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From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats
Xiang, Cheng; Qiao, Xue; Wang, Qing; Li, Rui; Miao, Wenjuan; Guo, Dean; Ye, Min
刊名DRUG METABOLISM AND DISPOSITION
2011-09-01
DOI10.1124/dmd.111.038695
39期:9页:1597-1608
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]HUMAN LIVER-MICROSOMES ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; IN-VITRO ; GLYCYRRHIZA-URALENSIS ; ARRAY DETECTION ; BIOCHANIN-A ; PHARMACOKINETICS ; ISOLIQUIRITIGENIN ; IDENTIFICATION
英文摘要

Because of the complicated chemical composition of traditional Chinese medicines (TCMs), their metabolic study has been a great challenge, especially when they are used in the traditional way, i.e., clinical oral dose of water decoction. Poor understanding of metabolic pathways and too low metabolite concentrations to be detected in biosamples are the major hurdles. In the present work, a three-step strategy was proposed to systematically characterize in vivo metabolites of TCMs at a normal clinical dosage. Licorice, one of the most popular TCMs, was studied as a model. First, 10 representative compounds of licorice were administered to rats separately. A total of 68 metabolites were characterized by high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry and liquid chromatography (LC)/quadrupole time-of-flight-mass spectrometry (MS) analyses, together with enzyme hydrolysis. Among these, 13 compounds were confirmed by comparison with reference standards, including the 10 administered licorice compounds. Second, a high dose (equivalent to 20-fold clinical dosage) of licorice water extract was administered, and 22 more metabolites were characterized. Finally, these metabolites (including constituents of licorice) were determined by a highly sensitive and selective LC/selected reaction monitoring-MS method when the licorice water decoction was orally administered to rats at a clinical dosage (0.9 g crude drug/kg). A total of 42 metabolites in plasma and 62 metabolites in urine were detected. This is the first attempt to fully profile the in vivo metabolites of licorice at a normal clinical dosage.

语种英语
WOS记录号WOS:000294050900015
项目编号985-2-119-121 ; 2009ZX09304-002
资助机构Peking University ; Ministry of Science and Technology of China
引用统计
被引频次:57[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62785
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学第一临床医学院_手术室
北京大学精神卫生研究所_药房
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xiang, Cheng,Qiao, Xue,Wang, Qing,et al. From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats[J]. DRUG METABOLISM AND DISPOSITION,2011,39(9):1597-1608.
APA Xiang, Cheng.,Qiao, Xue.,Wang, Qing.,Li, Rui.,Miao, Wenjuan.,...&Ye, Min.(2011).From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats.DRUG METABOLISM AND DISPOSITION,39(9),1597-1608.
MLA Xiang, Cheng,et al."From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats".DRUG METABOLISM AND DISPOSITION 39.9(2011):1597-1608.
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