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学科主题临床医学
Identification of a novel testicular orphan receptor-4 (TR4)-associated protein as repressor for the selective suppression of TR4-mediated Transactivation
Yang, Y; Wang, X; Dong, TF; Kim, E; Lin, WJ; Chang, CS
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2003-02-28
DOI10.1074/jbc.M207116200
278期:9页:7709-7717
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]NUCLEAR HORMONE RECEPTORS ; COREPRESSOR N-COR ; STEROID-RECEPTOR ; THYROID-HORMONE ; HISTONE DEACETYLASE ; ANDROGEN RECEPTOR ; TRANSCRIPTIONAL REPRESSION ; GENE-EXPRESSION ; SIGNALING PATHWAYS ; MOLECULAR-CLONING
英文摘要

Although many co-activators have been identified for various nuclear receptors, relatively fewer co-repressors have been isolated and characterized. Here we report the identification of a novel testicular orphan nuclear receptor-4 (TR4)-associated protein (TRA16) that is mainly localized in the nucleus of cells as a repressor to suppress TR4-mediated transactivation. The suppression of TR4-mediated transactivation is selective because TRA16 shows only a slight influence on the transactivation of androgen receptor, glucocorticoid receptor, and progesterone receptor. Sequence analysis shows that TRA16 is a novel gene with 139 amino acids in an open reading frame with a molecular mass of 16 kDa, which did not match any published gene sequences. Mammalian two-hybrid system and co-immunoprecipitation assays both demonstrate that TRA16 can interact strongly with TR4. The electrophoretic mobility shift assay suggests that TRA16 may suppress TR4-mediated transactivation via decreased binding between the TR4 protein and the TR4 response element on the target gene(s). Furthermore, TRA16 can also block the interaction between TR4 and TR4 ligand-binding domain through interacting with TR4-DNA-binding and ligand-binding domains. These unique suppression mechanisms suggest that TRA16 may function as a novel repressor to selectively suppress the TR4-mediated transactivation.

语种英语
WOS记录号WOS:000181195100136
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62782
专题北京大学临床肿瘤学院
作者单位1.Univ Rochester, Ctr Canc, Rochester, NY 14642 USA
2.Peking Univ, Hosp 1, Dept Surg, Beijing 100034, Peoples R China
3.Univ Rochester, George Whipple Lab Canc Res, Dept Pathol, Rochester, NY 14642 USA
4.Univ Rochester, George Whipple Lab Canc Res, Dept Urol, Rochester, NY 14642 USA
5.Univ Rochester, George Whipple Lab Canc Res, Dept Radiat Oncol, Rochester, NY 14642 USA
6.Peking Univ, Beijing Canc Hosp, Dept Surg, Beijing Inst Canc Res, Beijing 100036, Peoples R China
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Yang, Y,Wang, X,Dong, TF,et al. Identification of a novel testicular orphan receptor-4 (TR4)-associated protein as repressor for the selective suppression of TR4-mediated Transactivation[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2003,278(9):7709-7717.
APA Yang, Y,Wang, X,Dong, TF,Kim, E,Lin, WJ,&Chang, CS.(2003).Identification of a novel testicular orphan receptor-4 (TR4)-associated protein as repressor for the selective suppression of TR4-mediated Transactivation.JOURNAL OF BIOLOGICAL CHEMISTRY,278(9),7709-7717.
MLA Yang, Y,et al."Identification of a novel testicular orphan receptor-4 (TR4)-associated protein as repressor for the selective suppression of TR4-mediated Transactivation".JOURNAL OF BIOLOGICAL CHEMISTRY 278.9(2003):7709-7717.
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