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The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation
Lv, Longwei1; Liu, Yunsong1; Zhang, Ping1; Zhang, Xiao1; Liu, Jianzhang1; Chen, Tong1; Su, Penglei2; Li, Hongyi2; Zhou, Yongsheng1,3
关键词Titanium Nanotubes Adipose-derived stem cells Osteogenic differentiation Bone tissue engineering Epigenetics
刊名BIOMATERIALS
2015
DOI10.1016/j.biomaterials.2014.11.002
39页:193-205
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
资助者National Natural Science Foundation of China ; Ministry of Education ; Peking University 985 Program ; PKU School of Stomatology ; National Health and Family Planning Commission of China ; National Natural Science Foundation of China ; Ministry of Education ; Peking University 985 Program ; PKU School of Stomatology ; National Health and Family Planning Commission of China
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]BONE MORPHOGENETIC PROTEIN-2 ; STROMAL CELLS ; GENE-EXPRESSION ; MARROW ; REGENERATION ; TITANIUM ; ADHESION ; TISSUE ; PROLIFERATION ; OSSEOINTEGRATION
英文摘要

Nanostructured materials can direct stem cell lineage commitment solely by their various, but controllable, geometric cues, which would be very important for their future application in bone tissue engineering and bone regeneration. However, the mechanisms by which nano-geometric cues dictate the osteogenic differentiation of stem cells remain unclear. Epigenetics is central to cellular differentiation, a process that regulates heritable and long-lasting alterations in gene expression without changing the DNA sequence. Here, we explored the varied osteogenic behaviors of human adipose-derived stem cells (hASCs) on titanium dioxide (TiO2) nanotube arrays of different diameters. Both in vitro and in vivo studies demonstrated that the nanoscale geometry influenced cellular differentiation and TiO2 nanotubes with a diameter of 70 nm was the optimal dimension for the osteogenic differentiation of hASCs. Moreover, we observed that TiO2 nanotubes promoted the osteogenic differentiation of hASCs by upregulating methylation level of histone H3 at lysine 4 (H3K4) in the promoter regions of osteogenic genes Runx2 and osteocalcin, by inhibiting demethylase retinoblastoma binding protein 2 (RBP2). These results revealed, for the first time, the epigenetic mechanism by which nanotopography directs stem cell fate. (C) 2014 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号81070809 ; 81371118 ; 51002004 ; NCET-11-0026 ; PKUSS20120109
资助者National Natural Science Foundation of China ; Ministry of Education ; Peking University 985 Program ; PKU School of Stomatology ; National Health and Family Planning Commission of China ; National Natural Science Foundation of China ; Ministry of Education ; Peking University 985 Program ; PKU School of Stomatology ; National Health and Family Planning Commission of China
WOS记录号WOS:000347760600021
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62892
专题北京大学口腔医学院_口腔修复科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
2.Beijing Univ Technol, Photoelectrochem Res Grp, Sch Mat Sci & Engn, Beijing 100124, Peoples R China
3.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Lv, Longwei,Liu, Yunsong,Zhang, Ping,et al. The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation[J]. BIOMATERIALS,2015,39:193-205.
APA Lv, Longwei.,Liu, Yunsong.,Zhang, Ping.,Zhang, Xiao.,Liu, Jianzhang.,...&Zhou, Yongsheng.(2015).The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation.BIOMATERIALS,39,193-205.
MLA Lv, Longwei,et al."The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation".BIOMATERIALS 39(2015):193-205.
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