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学科主题: 药学
题名:
Metal-Free DNA Linearized Nuclease Based on PASP-Polyamine Conjugates
作者: Li, Chao1; Zhao, Fangfang1; Huang, Yunan1; Liu, Xueyuan1; Liu, Yan3; Qiao, Renzhong1,2; Zhao, Yufen3
刊名: BIOCONJUGATE CHEMISTRY
发表日期: 2012-09-01
DOI: 10.1021/bc300162g
卷: 23, 期:9, 页:1832-1837
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: DOUBLE-STRAND BREAKS ; L-ASPARTIC ACID ; CLEAVAGE ACTIVITY ; SERYL-HISTIDINE ; POLY(SUCCINIMIDE) ; POLYCONDENSATION ; METALLONUCLEASES ; GUANIDINIUM ; DIPEPTIDE
英文摘要:

Genome manipulation controlled by small metal complexes has attracted extensive interest because of their potential application in the fields of molecular biotechnology and drug development. However, their medicinal application is still limited due to the distinct toxicity of the free radicals generated by partial metal complexes based on oxidative cleaving processes. Thus, it is still a challenge for US to use metal free agent to cleave DNA. In this work, we showed that a family of polyamine-grafted PASP (poly(aspartic acid)) conjugates is able to rapidly induce DNA cleavage in the absence of metal ions, and obtain a high-yield linearization product via a hydrolytic path. From the results of detailed control experiments, it was revealed that the formation of polyamine cation/phosphate anion pair and free ungrafted nucleophilic groups would be the key factors to improve DNA linearization. Constructing polyamine conjugates based on short peptide such as polyamine-grafted PASP, as achieved here, could provide an attractive strategy for developing mild and efficient artificial nucleases as well as researching catalytic mechanisms on DNA chemistry.

语种: 英语
所属项目编号: 20972014 ; 21202005 ; 21232005 ; 21172016.
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000308833600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62817
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China

Recommended Citation:
Li, Chao,Zhao, Fangfang,Huang, Yunan,et al. Metal-Free DNA Linearized Nuclease Based on PASP-Polyamine Conjugates[J]. BIOCONJUGATE CHEMISTRY,2012,23(9):1832-1837.
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