北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Composition and Interactions of Hepatitis B Virus Quasispecies Defined the Virological Response During Telbivudine Therapy
作者: Zhou, Bin1; Dong, Hui2; He, Yungang3; Sun, Jian1; Jin, Weirong2,4; Xie, Qing5; Fan, Rong1; Wang, Minxian3; Li, Ran3; Chen, Yangyi2; Xie, Shaoqing4; Shen, Yan4; Huang, Xin3; Wang, Shengyue2; Lu, Fengming6; Jia, Jidong7; Zhuang, Hui6; Locarnini, Stephen8; Zhao, Guo-Ping2,3,9,10,11,12,13; Jin, Li3,10,11,12,13; Hou, Jinlin1,14
刊名: SCIENTIFIC REPORTS
发表日期: 2015-11-24
DOI: 10.1038/srep17123
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: NAIVE PATIENTS ; DRUG-RESISTANCE ; MUTANTS
英文摘要:

Reverse transcriptase (RT) mutations contribute to hepatitis B virus resistance during antiviral therapy with nucleos(t)ide analogs. However, the composition of the RT quasispecies and their interactions during antiviral treatment have not yet been thoroughly defined. In this report, 10 patients from each of 3 different virological response groups, i.e., complete virological response, partial virological response and virological breakthrough, were selected from a multicenter trial of Telbivudine treatment. Variations in the drug resistance-related critical RT regions in 107 serial serum samples from the 30 patients were examined by ultra-deep sequencing. A total of 496,577 sequence reads were obtained, with an average sequencing coverage of 4,641X per sample. The phylogenies of the quasispecies revealed the independent origins of two critical quasispecies, i.e., the rtA181T and rtM204I mutants. Data analyses and theoretical modeling showed a cooperative-competitive interplay among the quasispecies. In particular, rtM204I mutants compete against other quasispecies, which eventually leads to virological breakthrough. However, in the absence of rtM204I mutants, synergistic growth of the drug-resistant rtA181T mutants with the wild-type quasispecies could drive

语种: 英语
所属项目编号: 2012ZX10002003 ; 2013ZX10004104-002 ; 12ZR1421200 ; 91331107 ; 91331109 ; 31171279
项目资助者: National Science and Technology Major Project ; key Clinical Specialty Discipline Construction Program, Shanghai Commission for Science and Technology ; National Natural Science Foundation of China
WOS记录号: WOS:000365198100002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62926
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Southern Med Univ, Nanfang Hosp, Dept Infect Dis, Guangdong Prov Key Lab Viral Hepatitis Res,State, Guangzhou, Peoples R China
2.Chinese Natl Human Genome Ctr, Shanghai MOST Key Lab Hlth & Dis Gen, Shanghai, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol,CAS Key Lab Computation, CAS Key Lab Synthet Biol,CAS MPG Partner Inst Com, Shanghai, Peoples R China
4.Shanghai Shenyou Biotechnol Co Ltd, Shanghai, Peoples R China
5.Victorian Infect Dis Reference Lab, Victoria, Australia
6.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Infect Dis, Shanghai 200030, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Ctr Infect Dis,Dept Microbiol, Beijing 100871, Peoples R China
8.Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, Beijing, Peoples R China
9.Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
10.Fudan Univ, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
11.Fudan Univ, Minist Educ, Key Lab Contemporary Anthropol, Sch Life Sci, Shanghai 200433, Peoples R China
12.Fudan Univ, Inst Biomed Sci, Key Lab Med Mol Virol, Ministries Educ & Hlth,Shanghai Med Coll, Shanghai 200433, Peoples R China
13.Fudan Univ, Dept Microbiol, Sch Life Sci, Shanghai 200433, Peoples R China
14.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310003, Zhejiang, Peoples R China

Recommended Citation:
Zhou, Bin,Dong, Hui,He, Yungang,et al. Composition and Interactions of Hepatitis B Virus Quasispecies Defined the Virological Response During Telbivudine Therapy[J]. SCIENTIFIC REPORTS,2015,5.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, Bin]'s Articles
[Dong, Hui]'s Articles
[He, Yungang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, Bin]‘s Articles
[Dong, Hui]‘s Articles
[He, Yungang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace