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Pyrocatechol violet as a marker to characterize liposomal membrane permeability using the chelation and the first-order derivative spectrophotometry
Jin, YG; Li, M; Hou, XP
关键词pyrocatechol violet chelation derivative spectrophotometry liposomes membrane permeability
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2005-02-23
DOI10.1016/j.jpba.2004.10.019
37期:2页:379-382
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
英文摘要

Pyrocatechol violet (PV), a chelating agent for cupric ions was used to characterize liposomal membrane permeability. After cupric ions were added to PV liposomes. free PV turned into its chelate (PV-Cu), and encapsulated PV kept stable since liposomal membranes prevented metal ions from permeating. After the light scattering background of liposomes and the absorbance of PV were eliminated by the first-order derivative spectrophotometric method, PV-Cu i.e. free PV in liposome Suspensions could be determined without separation. The released PV from liposomes could also be determined. Because PV release is relevant to liposomal membrane permeability, PV becomes a marker to characterize the membrane permeability. This new method was simple, rapid, sensitive, and was used to measure the temperature-dependent liposomal membrane permeability in this paper. Dipalmitoylphosphatidylcholine (DPPC) and soybean lecithin liposomes showed the peaks of release at 40 degreesC and 39 degreesC, respectively. (C) 2004 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000227482100023
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62855
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Phys Pharm, Beijing 100083, Peoples R China
2.Beijing Inst Radiat Med, Dept Pharmaceut Chem, Beijing 100850, Peoples R China
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Jin, YG,Li, M,Hou, XP. Pyrocatechol violet as a marker to characterize liposomal membrane permeability using the chelation and the first-order derivative spectrophotometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,37(2):379-382.
APA Jin, YG,Li, M,&Hou, XP.(2005).Pyrocatechol violet as a marker to characterize liposomal membrane permeability using the chelation and the first-order derivative spectrophotometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,37(2),379-382.
MLA Jin, YG,et al."Pyrocatechol violet as a marker to characterize liposomal membrane permeability using the chelation and the first-order derivative spectrophotometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 37.2(2005):379-382.
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