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学科主题: 临床医学
题名:
Human Papillomavirus Messenger RNA Assay for Cervical Cancer Screening The Shenzhen Cervical Cancer Screening Trial I
作者: Wu, Ruifang1; Belinson, Suzanne Elizabeth2; Du, Hui1; Na, Wulan1; Qu, Xinfeng3; Wu, Ruosong3; Liu, Ying1; Wang, Chun1; Zhou, Yanqiu1; Zhang, Lijie1; Belinson, Jerome L.2,4
关键词: HPV ; APTIMA ; Screening ; Cervical cancer
刊名: INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
发表日期: 2010-11-01
DOI: 10.1111/IGC.0b013e3181f29547
卷: 20, 期:8, 页:1411-1414
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: PERFORMANCE ; DETECT
英文摘要:

Introduction: Testing for high-risk types of human papillomavirus (HPV) has been consistently more sensitive than cervical cytology for high-grade precancers and cancers of the cervix (cervical intraepithelial neoplasia grade 2 or higher) but less specific. New assays are being developed to improve on the overall accuracy of molecular testing. The Gen-Probe APTIMA HPV assay (AHPV) is a multiplex assay that qualitatively detects 14 HPV types in a single tube. Because the AHPV targets HPV-E6/E7 messenger RNA transcripts, it should theoretically have a greater specificity than HPV assays that detect HPV DNA. The objective of this study was to compare the sensitivity and the specificity of the Gen-Probe AHPV with those of the Qiagen Hybrid Capture 2 assay (HC2) and liquid-based cytologic examination for cervical cancer screening.

Methods: A total of 2098 unscreened or poorly screened women 25 to 59 years of age were recruited in the city of Shenzhen, China. Two cervical specimens were collected: 1 in SurePath liquid for cytologic examination and 1 in PreservCyt for HPV testing by HC2 and the AHPV. The testing was performed by blinded technicians according to the manufacturer′s instructions. Women who had atypical squamous cells of undetermined significance or worse cytologic diagnosis and/or were HPV positive by either assay were asked to return for colposcopy and biopsy.

Results: Overall, 2095 women had complete data. Overall, 16.5% of the women were positive on HC2, 10.1% were positive on the AHPV, 5.45% had atypical squamous cells of undetermined significance or greater on cytologic examination, and 1.4% had histologically confirmed cervical disease: cervical intraepithelial neoplasia grade 2 or higher. The sensitivity values of liquid-based cytologic examination, HC2, and the AHPV were 66.7%, 88.9%, and 100%, respectively. The specificity values were 95.5%, 84.5%, and 91.2%, respectively. The AHPV was significantly more accurate by receiver operating characteristic curve comparison (P = 0.005).

Conclusions: The low false-positive rate (high specificity) and the high sensitivity of the AHPV makes this assay suitable for use as a primary assay for detecting cervical disease in a screening setting.

语种: 英语
项目资助者: Peking University Shenzhen Hospital, Shenzhen, China ; Royal Ladies Gynecology Clinic, Shenzhen, China ; Preventive Oncology International, Inc, Cleveland Heights, OH ; Gen-Probe, Inc, San Diego, CA ; Qiagen, Inc, Gaithersburg, MD
WOS记录号: WOS:000283850500023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62894
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China
2.Prevent Oncol Int Inc, Cleveland Hts, OH 44118 USA
3.Royal Ladies Gynecol Clin, Shenzhen, Peoples R China
4.Cleveland Clin, Cleveland, OH 44106 USA

Recommended Citation:
Wu, Ruifang,Belinson, Suzanne Elizabeth,Du, Hui,et al. Human Papillomavirus Messenger RNA Assay for Cervical Cancer Screening The Shenzhen Cervical Cancer Screening Trial I[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2010,20(8):1411-1414.
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