学科主题临床医学
A circulating miRNA signature as a diagnostic biomarker for non-invasive early detection of breast cancer
Zhang, Lei1; Xu, Ye2; Jin, Xingyu3; Wang, Zengwu4; Wu, Yidi1; Zhao, Deyao1; Chen, Gang5; Li, Deyu5; Wang, Xiaoxia1; Cao, Huiqing1; Xie, Yuntao2; Liang, Zicai1
关键词Circulating miRNA Serum direct detection Breast cancer Diagnosis
刊名BREAST CANCER RESEARCH AND TREATMENT
2015-11-01
DOI10.1007/s10549-015-3591-0
154期:2页:423-434
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National High-Tech R&amp ; D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Jiangsu ; 985 Project of Peking University ; National High-Tech R&amp ; D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Jiangsu ; 985 Project of Peking University
研究领域[WOS]Oncology
关键词[WOS]BLOOD-BASED MARKERS ; SCREENING MAMMOGRAPHY ; MICRORNA EXPRESSION ; HEPATOCELLULAR-CARCINOMA ; SERUM ; TUMOR ; CELLS ; IDENTIFICATION ; ACCURACY ; TARGETS
英文摘要

Novel, non-invasive biomarkers to diagnose breast cancer with high sensitivity and specificity are greatly desired. Circulating microRNAs (miRNAs) show potential for breast cancer detection, but the existing results appear to be mixed. Using microscale serum, we established a novel serum-direct multiplex detection assay based on RT-PCR (SdM-RT-PCR). Ninety-three miRNAs dysregulated or with functions in breast cancer were selected as candidates, and additional 3 miRNAs were chosen as endogenous controls. We first conducted miRNA profiling of these 96 miRNAs by SdM-RT-PCR using the sera of 25 breast cancer patients at diagnosis prior to treatment and 20 age-matched healthy controls. miRNAs showing significantly different expression levels between patients and controls were further analyzed using a logistic regression model. A miRNA signature was validated in an independent set of 128 serum samples composed of 76 breast cancer patients and 52 healthy controls. In the discovery stage, we identified 23 miRNAs as significantly dysregulated in breast cancer patients compared with healthy controls. Of these, 10 miRNAs were previously identified as dysregulated in breast cancer; 14 miRNAs remained significant after P-values were adjusted by both correction methods. Principal component analysis and hierarchical clustering of these miRNAs separated patients from controls. Furthermore, the 3-miRNA signature (miR-199a, miR-29c, and miR-424) with the highest diagnostic accuracy for distinguishing breast cancer patients from controls by ROC curve analysis (AUC = 0.888) was successfully confirmed in the validation set (AUC = 0.901). Our data demonstrate that the SdM-RT-PCR assay is an effective breast cancer profiling method that utilizes very small volumes and is compatible with Biobank. Furthermore, the identified 3-miRNA signature is a promising circulating biomarker for breast cancer diagnosis.

语种英语
所属项目编号2012AA022501 ; 81170097 ; 81373070 ; BK2011381
资助者National High-Tech R&amp ; D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Jiangsu ; 985 Project of Peking University ; National High-Tech R&amp ; D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Jiangsu ; 985 Project of Peking University
WOS记录号WOS:000365188600020
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62890
专题北京大学临床肿瘤学院_乳腺癌预防治疗中心
作者单位1.Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Breast Ctr, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China
3.Suzhou Ribo Life Sci Co Ltd, Suzhou, Jiangsu, Peoples R China
4.Beijing Fuwai Hosp, Natl Ctr Cardiovasc Dis, Div Prevent & Community Hlth, Beijing, Peoples R China
5.Kunshan Ind Technol Res Inst, siRNA Biotechnol Res Inst, Suzhou, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Lei,Xu, Ye,Jin, Xingyu,et al. A circulating miRNA signature as a diagnostic biomarker for non-invasive early detection of breast cancer[J]. BREAST CANCER RESEARCH AND TREATMENT,2015,154(2):423-434.
APA Zhang, Lei.,Xu, Ye.,Jin, Xingyu.,Wang, Zengwu.,Wu, Yidi.,...&Liang, Zicai.(2015).A circulating miRNA signature as a diagnostic biomarker for non-invasive early detection of breast cancer.BREAST CANCER RESEARCH AND TREATMENT,154(2),423-434.
MLA Zhang, Lei,et al."A circulating miRNA signature as a diagnostic biomarker for non-invasive early detection of breast cancer".BREAST CANCER RESEARCH AND TREATMENT 154.2(2015):423-434.
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