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学科主题: 口腔医学
题名:
Snail regulates the motility of oral cancer cells via RhoA/Cdc42/p-ERM pathway
作者: Li, Yao-yin; Zhou, Chuan-Xiang; Gao, Yan
关键词: Cell motility ; Oral squamous cell carcinoma ; Snail ; RhoA/Cdc42/p-ERM pathway ; Epithelial-to-mesenchymal transition ; Metastasis
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2014-09-26
DOI: 10.1016/j.bbrc.2014.08.110
卷: 452, 期:3, 页:490-496
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: RHO GTPASES ; INVASION ; MIGRATION ; FAMILY ; CYTOSKELETON ; METASTASIS ; EXPRESSION ; ADHESION ; MATRIX ; CDC42
英文摘要:

The transcriptional factor Snail has been reported to possess properties related to cancer progression; however, the mechanism for it is not fully understood. Our data showed that Snail knockdown by small interfering RNA in two OSCC cell lines, WSU-HN6 and CAL27, significantly inhibited cell migration and invasion which also resulted in decreased cell motility, such as impaired cell spreading on type I collagen substrate, reduced filopodia, and premature assembly of stress fibers. In addition, Snail-silencing decreased Cdc42 activity but increased RhoA activity, accompanied by the downregulation in both p-ERM expression and cell motility. Meanwhile, endogenous p-ERM was found specifically co-precipitated with activated Cdc42, but not RhoA, and this co-association was decreased by Snail-silencing. The small molecule inhibitors of Rho-associated kinase (Y27632) markedly enhanced Cdc42 activity and the association of p-ERM with activated Cdc42, increasing cell motility remarkably. Using immunohistochemistry, Snail and p-ERM overexpressions were found in OSCC tissues correlated with nodal metastasis and shorter survival. Taken together, these results demonstrate that Snail regulates cell motility through RhoA/Cdc42/p-ERM pathway and may serve as a biomarker to predict prognosis for OSCC patients. Although RhoA and Cdc42 are concurrently regulated downstream of Snail, there is a direct interplay between them, which indicates RhoA has to be inactivated at some point in cell motility cycle. (C) 2014 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30973338
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000343023900030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62933
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

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作者单位: Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China

Recommended Citation:
Li, Yao-yin,Zhou, Chuan-Xiang,Gao, Yan. Snail regulates the motility of oral cancer cells via RhoA/Cdc42/p-ERM pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,452(3):490-496.
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