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Snail regulates the motility of oral cancer cells via RhoA/Cdc42/p-ERM pathway
Li, Yao-yin; Zhou, Chuan-Xiang; Gao, Yan
关键词Cell motility Oral squamous cell carcinoma Snail RhoA/Cdc42/p-ERM pathway Epithelial-to-mesenchymal transition Metastasis
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2014-09-26
DOI10.1016/j.bbrc.2014.08.110
452期:3页:490-496
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]RHO GTPASES ; INVASION ; MIGRATION ; FAMILY ; CYTOSKELETON ; METASTASIS ; EXPRESSION ; ADHESION ; MATRIX ; CDC42
英文摘要

The transcriptional factor Snail has been reported to possess properties related to cancer progression; however, the mechanism for it is not fully understood. Our data showed that Snail knockdown by small interfering RNA in two OSCC cell lines, WSU-HN6 and CAL27, significantly inhibited cell migration and invasion which also resulted in decreased cell motility, such as impaired cell spreading on type I collagen substrate, reduced filopodia, and premature assembly of stress fibers. In addition, Snail-silencing decreased Cdc42 activity but increased RhoA activity, accompanied by the downregulation in both p-ERM expression and cell motility. Meanwhile, endogenous p-ERM was found specifically co-precipitated with activated Cdc42, but not RhoA, and this co-association was decreased by Snail-silencing. The small molecule inhibitors of Rho-associated kinase (Y27632) markedly enhanced Cdc42 activity and the association of p-ERM with activated Cdc42, increasing cell motility remarkably. Using immunohistochemistry, Snail and p-ERM overexpressions were found in OSCC tissues correlated with nodal metastasis and shorter survival. Taken together, these results demonstrate that Snail regulates cell motility through RhoA/Cdc42/p-ERM pathway and may serve as a biomarker to predict prognosis for OSCC patients. Although RhoA and Cdc42 are concurrently regulated downstream of Snail, there is a direct interplay between them, which indicates RhoA has to be inactivated at some point in cell motility cycle. (C) 2014 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000343023900030
项目编号30973338
资助机构National Nature Science Foundation of China
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62933
专题北京大学口腔医学院_口腔病理科
作者单位Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
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Li, Yao-yin,Zhou, Chuan-Xiang,Gao, Yan. Snail regulates the motility of oral cancer cells via RhoA/Cdc42/p-ERM pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,452(3):490-496.
APA Li, Yao-yin,Zhou, Chuan-Xiang,&Gao, Yan.(2014).Snail regulates the motility of oral cancer cells via RhoA/Cdc42/p-ERM pathway.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,452(3),490-496.
MLA Li, Yao-yin,et al."Snail regulates the motility of oral cancer cells via RhoA/Cdc42/p-ERM pathway".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 452.3(2014):490-496.
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