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学科主题: 药学
题名:
Pyrocatechol violet as a marker to characterize liposomal membrane permeability using the chelation and the first-order derivative spectrophotometry
作者: Jin, YG; Li, M; Hou, XP
关键词: pyrocatechol violet ; chelation ; derivative spectrophotometry ; liposomes ; membrane permeability
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2005-02-23
DOI: 10.1016/j.jpba.2004.10.019
卷: 37, 期:2, 页:379-382
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
英文摘要:

Pyrocatechol violet (PV), a chelating agent for cupric ions was used to characterize liposomal membrane permeability. After cupric ions were added to PV liposomes. free PV turned into its chelate (PV-Cu), and encapsulated PV kept stable since liposomal membranes prevented metal ions from permeating. After the light scattering background of liposomes and the absorbance of PV were eliminated by the first-order derivative spectrophotometric method, PV-Cu i.e. free PV in liposome Suspensions could be determined without separation. The released PV from liposomes could also be determined. Because PV release is relevant to liposomal membrane permeability, PV becomes a marker to characterize the membrane permeability. This new method was simple, rapid, sensitive, and was used to measure the temperature-dependent liposomal membrane permeability in this paper. Dipalmitoylphosphatidylcholine (DPPC) and soybean lecithin liposomes showed the peaks of release at 40 degreesC and 39 degreesC, respectively. (C) 2004 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000227482100023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62855
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Phys Pharm, Beijing 100083, Peoples R China
2.Beijing Inst Radiat Med, Dept Pharmaceut Chem, Beijing 100850, Peoples R China

Recommended Citation:
Jin, YG,Li, M,Hou, XP. Pyrocatechol violet as a marker to characterize liposomal membrane permeability using the chelation and the first-order derivative spectrophotometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,37(2):379-382.
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