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学科主题: 基础医学
题名:
Tetraphenylethylene Conjugated with a Specific Peptide as a Fluorescence Turn-On Bioprobe for the Highly Specific Detection and Tracing of Tumor Markers in Live Cancer Cells
作者: Huang, Yanyan1; Hu, Fang1; Zhao, Rui1; Zhang, Guanxin1; Yang, Hua2; Zhang, Deqing1
关键词: aggregation-induced emission ; cancer ; fluorescent probes ; peptides ; tetraphenylethylene
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2014-01-03
DOI: 10.1002/chem.201303679
卷: 20, 期:1, 页:158-164
收录类别: SCI ; IC
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: AGGREGATION-INDUCED EMISSION ; METAL-ORGANIC FRAMEWORKS ; HEPATOCELLULAR-CARCINOMA ; IN-VIVO ; LAPTM4B ; SILOLE ; ASSAY ; ACID ; PH ; LUMINESCENCE
英文摘要:

Smart molecular probes and flexible methods are attracting remarkable interest for the visualization of cancer-related biological and chemical events. In this work, a new fluorescence turn-on probe with dual-recognition characteristics for the specific imaging of cancer cells is reported. This new bioprobe is rationally designed by linking tetraphenylethylene (TPE), an aggregation-induced emission (AIE) fluorophore, with the small peptide IHGHHIISVG (referred to as AP2H), a targeting ligand to the broad-spectrum cancer-related protein LAPTM4B. The binding of the probe TPE-AP2H with the target, both in solution and at the cellular level, switches on the fluorescence of TPE because of the inhibition of internal rotations within the TPE framework. Accordingly, this bioprobe allows the real-time monitoring and subcellular localization of LAPTM4B in cancer cells, with a very high target-to-background ratio for the imaging. Furthermore, brighter fluorescence images are detected after incubation of TPE-AP2H with tumor cells at lower pH values. Thus, this new bioprobe is more advantageous because it can simultaneously target the LAPTM4B protein and sense the characteristic low-pH environment of tumor cells. In addition, TPE-AP2H displays high photostability and low cytotoxicity. Therefore, this new bioprobe is promising for the more accurate and reliable imaging of tumor markers in live cancer cells.

语种: 英语
所属项目编号: 21105105 ; 21135006 ; 21321003 ; 21190032 ; 21075126
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Chinese Academy of Sciences
WOS记录号: WOS:000328714700020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62947
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Labs Analyt Chem Living Biosyst & Organ S, Inst Chem, Beijing 100190, Peoples R China
2.Peking Univ, Dept Cell Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Yanyan,Hu, Fang,Zhao, Rui,et al. Tetraphenylethylene Conjugated with a Specific Peptide as a Fluorescence Turn-On Bioprobe for the Highly Specific Detection and Tracing of Tumor Markers in Live Cancer Cells[J]. CHEMISTRY-A EUROPEAN JOURNAL,2014,20(1):158-164.
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