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Interactions between thrombin with flavonoids from Abelmoschus manihot (L.) Medicus by CZE
Liu, Yi; Lai, Xianyin; Ling, Xiaomei; Zhao, Yuying; Cui, Jingrong
关键词capillary zone electrophoresis Abelmoschus manihot (L.) Medicus flavonoid thrombin
刊名CHROMATOGRAPHIA
2006-07-01
DOI10.1365/s10337-006-0841-7
64期:1-2页:45-50
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]AFFINITY CAPILLARY-ELECTROPHORESIS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; INDUCED FLUORESCENCE DETECTION ; ZONE-ELECTROPHORESIS ; FRONTAL ANALYSIS ; SERUM-ALBUMIN ; ENANTIOSELECTIVE BINDING ; MOLECULAR-INTERACTIONS ; PLASMA-LIPOPROTEINS ; PROTEIN
英文摘要

A capillary zone electrophoretic method was applied to determine the interactions between flavonoids from Abelmoschus manihot (L.) Medicus and thrombin. Samples containing flavonoids and thrombin at various ratios were incubated at 25 degrees C and then were separated by CZE with tris-acetate buffer at pH 7.2. Each run could be accomplished within 10 min. In CZE, the peak width broadened due to the affinity interactions between flavonoids and thrombin. Compared with positive and negative control, hirudin interacted with thrombin but heparin had no binding to thrombin, we concluded that the total flavone LXY1 and flavonoids LXY3, LXY4, LXY5 and LXY7 from Abelmoschus manihot (L.) Medicus interacted with thrombin; the aqueous extract LXY2 and flavonoid LXY6 had no binding to thrombin. Both qualification and quantification characterizations of the binding were determined. The experimental results showed that the reported method by capillary zone electrophoresis for the determination of flavonoids and thrombin interactions is powerful, sensitive and fast, requires less amounts of reagents, and further, it can be employed as a reliable alternative to other methods.

语种英语
WOS记录号WOS:000239677700007
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62841
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yi,Lai, Xianyin,Ling, Xiaomei,et al. Interactions between thrombin with flavonoids from Abelmoschus manihot (L.) Medicus by CZE[J]. CHROMATOGRAPHIA,2006,64(1-2):45-50.
APA Liu, Yi,Lai, Xianyin,Ling, Xiaomei,Zhao, Yuying,&Cui, Jingrong.(2006).Interactions between thrombin with flavonoids from Abelmoschus manihot (L.) Medicus by CZE.CHROMATOGRAPHIA,64(1-2),45-50.
MLA Liu, Yi,et al."Interactions between thrombin with flavonoids from Abelmoschus manihot (L.) Medicus by CZE".CHROMATOGRAPHIA 64.1-2(2006):45-50.
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