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学科主题: 临床医学
题名:
Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines
作者: Ji, JF; Chen, X; Leung, SY; Chi, JTA; Chu, KM; Yuen, ST; Li, R; Chan, ASY; Li, JY; Dunphy, N; So, S
关键词: gene expression profile ; microarray ; gastric cancer
刊名: ONCOGENE
发表日期: 2002-09-19
DOI: 10.1038/sj.onc.1205829
卷: 21, 期:42, 页:6549-6556
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity
关键词[WOS]: PREDICTION ; METAPLASIA ; PATTERNS ; DATABASE
英文摘要:

Gastric adenocarcinoma is one of the major malignancies worldwide. Gastric cell lines have been widely used as the model to study the genetics, pharmacology and biochemistry of gastric cancers. Here we describe a comprehensive survey of the gene expression profiles of 12 gastric carcinoma cell lines, using cDNA microarray with 43000 clones. For comparison, we also explored the gene expression patterns of 15 cell lines derived from lymphoid, endothelial, stromal and other epithelial cancers. Expression levels of specific genes were validated through comparison to protein expression by immunohistochemistry using cell block arrays. We found sets of genes whose expression corresponds to the molecular signature of each cell type. In the gastric cancer cell lines, apart from genes that are highly expressed corresponding to their common epithelial origin from the gastrointestinal tract, we found marked heterogeneity among the gene expression patterns of these cell lines. Some of the heterogeneity may reflect their underlying molecular characteristics or specific differentiation program. Two putative gastric carcinoma cell lines were found to be B-cell lymphoma, and another one had no epithelial specific gene expression and hence was of doubtful epithelial origin. These cell lines should no longer be used in gastric carcinoma research. In conclusion, our gene expression database can serve as a powerful resource for the study of gastric cancer using these cell lines.

语种: 英语
WOS记录号: WOS:000177925300015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62851
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Stanford Univ, Dept Biochem, Stanford, CA 94305 USA
2.Stanford Univ, Dept Genet, Stanford, CA 94305 USA
3.Stanford Univ, Med Ctr, Dept Surg, Stanford, CA 94305 USA
4.Stanford Univ, Asian Liver Ctr, Stanford, CA 94305 USA
5.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Surg, Beijing 100871, Peoples R China
6.Univ Hong Kong, Queen Mary Hosp, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
7.Univ Hong Kong, Queen Mary Hosp, Dept Surg, Hong Kong, Hong Kong, Peoples R China
8.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Ji, JF,Chen, X,Leung, SY,et al. Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines[J]. ONCOGENE,2002,21(42):6549-6556.
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