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Metal-Free DNA Linearized Nuclease Based on PASP-Polyamine Conjugates
Li, Chao1; Zhao, Fangfang1; Huang, Yunan1; Liu, Xueyuan1; Liu, Yan3; Qiao, Renzhong1,2; Zhao, Yufen3
刊名BIOCONJUGATE CHEMISTRY
2012-09-01
DOI10.1021/bc300162g
23期:9页:1832-1837
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DOUBLE-STRAND BREAKS ; L-ASPARTIC ACID ; CLEAVAGE ACTIVITY ; SERYL-HISTIDINE ; POLY(SUCCINIMIDE) ; POLYCONDENSATION ; METALLONUCLEASES ; GUANIDINIUM ; DIPEPTIDE
英文摘要

Genome manipulation controlled by small metal complexes has attracted extensive interest because of their potential application in the fields of molecular biotechnology and drug development. However, their medicinal application is still limited due to the distinct toxicity of the free radicals generated by partial metal complexes based on oxidative cleaving processes. Thus, it is still a challenge for US to use metal free agent to cleave DNA. In this work, we showed that a family of polyamine-grafted PASP (poly(aspartic acid)) conjugates is able to rapidly induce DNA cleavage in the absence of metal ions, and obtain a high-yield linearization product via a hydrolytic path. From the results of detailed control experiments, it was revealed that the formation of polyamine cation/phosphate anion pair and free ungrafted nucleophilic groups would be the key factors to improve DNA linearization. Constructing polyamine conjugates based on short peptide such as polyamine-grafted PASP, as achieved here, could provide an attractive strategy for developing mild and efficient artificial nucleases as well as researching catalytic mechanisms on DNA chemistry.

语种英语
WOS记录号WOS:000308833600014
项目编号20972014 ; 21202005 ; 21232005 ; 21172016.
资助机构National Nature Science Foundation of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62817
专题北京大学药学院
北京大学第一临床医学院_介入血管外科
作者单位1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China
推荐引用方式
GB/T 7714
Li, Chao,Zhao, Fangfang,Huang, Yunan,et al. Metal-Free DNA Linearized Nuclease Based on PASP-Polyamine Conjugates[J]. BIOCONJUGATE CHEMISTRY,2012,23(9):1832-1837.
APA Li, Chao.,Zhao, Fangfang.,Huang, Yunan.,Liu, Xueyuan.,Liu, Yan.,...&Zhao, Yufen.(2012).Metal-Free DNA Linearized Nuclease Based on PASP-Polyamine Conjugates.BIOCONJUGATE CHEMISTRY,23(9),1832-1837.
MLA Li, Chao,et al."Metal-Free DNA Linearized Nuclease Based on PASP-Polyamine Conjugates".BIOCONJUGATE CHEMISTRY 23.9(2012):1832-1837.
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