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学科主题: 药学
题名:
Surface Properties of Lipoplexes Modified with Mannosylerythritol Lipid-A and Tween 80 and Their Cellular Association
作者: Ding, Wuxiao1; Hattori, Yoshiyuki1; Qi, Xianrong2; Kitamoto, Dai3; Maitani, Yoshie1
关键词: cationic liposome ; lipoplex ; surfactant ; surface pH ; hydration
刊名: CHEMICAL & PHARMACEUTICAL BULLETIN
发表日期: 2009-02-01
卷: 57, 期:2, 页:138-143
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: CATIONIC CHOLESTEROL DERIVATIVES ; GENE DELIVERY ; LAURDAN FLUORESCENCE ; LIPOSOMES ; BIOSURFACTANT ; TRANSFECTION ; HYDRATION ; DNA ; COMPLEXES ; PATHWAY
英文摘要:

The surface properties of cationic liposomes and lipoplexes largely determine the cellular association and gene transfection efficiency. In this study, we measured the surface properties, such as zeta potentials, surface pH and hydration levels of MHAPC- and OH-Chol-lipoplexes and their cellular association, without and with the modification of biosurfactant mannosylerythritol lipid-A (MEL-A) or Tween 80 (MHAPC=N,N-methyl hydroxyethyl aminopropane carbamoyl cholesterol; OH-Chol=cholesteryl-3 beta-carboxyamindoethylene-N-hydroxyethylamine). Compared to OH-Chol-lipoplexes, the higher cellular association of MHAPC-lipoplexes correlated with the significantly higher zeta potentials, lower surface pH levels and "drier" surface, as evaluated by the generalized polarization of laurdan. Both MEL-A and Tween 80 modification of MHAPC-lipoplexes did not significantly change zeta potentials and surface pH levels, while MEL-A modification of OH-Chol-lipoplexes seriously decreased them. MEL-A hydrated the liposomal surface of MHAPC-lipoplexes but dehydrated that of OH-Chol-lipoplexes, while Tween 80 hydrated those of MHAPC- and OH-Chol-lipoplexes. In all, cationic liposomes composed of lipids with secondary and tertiary amine exhibited different surface properties and cellular associations of lipoplexes, and modification with surfactants further enlarged their difference. The strong hydration ability of Tween 80 may relate to the low cellular association of lipoplexes, while the dehydration of MEL-A-modified OH-Chol-lipoplexes seemed to compensate the negative zeta potential for the cellular association of lipoplexes.

语种: 英语
项目资助者: Ministry of Health, Labor and welfare ; Open Research Center Project
WOS记录号: WOS:000262842000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62798
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Hoshi Univ, Inst Med Chem, Shinagawa Ku, Tokyo 1428501, Japan
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
3.Natl Inst Adv Ind Sci & Technol, AIST Tsukuba Cent, Res Inst Innovat Sustainable Chem, Tsukuba, Ibaraki 3058565, Japan

Recommended Citation:
Ding, Wuxiao,Hattori, Yoshiyuki,Qi, Xianrong,et al. Surface Properties of Lipoplexes Modified with Mannosylerythritol Lipid-A and Tween 80 and Their Cellular Association[J]. CHEMICAL & PHARMACEUTICAL BULLETIN,2009,57(2):138-143.
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