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Changes in conformation of human neuronal tau during denaturation in formaldehyde solution
Nie, CL; Zhang, W; Zhang, D; He, RQ
关键词human neuronal tau 8-anilino-l-naphthalenesulfonic acid (ANS) tau aggregation denaturation formaldehyde
刊名PROTEIN AND PEPTIDE LETTERS
2005
12期:1页:75-+
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PAIRED HELICAL FILAMENT ; PROTEIN-TAU ; ALZHEIMERS-DISEASE ; PHOSPHORYLATION ; AGGREGATION ; ISOFORMS ; BETA ; MODEL ; DNA
英文摘要

Human neuronal tau was incubated in formaldehyde solution at low concentrations and the intensity of light scattering of tau-40 solution at 480 nm increased markedly. Then potassium iodide was used to quench the intrinsic fluorescence of tau. The fluorescent quenching constants decreased as formaldehyde concentrations increased. 8-anilino1-naphthalenesulfonic acid (ANS) binding assay showed that a putative hydrophobic core formed in tau polymers during incubation with formaldehyde. Native tau was hydrolyzed by immobilized earthworm fibrinolytic enzyme-II (EFE-II), producing a digested fragment (36-37 kDa). However, formaldehyde-treated tau could not be digested under the same conditions, suggesting that aggregated protein was relatively rigidly deposited.

语种英语
WOS记录号WOS:000226057400011
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62800
专题北京大学精神卫生研究所
作者单位1.Beijing Univ, Chinese Acad Sci, Inst Biophys, Lab Visual Infromat Proc, Beijing 100871, Peoples R China
2.Beijing Univ, Inst Mental Hlth, Beijing 100871, Peoples R China
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GB/T 7714
Nie, CL,Zhang, W,Zhang, D,et al. Changes in conformation of human neuronal tau during denaturation in formaldehyde solution[J]. PROTEIN AND PEPTIDE LETTERS,2005,12(1):75-+.
APA Nie, CL,Zhang, W,Zhang, D,&He, RQ.(2005).Changes in conformation of human neuronal tau during denaturation in formaldehyde solution.PROTEIN AND PEPTIDE LETTERS,12(1),75-+.
MLA Nie, CL,et al."Changes in conformation of human neuronal tau during denaturation in formaldehyde solution".PROTEIN AND PEPTIDE LETTERS 12.1(2005):75-+.
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