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E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours
Chen, CL; Liu, SS; Ip, SM; Wong, LC; Ng, TY; Ngan, HYS
关键词E-cadherin methylation cervical cancer MSP methyltransferase antisense oligonucleotide
刊名EUROPEAN JOURNAL OF CANCER
2003-03-01
39期:4页:517-523
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]METHYLTRANSFERASE EXPRESSION ; CPG METHYLATION ; COLON-CANCER ; CYTOSINE-DNA ; CARCINOMA ; GENE ; PROGRESSION ; HYPERMETHYLATION ; COMPLEX ; CATENINS
英文摘要

A previous study showed E-cadherin expression was lost in some cervical cancer cell lines and tumours. This study was designed to clarify the significance of DNA methylation in silencing E-cadherin expression. We examined promoter methylation of E-cadherin in five cervical cancer cell lines and 20 cervical cancer tissues using methylation-specific PCR (MSP) and bisulphite DNA sequencing. The correlation of E-cadherin methylation and expression together with methyltransferase (DNMT1) were further studied. We found that hypermethylation of E-cadherin was involved in five cervical cancer cell lines and 40% (8/20) of cervical cancer tissues. E-cadherin protein was lost in 6/8 (75%) samples and 3/5 (60%) cell lines with promoter methylation. E-cadherin methylation was significantly correlated with increased DNMT1. Using an antisense DNMT1 oligo to transfect into SiHa HeLa C33A cell line, E-cadherin protein was re-expressed. We concluded that loss of E-cadherin expression was in part correlated with DNA methylation and DNMT1 expression in cervical cancer. (C) 2003 Elsevier Science Ltd. All rights reserved.

语种英语
WOS记录号WOS:000181686800026
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被引频次:68[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62786
专题北京大学第一临床医学院
作者单位1.Univ Hong Kong, Dept Obstet & Gynecol, Queen Mary Hosp, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
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GB/T 7714
Chen, CL,Liu, SS,Ip, SM,et al. E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours[J]. EUROPEAN JOURNAL OF CANCER,2003,39(4):517-523.
APA Chen, CL,Liu, SS,Ip, SM,Wong, LC,Ng, TY,&Ngan, HYS.(2003).E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours.EUROPEAN JOURNAL OF CANCER,39(4),517-523.
MLA Chen, CL,et al."E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours".EUROPEAN JOURNAL OF CANCER 39.4(2003):517-523.
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