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Localization of Aristolochic Acid in Mouse Kidney Tissues by Immunohistochemistry Using an Anti-AA-I and AA-II Monoclonal Antibody
Li, Xiao-Wei1; Yokota, Sadaki4; Wang, Dan1,2; Wang, Xuan2; Shoyama, Yukihiro3; Cai, Shao-Qing1
关键词Aristolochic Acid Monoclonal Antibody Immunostaining Immunoperoxidase Light Microscopy Immunoelectron Microscopy Kidney
刊名AMERICAN JOURNAL OF CHINESE MEDICINE
2014
DOI10.1142/S0192415X14500918
42期:6页:1453-1469
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Integrative & Complementary Medicine ; Medicine, General & Internal
研究领域[WOS]Integrative & Complementary Medicine ; General & Internal Medicine
关键词[WOS]CHINESE HERBS NEPHROPATHY ; ORGANIC ANION TRANSPORTERS ; BALKAN ENDEMIC NEPHROPATHY ; TUBULAR EPITHELIAL-CELLS ; TOXICITY ; METABOLISM ; APOPTOSIS ; RATS ; NEPHROTOXICITY ; ACTIVATION
英文摘要

Aristolochic acids (AAs) are found in herbal medicines of Aristolochiaceae plants, including Aristolochia and Asarum species. AAs are associated with a rapidly progressive interstitial nephritis, which is called aristolochic acid nephropathy (AAN). However, the in-situ localization of AAs in the target organ, the kidney, has not been investigated yet. In the present study, the accumulation of aristolochic acid I (AA-I) in mouse kidney was revealed by immunoperoxidase light microscopy as well as colloidal gold immunoelectron microscopy (IEM) based on an anti-AA-I and AA-II monoclonal antibody (mAb). Male BALB/c mice were treated with 1.25 or 2.50 mg kg(-1) of AA-I per day for 5 days. Paraffin sections and ultra-thin sections of kidney tissue were respectively prepared. Under light microscopy, the apical surface of proximal tubules was strongly stained for AA-I, whereas no obvious immunostaining was found in the distal tubules and glomerulus, which remained relatively intact. Under electron microscopy, epithelial cells of the proximal tubules, distal tubules and collecting tubules were broken to various degrees. Gold labeling in the proximal and distal tubules was stronger than that in the collecting tubules. In renal tubules, immunogold signals of AA-I tended to accumulate in the mitochondria and peroxisomes, though the signals could be observed all over the cell. Gold signals were also found in the erythrocytes of glomeruli. The MAb against AA-I and AA-II provides a clue for the identification of proteins or factors which might interact with AA-I and thus induce targeted damage of kidney.

语种英语
WOS记录号WOS:000347026800011
项目编号2010601071 ; 30873466 ; 81173494 ; 2006BAI14B01
资助机构China Scholarship Council ; National Natural Science Foundation of China ; National Eleventh Five-year Key Technologies R&amp ; D Program of China ; Ministry of Education, Culture, Sports, Science and Technology, Japan
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62794
专题北京大学药学院_化学生物学系
作者单位1.Nagasaki Int Univ, Dept Pharmacognosy, Nagasaki 8593298, Japan
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
4.Nagasaki Int Univ, Fac Pharmaceut Sci, Sect Funct Morphol, Nagasaki 8593298, Japan
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Li, Xiao-Wei,Yokota, Sadaki,Wang, Dan,et al. Localization of Aristolochic Acid in Mouse Kidney Tissues by Immunohistochemistry Using an Anti-AA-I and AA-II Monoclonal Antibody[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2014,42(6):1453-1469.
APA Li, Xiao-Wei,Yokota, Sadaki,Wang, Dan,Wang, Xuan,Shoyama, Yukihiro,&Cai, Shao-Qing.(2014).Localization of Aristolochic Acid in Mouse Kidney Tissues by Immunohistochemistry Using an Anti-AA-I and AA-II Monoclonal Antibody.AMERICAN JOURNAL OF CHINESE MEDICINE,42(6),1453-1469.
MLA Li, Xiao-Wei,et al."Localization of Aristolochic Acid in Mouse Kidney Tissues by Immunohistochemistry Using an Anti-AA-I and AA-II Monoclonal Antibody".AMERICAN JOURNAL OF CHINESE MEDICINE 42.6(2014):1453-1469.
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