IR@PKUHSC  > 北京大学药学院
学科主题药学
From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats
Xiang, Cheng; Qiao, Xue; Wang, Qing; Li, Rui; Miao, Wenjuan; Guo, Dean; Ye, Min
刊名DRUG METABOLISM AND DISPOSITION
2011-09-01
DOI10.1124/dmd.111.038695
39期:9页:1597-1608
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
资助者Peking University ; Ministry of Science and Technology of China ; Peking University ; Ministry of Science and Technology of China
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]HUMAN LIVER-MICROSOMES ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; IN-VITRO ; GLYCYRRHIZA-URALENSIS ; ARRAY DETECTION ; BIOCHANIN-A ; PHARMACOKINETICS ; ISOLIQUIRITIGENIN ; IDENTIFICATION
英文摘要

Because of the complicated chemical composition of traditional Chinese medicines (TCMs), their metabolic study has been a great challenge, especially when they are used in the traditional way, i.e., clinical oral dose of water decoction. Poor understanding of metabolic pathways and too low metabolite concentrations to be detected in biosamples are the major hurdles. In the present work, a three-step strategy was proposed to systematically characterize in vivo metabolites of TCMs at a normal clinical dosage. Licorice, one of the most popular TCMs, was studied as a model. First, 10 representative compounds of licorice were administered to rats separately. A total of 68 metabolites were characterized by high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry and liquid chromatography (LC)/quadrupole time-of-flight-mass spectrometry (MS) analyses, together with enzyme hydrolysis. Among these, 13 compounds were confirmed by comparison with reference standards, including the 10 administered licorice compounds. Second, a high dose (equivalent to 20-fold clinical dosage) of licorice water extract was administered, and 22 more metabolites were characterized. Finally, these metabolites (including constituents of licorice) were determined by a highly sensitive and selective LC/selected reaction monitoring-MS method when the licorice water decoction was orally administered to rats at a clinical dosage (0.9 g crude drug/kg). A total of 42 metabolites in plasma and 62 metabolites in urine were detected. This is the first attempt to fully profile the in vivo metabolites of licorice at a normal clinical dosage.

语种英语
所属项目编号985-2-119-121 ; 2009ZX09304-002
资助者Peking University ; Ministry of Science and Technology of China ; Peking University ; Ministry of Science and Technology of China
WOS记录号WOS:000294050900015
Citation statistics
Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62785
Collection北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Xiang, Cheng,Qiao, Xue,Wang, Qing,et al. From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats[J]. DRUG METABOLISM AND DISPOSITION,2011,39(9):1597-1608.
APA Xiang, Cheng.,Qiao, Xue.,Wang, Qing.,Li, Rui.,Miao, Wenjuan.,...&Ye, Min.(2011).From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats.DRUG METABOLISM AND DISPOSITION,39(9),1597-1608.
MLA Xiang, Cheng,et al."From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats".DRUG METABOLISM AND DISPOSITION 39.9(2011):1597-1608.
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