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学科主题: 临床医学
题名:
E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours
作者: Chen, CL; Liu, SS; Ip, SM; Wong, LC; Ng, TY; Ngan, HYS
关键词: E-cadherin ; methylation ; cervical cancer ; MSP ; methyltransferase ; antisense oligonucleotide
刊名: EUROPEAN JOURNAL OF CANCER
发表日期: 2003-03-01
卷: 39, 期:4, 页:517-523
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: METHYLTRANSFERASE EXPRESSION ; CPG METHYLATION ; COLON-CANCER ; CYTOSINE-DNA ; CARCINOMA ; GENE ; PROGRESSION ; HYPERMETHYLATION ; COMPLEX ; CATENINS
英文摘要:

A previous study showed E-cadherin expression was lost in some cervical cancer cell lines and tumours. This study was designed to clarify the significance of DNA methylation in silencing E-cadherin expression. We examined promoter methylation of E-cadherin in five cervical cancer cell lines and 20 cervical cancer tissues using methylation-specific PCR (MSP) and bisulphite DNA sequencing. The correlation of E-cadherin methylation and expression together with methyltransferase (DNMT1) were further studied. We found that hypermethylation of E-cadherin was involved in five cervical cancer cell lines and 40% (8/20) of cervical cancer tissues. E-cadherin protein was lost in 6/8 (75%) samples and 3/5 (60%) cell lines with promoter methylation. E-cadherin methylation was significantly correlated with increased DNMT1. Using an antisense DNMT1 oligo to transfect into SiHa HeLa C33A cell line, E-cadherin protein was re-expressed. We concluded that loss of E-cadherin expression was in part correlated with DNA methylation and DNMT1 expression in cervical cancer. (C) 2003 Elsevier Science Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000181686800026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62786
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Univ Hong Kong, Dept Obstet & Gynecol, Queen Mary Hosp, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R China

Recommended Citation:
Chen, CL,Liu, SS,Ip, SM,et al. E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours[J]. EUROPEAN JOURNAL OF CANCER,2003,39(4):517-523.
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