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学科主题: 临床医学
题名:
Possible mechanism for the regulation of glucose on proliferation, inhibition and apoptosis of colon cancer cells induced by sodium butyrate
作者: He, Lei; Li, Xi; Luo, He-Sheng; Rong, Han; Cai, Jia
关键词: sodium butyrate ; glucose ; glucose transporter 1 ; monocarboxylate transporter 1
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2007-08-07
卷: 13, 期:29, 页:4015-4018
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: MONOCARBOXYLATE TRANSPORTER ; EXPRESSION ; DEPRIVATION ; SENSITIVITY ; METABOLISM ; SURVIVAL ; GROWTH
英文摘要:

AIM: To study the effect of glucose on sodium butyrate-induced proliferation inhibition and apoptosis in HT-29 cell line, and explored its possible mechanisms.

METHODS: HT-29 cells were grown in RPMI-1640 medium supplemented with 10% fetal calf serum, and were allowed to adhere for 24 h, and then replaced with experimental medium. Cell survival rates were detected by MTT assay. Apoptosis was detected by TUNEL assay. Glucose transport protein 1 (GLUT1) and monocarboxylate transporter I (MCT1) mRNA expression was detected by RT-PCR.

RESULTS: Low concentration of glucose induced apoptosis and regulated proliferation in HT-29 cell line, and glucose can obviously inhibit the effect of proliferation inhibition and apoptosis induced by sodium butyrate. Glucose also down-regulated the expression of (MCT1) mRNA (0.28 +/- 0.07 vs 0.19 +/- 0.10, P < 0.05), and decreased the expression of GLUTlmRNA slightly (0.18 +/- 0.04 vs 0.13 +/- 0.03, P < 0.05).

CONCLUSION: Glucose can regulate the effect of proliferation inhibition and apoptosis induced by sodium butyrate and this influence may be associated with the intracellular concentration of glucose and sodium butyrate. (c) 2007 WJG. All rights reserved.

语种: 英语
WOS记录号: WOS:000248546500019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62955
Appears in Collections:北京大学深圳医院_消化内科_期刊论文

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作者单位: 1.Wuhan Univ, Renmin Hosp, Dept Gastroenterol, Wuhan 430060, Hubei, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Gastroenterol, Shenzhen 518036, Guangdong, Peoples R China
3.Wuhan Univ, Renmin Hosp, Dept Psychiat, Wuhan 430060, Hubei, Peoples R China

Recommended Citation:
He, Lei,Li, Xi,Luo, He-Sheng,et al. Possible mechanism for the regulation of glucose on proliferation, inhibition and apoptosis of colon cancer cells induced by sodium butyrate[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2007,13(29):4015-4018.
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