Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays

doi:10.1080/09540100903443709

IR@PKUHSC > 北京大学第三临床医学院 > 皮肤科

学科主题	临床医学
	Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays
	Lou, F.1; Cong, Y. J.2; Ibrahim, Salam A.3; Ren, F. Z.1; Li, L. F.4
关键词	allergenicity antigenicity cow milk substitute
刊名	FOOD AND AGRICULTURAL IMMUNOLOGY
	2010
DOI	10.1080/09540100903443709
卷	21 期:2 页:119-130
收录类别	SCI
文章类型	Article
WOS标题词	Science & Technology
类目[WOS]	Chemistry, Applied ; Food Science & Technology ; Immunology ; Toxicology
研究领域[WOS]	Chemistry ; Food Science & Technology ; Immunology ; Toxicology
关键词[WOS]	IN-VITRO ; PROTEINS ; IGE ; HYDROLYSIS ; ARA-H-1 ; POTENCY
英文摘要	We conducted enzyme-linked immunosorbent assay (ELISA) and basophil-activation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks.
语种	英语
WOS记录号	WOS:000277487500004
资助机构	National Science and Technology Supporting Item (China)
引用统计
文献类型	期刊论文
条目标识符	http://ir.bjmu.edu.cn/handle/400002259/62963
专题	北京大学第三临床医学院_皮肤科
作者单位	1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China 2.Beijing Technol & Business Univ, Coll Chem & Environm Engn, Beijing 100048, Peoples R China 3.N Carolina Agr & Tech State Univ, Food Microbiol & Biotechnol Lab, Greensboro, NC 27411 USA 4.Peking Univ, Hosp 3, Dept Dermatol, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Lou, F.,Cong, Y. J.,Ibrahim, Salam A.,et al. Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays[J]. FOOD AND AGRICULTURAL IMMUNOLOGY,2010,21(2):119-130.
APA	Lou, F.,Cong, Y. J.,Ibrahim, Salam A.,Ren, F. Z.,&Li, L. F..(2010).Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays.FOOD AND AGRICULTURAL IMMUNOLOGY,21(2),119-130.
MLA	Lou, F.,et al."Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays".FOOD AND AGRICULTURAL IMMUNOLOGY 21.2(2010):119-130.