IR@PKUHSC  > 北京大学药学院
学科主题药学
Anti-Neuroinflammatory Effect of MC13, a Novel Coumarin Compound From Condiment Murraya, Through Inhibiting Lipopolysaccharide-Induced TRAF6-TAK1-NF-B, P38/ERK MAPKS and Jak2-Stat1/Stat3 Pathways
Zeng, Ke-Wu1; Yu, Qian2; Liao, Li-Xi1; Song, Fang-Jiao2; Lv, Hai-Ning1; Jiang, Yong1; Tu, Peng-Fei1
关键词NEUROINFLAMMATION MICROGLIA COUMARIN NF-B MAPK JAK STAT
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2015-07-01
DOI10.1002/jcb.25084
116期:7页:1286-1299
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Key Technology R&amp ; D Program "New Drug Innovation" of China ; Natural Science Foundation of China ; National Key Technology R&amp ; D Program "New Drug Innovation" of China ; Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]NF-KAPPA-B ; ADJUVANT-INDUCED ARTHRITIS ; ISCHEMIC BRAIN-INJURY ; BV2 MICROGLIAL CELLS ; TOLL-LIKE RECEPTORS ; NATURAL COUMARIN ; OXIDATIVE DAMAGE ; ACTIVATION ; INFLAMMATION ; P38
英文摘要

MC13 is a novel coumarin compound found in Murraya, an economic crop whose leaves are widely used as condiment (curry) in cuisine. The aims of the present study were to investigate the neuroprotective effects of MC13 on microglia-mediated inflammatory injury model as well as potential molecular mechanism. Cell viability and apoptosis assay demonstrated that MC13 was not toxic to neurons and significantly protected neurons from microglia-mediated inflammatory injury upon lipopolysaccharide (LPS) stimulation. Results showed that MC13 markedly inhibited LPS-induced production of various inflammatory mediators, including nitrite oxide (Griess method), TNF- and IL-6 (ELISA assay) in a concentration-dependent manner. Mechanism study showed that MC13 could suppress the activation of NF-B, which was the central regulator for inflammatory response, and also decreased the interaction of TGF--activated kinase 1 (TAK1)-binding protein (TAB2) with TAK1 and TNF receptor associated factor (TRAF6), leading to the decreased phosphorylation levels of NF-B upstream regulators such as IB and IB kinase (IKK). MC13 also significantly down-regulated the phosphorylation levels of ERK and p38 MAPKs, which played key roles in microglia-mediated inflammatory response. Furthermore, MC13 inhibited Jak2-dependent Stat1/3 signaling pathway activation by blocking Jak2 phosphorylation, Stat1/3 phosphorylation, and nuclear translocation. Taken together, our results demonstrated that MC13 protected neurons from microglia-mediated neuroinflammatory injury by inhibiting TRAF6-TAK1-NF-B, p38/ERK MAPKs, and Jak2-Stat1/3 pathways. Finally, MC13 might interact with LPS and interfere LPS-binding to cell membrane surface. These findings suggested that coumarin might act as a potential medicinal agent for treating neuroinflammation as well as inflammation-related neurodegenerative diseases. J. Cell. Biochem. 116: 1286-1299, 2015. (c) 2015 Wiley Periodicals, Inc.

语种英语
所属项目编号2012ZX09301002-002-002 ; 2012ZX09304-005 ; 81303253 ; 30873072 ; 81222051 ; 81473106
资助者National Key Technology R&amp ; D Program "New Drug Innovation" of China ; Natural Science Foundation of China ; National Key Technology R&amp ; D Program "New Drug Innovation" of China ; Natural Science Foundation of China
WOS记录号WOS:000354457900014
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62826
Collection北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 1, Res Studio Integrat Tradit & Western Med, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Ke-Wu,Yu, Qian,Liao, Li-Xi,et al. Anti-Neuroinflammatory Effect of MC13, a Novel Coumarin Compound From Condiment Murraya, Through Inhibiting Lipopolysaccharide-Induced TRAF6-TAK1-NF-B, P38/ERK MAPKS and Jak2-Stat1/Stat3 Pathways[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2015,116(7):1286-1299.
APA Zeng, Ke-Wu.,Yu, Qian.,Liao, Li-Xi.,Song, Fang-Jiao.,Lv, Hai-Ning.,...&Tu, Peng-Fei.(2015).Anti-Neuroinflammatory Effect of MC13, a Novel Coumarin Compound From Condiment Murraya, Through Inhibiting Lipopolysaccharide-Induced TRAF6-TAK1-NF-B, P38/ERK MAPKS and Jak2-Stat1/Stat3 Pathways.JOURNAL OF CELLULAR BIOCHEMISTRY,116(7),1286-1299.
MLA Zeng, Ke-Wu,et al."Anti-Neuroinflammatory Effect of MC13, a Novel Coumarin Compound From Condiment Murraya, Through Inhibiting Lipopolysaccharide-Induced TRAF6-TAK1-NF-B, P38/ERK MAPKS and Jak2-Stat1/Stat3 Pathways".JOURNAL OF CELLULAR BIOCHEMISTRY 116.7(2015):1286-1299.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Zeng, Ke-Wu]'s Articles
[Yu, Qian]'s Articles
[Liao, Li-Xi]'s Articles
百度学术
百度学术Similar articles in
[Zeng, Ke-Wu]'s Articles
[Yu, Qian]'s Articles
[Liao, Li-Xi]'s Articles
必应学术
必应学术Similar articles in
[Zeng, Ke-Wu]'s Articles
[Yu, Qian]'s Articles
[Liao, Li-Xi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.