc Res, Dept Urol, Rochester, NY 14642 USA
5.Univ Rochester, George Whipple Lab Canc Res, Dept Radiat Oncol, Rochester, NY 14642 USA
6.Peking Univ, Beijing Canc Hosp, Dept Surg, Beijing Inst Canc Res, Beijing 100036, Peoples R China

Recommended Citation:
Yang, Y,Wang, X,Dong, TF,et al. Identification of a novel testicular orphan receptor-4 (TR4)-associated protein as repressor for the selective suppression of TR4-mediated Transactivation[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2003,278(9):7709-7717.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yang, Y]'s Articles
[Wang, X]'s Articles
[Dong, TF]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yang, Y]‘s Articles
[Wang, X]‘s Articles
[Dong, TF]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace