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学科主题: 基础医学
题名:
Overexpression of heat-shock protein 20 in rat heart myogenic cells confers protection against simulated ischemia/reperfusion injury
作者: Zhu, YH; Wang, X
关键词: reperfusion injury ; adenovirus ; apoptosis ; necrosis ; heat-shock proteins
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2005-09-01
DOI: 10.1111/j.1745-7254.2005.00137.x
卷: 26, 期:9, 页:1076-1080
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: ALPHA-B-CRYSTALLIN ; PROTEIN-KINASE-C ; SHOCK-PROTEIN ; HSP27 GENE ; EXPRESSION ; STRESS ; THERMORESISTANCE ; RESISTANCE ; MUSCLE
英文摘要:

Aim: To explore whether overexpression of the small heat shock protein HSP20 in rat cardiomyocytes protects against simulated ischemia/reperfusion (SI/R) injury. Methods: Recombinant adenovirus expressing HSP20 was used to infect rat H9c2 cardiomyocytes at high efficiency, as assessed by green fluorescent protein. H9c2 cells were subjected to SI/R stress; survival was estimated through assessment of lactate dehydrogenase and cell apoptosis through caspase-3 activity. Results: Overexpression of HSP20 decreased lactate dehydrogenase release by 21.5% and caspase-3 activity by 58.8%. Pretreatment with the protein kinase C inhibitor Ro-31-8220 (0.1 mu mol/L) for 30 min before SI/R canceled the protective effect of HSP20. The selective mitochondrial K-ATP(+), channel inhibitor 5-hydroxydecanoate (100 mu mol/L) had a similar effect. However, the non-selective K-ATP(+), channel inhibitor glibenclamide (100 mu mol/L) had no significant effect. Conclusion: These data indicate that the protective effect of HSP20 in vitro is primarily due to reduced necrotic and apoptotic death of cardiomyocytes, possibly via the protein kinase C/mitochondrial K(+)ATP pathway.

语种: 英语
WOS记录号: WOS:000231833000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62835
Appears in Collections:基础医学院_期刊论文

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作者单位: Peking Univ, Minist Educ, Basic Med Coll, Dept Physiol & Pathophysiol,Key Lab Mol Cardiovas, Beijing 100083, Peoples R China

Recommended Citation:
Zhu, YH,Wang, X. Overexpression of heat-shock protein 20 in rat heart myogenic cells confers protection against simulated ischemia/reperfusion injury[J]. ACTA PHARMACOLOGICA SINICA,2005,26(9):1076-1080.
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