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学科主题: 药学
题名:
Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro
作者: Wang, Peng; Zou, Xiao-Min; Huang, Jian; Zhang, Tian-Lan; Wang, Kui
关键词: differentiation ; gadolinium ; migration ; osteoclast ; prostate cancer
刊名: CELL BIOLOGY INTERNATIONAL
发表日期: 2011-11-01
DOI: 10.1042/CBI20100870
卷: 35, 期:11, 页:1159-1167
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: SIGNALING PATHWAY ; BONE METASTASES ; PROTEIN ; TUMOR ; ACTIVATION ; LANTHANUM ; MICROENVIRONMENT ; OSTEOPROTEGERIN ; ANGIOGENESIS ; PROGRESSION
英文摘要:

This study examined whether Gd (gadolinium) could suppress prostate cancer cell migration and prostate cancer cell-induced osteoclast differentiation. MU [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] and colony forming assay showed that GdCl(3) treatment inhibited both cell viability and colony forming ability in PC3 cells more significantly than that in DU145 cells. Annexin/PI (propidium iodide) staining showed an increase in apoptotic death of PC3 cells in the presence of GdCl(3). Wound healing and adhesion assay indicated that GdCl(3) suppressed PC3 cell migration. Western-blot analysis demonstrated that GdCl(3) treatment inhibited phosphorylation of ERK (extracellular-signal-regulated kinase) and p38 MAPK (mitogen-activated protein kinase). Pretreatment with PTx (pertussis toxin), a Gi protein inhibitor, conferred resistance to GdCl(3)-induced colony formation, ERK and p38 phosphorylation in PC3 cells. Moreover, GdCl(3) inhibited PC3 cell-induced osteoclast differentiation. RT-PCR (reverse transcription-PCR) indicated that GdCl(3) decreased the expression of RANKL (receptor activator of nuclear factor-kappa B ligand) in PC3 cells, whereas it increased the expression of OPG (osteoprotegerin) in PC3 and DU145 cells. In conclusion, the present study indicated that GdCl(3) inhibited PC3 cell migration mediated by the inactivation of both ERK and p38 MAPK pathways via PTx-sensitive G proteins, and also suppressed PC3 cell-induced osteoclast differentiation via regulating the mRNA expression of OPG and RANKL.

语种: 英语
所属项目编号: 200800011058 ; 2007CB516806
项目资助者: Specialized Research Fund for the Doctoral Program of Higher Education ; National Basic Research Program of China
WOS记录号: WOS:000297086100012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62918
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Peng,Zou, Xiao-Min,Huang, Jian,et al. Gadolinium inhibits prostate cancer PC3 cell migration and suppresses osteoclast differentiation in vitro[J]. CELL BIOLOGY INTERNATIONAL,2011,35(11):1159-1167.
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