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Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro
Wang, Peng; Zou, Xiao-Min; Huang, Jian; Zhang, Tian-Lan; Wang, Kui
关键词differentiation gadolinium migration osteoclast prostate cancer
刊名CELL BIOLOGY INTERNATIONAL
2011-11-01
DOI10.1042/CBI20100870
35期:11页:1159-1167
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]SIGNALING PATHWAY ; BONE METASTASES ; PROTEIN ; TUMOR ; ACTIVATION ; LANTHANUM ; MICROENVIRONMENT ; OSTEOPROTEGERIN ; ANGIOGENESIS ; PROGRESSION
英文摘要

This study examined whether Gd (gadolinium) could suppress prostate cancer cell migration and prostate cancer cell-induced osteoclast differentiation. MU [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] and colony forming assay showed that GdCl(3) treatment inhibited both cell viability and colony forming ability in PC3 cells more significantly than that in DU145 cells. Annexin/PI (propidium iodide) staining showed an increase in apoptotic death of PC3 cells in the presence of GdCl(3). Wound healing and adhesion assay indicated that GdCl(3) suppressed PC3 cell migration. Western-blot analysis demonstrated that GdCl(3) treatment inhibited phosphorylation of ERK (extracellular-signal-regulated kinase) and p38 MAPK (mitogen-activated protein kinase). Pretreatment with PTx (pertussis toxin), a Gi protein inhibitor, conferred resistance to GdCl(3)-induced colony formation, ERK and p38 phosphorylation in PC3 cells. Moreover, GdCl(3) inhibited PC3 cell-induced osteoclast differentiation. RT-PCR (reverse transcription-PCR) indicated that GdCl(3) decreased the expression of RANKL (receptor activator of nuclear factor-kappa B ligand) in PC3 cells, whereas it increased the expression of OPG (osteoprotegerin) in PC3 and DU145 cells. In conclusion, the present study indicated that GdCl(3) inhibited PC3 cell migration mediated by the inactivation of both ERK and p38 MAPK pathways via PTx-sensitive G proteins, and also suppressed PC3 cell-induced osteoclast differentiation via regulating the mRNA expression of OPG and RANKL.

语种英语
WOS记录号WOS:000297086100012
项目编号200800011058 ; 2007CB516806
资助机构Specialized Research Fund for the Doctoral Program of Higher Education ; National Basic Research Program of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62918
专题北京大学药学院
北京大学药学院_化学生物学系
作者单位Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wang, Peng,Zou, Xiao-Min,Huang, Jian,et al. Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro[J]. CELL BIOLOGY INTERNATIONAL,2011,35(11):1159-1167.
APA Wang, Peng,Zou, Xiao-Min,Huang, Jian,Zhang, Tian-Lan,&Wang, Kui.(2011).Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro.CELL BIOLOGY INTERNATIONAL,35(11),1159-1167.
MLA Wang, Peng,et al."Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro".CELL BIOLOGY INTERNATIONAL 35.11(2011):1159-1167.
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