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学科主题: 基础医学
题名:
Seasonal Changes in Immunoreactivity of Vascular Endothelial Factor and its Receptors VEGFR1 and VEGFR2 in the Uterus of Wild Ground Squirrels (Citellus dauricus Brandt)
作者: Weng, Jiaju2; Li, Ben1; Sheng, Xia1; Zhang, Haolin1; Hu, Xiao1,3; Zhou, Jiao1; Xu, Meiyu1; Weng, Qiang1,4; Watanabe, Gen4,5; Taya, Kazuyoshi4,5
关键词: Uterus ; VEGF ; VEGFR1 ; VEGFR2 ; Wild ground squirrel
刊名: JOURNAL OF REPRODUCTION AND DEVELOPMENT
发表日期: 2012-10-01
卷: 58, 期:5, 页:537-543
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Agriculture, Dairy & Animal Science ; Reproductive Biology
研究领域[WOS]: Agriculture ; Reproductive Biology
关键词[WOS]: GROWTH-FACTOR VEGF ; HUMAN ENDOMETRIUM ; FACTOR EXPRESSION ; RAT UTERUS ; MENSTRUAL-CYCLE ; EARLY-PREGNANCY ; ESTROUS-CYCLE ; CELL-PROLIFERATION ; RHESUS-MONKEY ; ANGIOGENESIS
英文摘要:

In this study, we investigated the immunoreactivity of vascular endothelial growth factor (VEGF) and its receptors fit-1 (VEGFR I) and the kinase domain receptor (KDR/Flk-1, VEGFR2) in the uteri of the wild ground squirrels during the estrous period, early pregnancy and nonbreeding period. Cellular localizations of VEGF, VEGFR 1 and VEGFR2 were detected by immunohistochemistry, and total proteins were extracted from uterine tissue in the estrous period, early pregnancy and nonbreeding period for Western blotting analysis. In addition, plasma estradiol-17 beta and progesterone concentrations were measured by radioimmunoassay. Stronger positive staining of VEGF was found in luminal epithelial cells and glandular cells, and its receptors (VEGFR1 and VEGFR2) were observed in stromal cells in the estrous period and early pregnancy compared with the nonbreeding period. The protein levels of VEGF, VEGFR1 and VEGFR2 were significantly higher in the estrous period and early pregnancy as compared with the nonbreeding period. Besides, plasma estradio1-17 beta and progesterone concentrations were higher in the estrous period and early pregnancy than in the nonbreeding period, suggesting that the immunoreactivities of VEGF, VEGFR1 and VEGFR2 were correlated with changes in plasma estradiol-17 beta and progesterone concentrations. These results suggested that VEGF and its receptors may be involved in the regulation of seasonal changes in the uterine functions of wild female ground squirrels.

语种: 英语
所属项目编号: IRT0607
项目资助者: Grant-in-Aid from the Program for Changjiang Scholars and Innovative Research Team in Universities of China
WOS记录号: WOS:000310716800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62960
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
3.Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan
4.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China
5.Tokyo Univ Agr & Technol, Fac Agr, Cooperat Dept Vet Med, Lab Vet Physiol, Tokyo 1838509, Japan

Recommended Citation:
Weng, Jiaju,Li, Ben,Sheng, Xia,et al. Seasonal Changes in Immunoreactivity of Vascular Endothelial Factor and its Receptors VEGFR1 and VEGFR2 in the Uterus of Wild Ground Squirrels (Citellus dauricus Brandt)[J]. JOURNAL OF REPRODUCTION AND DEVELOPMENT,2012,58(5):537-543.
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