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学科主题: 基础医学
题名:
Effect of murine thymic epithelial cell line (MTEC1) on the functional expression of CD4(+)CD8(-) thymocyte subgroups
作者: Ge, Q; Chen, WF
关键词: CD4(+)CD8(-) thymocyte subgroup ; cytokine ; proliferation ; thymic stromal cells
刊名: INTERNATIONAL IMMUNOLOGY
发表日期: 2000-08-01
卷: 12, 期:8, 页:1127-1133
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: T-CELLS ; LYMPHOKINE SECRETION ; NEGATIVE SELECTION ; MATURATION ; HELPER ; SUBSETS ; CD4+ ; MICE ; ACQUISITION ; PATTERNS
英文摘要:

To determine the effect of thymic stromal cells on the functional maturation of CD4 single-positive (SP) thymocytes, the functional status of isolated CD4 SP thymocyte subgroups was investigated by means of cell proliferation and cytokine production in response to concanavalin A (Con A) prior and after co-culturing with a murine thymic epithelial cell line (MTEC1). Mouse medullary CD4 SP thymocytes were phenotypically divided into seven discrete subgroups predicted to reflect the maturation pathway from newly emerging CD4 SP thymocytes to terminally differentiated cells. For functional analysis, six major subgroups (6C10(+)CD69(+), 6C10(-)CD69(+), 6C10(-)CD69(-)3G11(+)Qa-2(-), 6C10(-)CD69(-)3G11(+)Qa-2(+), 6C10(-)CD69(-)3G11(-)Qa-2(-) and 6C10(-)CD69(-)3G11(-)Qa-(2+)) cells were isolated and their functional status in response to Con A stimulation assessed. A functional hierarchy is revealed among these subgroups, consistent with their phenotypic maturation status, which may imply that these cells undergo a functional maturation process within thymic medulla, The function of cytokine production by CD4 SP thymocytes is acquired in a stepwise manner from a low to high level and characterized by T(h)0-type cytokines in the main stream of differentiation pathway. However, a minor subgroup that appeared at the late stage as 3G11-6C10(-) cells was biased to produce T(h)2-type cytokines. Nevertheless, the functional capacity of the final two Qa-2(+) subgroups of CD4 SP thymocytes was still significantly lower than that of spleen CD4(+) T cells. After co-cultivation with MTEC1 cells, four subgroups of TCR alpha beta(+)CD4(+)CD8(-) thymocytes exhibited significantly higher levels of proliferation capability and modulation in cytokine production capability. However, co-culturing with MTEC1 cells did not change the pattern of T(h)0- or T(h)2-like cytokine production by respectively medullary CD4 SP thymocyte subgroups nor could MTEC1 induce CD4 SP thymocytes to secrete T(h)1-type cytokines, The results suggest that MTEC1 can regulate the functional status of these thymocyte subgroups.

语种: 英语
WOS记录号: WOS:000088741900002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62823
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Ge, Q,Chen, WF. Effect of murine thymic epithelial cell line (MTEC1) on the functional expression of CD4(+)CD8(-) thymocyte subgroups[J]. INTERNATIONAL IMMUNOLOGY,2000,12(8):1127-1133.
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