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学科主题: 基础医学
题名:
Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins
作者: Liang, Chao1,2; Zhang, Xueli2; Song, Shanshan2,3; Tian, Chunyan2; Yin, Yuxin4; Xing, Guichun2; He, Fuchu2; Zhang, Lingqiang1,2
关键词: MPG ; UHRF1 ; UHRF2 ; IP/MS ; Protein interaction
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2013-04-19
DOI: 10.1016/j.bbrc.2013.02.126
卷: 433, 期:4, 页:415-419
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: BASE EXCISION-REPAIR ; CELL-CYCLE ARREST ; ALKYLATING-AGENTS ; CANCER ; METHYLATION ; SENSITIVITY ; ICBP90 ; TARGET ; DNMT1 ; LINKS
英文摘要:

N-methylpurine DNA glycosylase (MPG), a DNA repair enzyme, functions in the DNA base excision repair (BER) pathway. Aberrant over-expression of MPG in various cancers suggests an important role of MPG in carcinogenesis. Identification of MPG-interacting proteins will help to dissect the molecular link between MPG and cancer development. In the present study, using immunoprecipitation coupled with mass spectrometry (IP/MS), we screened ubiquitin-like, containing PHD and RING finger domains 1 (UHRF1), an essential protein required for the maintenance of DNA methylation, as a MPG-interacting protein. Endogenous co-immunoprecipitation assay in cancer cells confirmed that UHRF1 interacted with MPG in a p53 status-independent manner. Confocal microscopy showed that endogenous MPG and UHRF1 were co-localized in the nucleoplasm. Furthermore, co-immunoprecipitation assay indicated that UHRF2, the homolog of UHRF1, could also interact with MPG. These results show that MPG and the UHRF family of proteins interact, thus providing a functional linkage between MPG and UHRF1/2. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 2011CB910802 ; 2010CB912202 ; 31071144 ; 31125010 ; 81221004
项目资助者: National Basic Research Programs ; National Natural Science Foundation Projects
WOS记录号: WOS:000318259100011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62868
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Anhui Med Univ, Dept Cell Biol, Hefei 230032, Anhui, Peoples R China
2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 100850, Peoples R China
3.Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA
4.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Liang, Chao,Zhang, Xueli,Song, Shanshan,et al. Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,433(4):415-419.
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