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学科主题基础医学
Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins
Liang, Chao1,2; Zhang, Xueli2; Song, Shanshan2,3; Tian, Chunyan2; Yin, Yuxin4; Xing, Guichun2; He, Fuchu2; Zhang, Lingqiang1,2
关键词MPG UHRF1 UHRF2 IP/MS Protein interaction
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2013-04-19
DOI10.1016/j.bbrc.2013.02.126
433期:4页:415-419
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]BASE EXCISION-REPAIR ; CELL-CYCLE ARREST ; ALKYLATING-AGENTS ; CANCER ; METHYLATION ; SENSITIVITY ; ICBP90 ; TARGET ; DNMT1 ; LINKS
英文摘要

N-methylpurine DNA glycosylase (MPG), a DNA repair enzyme, functions in the DNA base excision repair (BER) pathway. Aberrant over-expression of MPG in various cancers suggests an important role of MPG in carcinogenesis. Identification of MPG-interacting proteins will help to dissect the molecular link between MPG and cancer development. In the present study, using immunoprecipitation coupled with mass spectrometry (IP/MS), we screened ubiquitin-like, containing PHD and RING finger domains 1 (UHRF1), an essential protein required for the maintenance of DNA methylation, as a MPG-interacting protein. Endogenous co-immunoprecipitation assay in cancer cells confirmed that UHRF1 interacted with MPG in a p53 status-independent manner. Confocal microscopy showed that endogenous MPG and UHRF1 were co-localized in the nucleoplasm. Furthermore, co-immunoprecipitation assay indicated that UHRF2, the homolog of UHRF1, could also interact with MPG. These results show that MPG and the UHRF family of proteins interact, thus providing a functional linkage between MPG and UHRF1/2. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000318259100011
项目编号2011CB910802 ; 2010CB912202 ; 31071144 ; 31125010 ; 81221004
资助机构National Basic Research Programs ; National Natural Science Foundation Projects
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62868
专题北京大学基础医学院_病理学系
北京大学基础医学院
作者单位1.Anhui Med Univ, Dept Cell Biol, Hefei 230032, Anhui, Peoples R China
2.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 100850, Peoples R China
3.Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA
4.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Liang, Chao,Zhang, Xueli,Song, Shanshan,et al. Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,433(4):415-419.
APA Liang, Chao.,Zhang, Xueli.,Song, Shanshan.,Tian, Chunyan.,Yin, Yuxin.,...&Zhang, Lingqiang.(2013).Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,433(4),415-419.
MLA Liang, Chao,et al."Identification of UHRF1/2 as new N-methylpurine DNA glycosylase-interacting proteins".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 433.4(2013):415-419.
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