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学科主题: 药学
题名:
Localization of Aristolochic Acid in Mouse Kidney Tissues by Immunohistochemistry Using an Anti-AA-I and AA-II Monoclonal Antibody
作者: Li, Xiao-Wei1; Yokota, Sadaki4; Wang, Dan1,2; Wang, Xuan2; Shoyama, Yukihiro3; Cai, Shao-Qing1
关键词: Aristolochic Acid ; Monoclonal Antibody ; Immunostaining ; Immunoperoxidase Light Microscopy ; Immunoelectron Microscopy ; Kidney
刊名: AMERICAN JOURNAL OF CHINESE MEDICINE
发表日期: 2014
DOI: 10.1142/S0192415X14500918
卷: 42, 期:6, 页:1453-1469
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Integrative & Complementary Medicine ; Medicine, General & Internal
研究领域[WOS]: Integrative & Complementary Medicine ; General & Internal Medicine
关键词[WOS]: CHINESE HERBS NEPHROPATHY ; ORGANIC ANION TRANSPORTERS ; BALKAN ENDEMIC NEPHROPATHY ; TUBULAR EPITHELIAL-CELLS ; TOXICITY ; METABOLISM ; APOPTOSIS ; RATS ; NEPHROTOXICITY ; ACTIVATION
英文摘要:

Aristolochic acids (AAs) are found in herbal medicines of Aristolochiaceae plants, including Aristolochia and Asarum species. AAs are associated with a rapidly progressive interstitial nephritis, which is called aristolochic acid nephropathy (AAN). However, the in-situ localization of AAs in the target organ, the kidney, has not been investigated yet. In the present study, the accumulation of aristolochic acid I (AA-I) in mouse kidney was revealed by immunoperoxidase light microscopy as well as colloidal gold immunoelectron microscopy (IEM) based on an anti-AA-I and AA-II monoclonal antibody (mAb). Male BALB/c mice were treated with 1.25 or 2.50 mg kg(-1) of AA-I per day for 5 days. Paraffin sections and ultra-thin sections of kidney tissue were respectively prepared. Under light microscopy, the apical surface of proximal tubules was strongly stained for AA-I, whereas no obvious immunostaining was found in the distal tubules and glomerulus, which remained relatively intact. Under electron microscopy, epithelial cells of the proximal tubules, distal tubules and collecting tubules were broken to various degrees. Gold labeling in the proximal and distal tubules was stronger than that in the collecting tubules. In renal tubules, immunogold signals of AA-I tended to accumulate in the mitochondria and peroxisomes, though the signals could be observed all over the cell. Gold signals were also found in the erythrocytes of glomeruli. The MAb against AA-I and AA-II provides a clue for the identification of proteins or factors which might interact with AA-I and thus induce targeted damage of kidney.

语种: 英语
所属项目编号: 2010601071 ; 30873466 ; 81173494 ; 2006BAI14B01
项目资助者: China Scholarship Council ; National Natural Science Foundation of China ; National Eleventh Five-year Key Technologies R&amp ; D Program of China ; Ministry of Education, Culture, Sports, Science and Technology, Japan
WOS记录号: WOS:000347026800011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62794
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Nagasaki Int Univ, Dept Pharmacognosy, Nagasaki 8593298, Japan
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
4.Nagasaki Int Univ, Fac Pharmaceut Sci, Sect Funct Morphol, Nagasaki 8593298, Japan

Recommended Citation:
Li, Xiao-Wei,Yokota, Sadaki,Wang, Dan,et al. Localization of Aristolochic Acid in Mouse Kidney Tissues by Immunohistochemistry Using an Anti-AA-I and AA-II Monoclonal Antibody[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2014,42(6):1453-1469.
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