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Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays
Lou, F.1; Cong, Y. J.2; Ibrahim, Salam A.3; Ren, F. Z.1; Li, L. F.4
关键词allergenicity antigenicity cow milk substitute
刊名FOOD AND AGRICULTURAL IMMUNOLOGY
2010
DOI10.1080/09540100903443709
21期:2页:119-130
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Applied ; Food Science & Technology ; Immunology ; Toxicology
研究领域[WOS]Chemistry ; Food Science & Technology ; Immunology ; Toxicology
关键词[WOS]IN-VITRO ; PROTEINS ; IGE ; HYDROLYSIS ; ARA-H-1 ; POTENCY
英文摘要

We conducted enzyme-linked immunosorbent assay (ELISA) and basophil-activation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks.

语种英语
WOS记录号WOS:000277487500004
资助机构National Science and Technology Supporting Item (China)
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62963
专题北京大学第三临床医学院_皮肤科
作者单位1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
2.Beijing Technol & Business Univ, Coll Chem & Environm Engn, Beijing 100048, Peoples R China
3.N Carolina Agr & Tech State Univ, Food Microbiol & Biotechnol Lab, Greensboro, NC 27411 USA
4.Peking Univ, Hosp 3, Dept Dermatol, Beijing 100083, Peoples R China
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GB/T 7714
Lou, F.,Cong, Y. J.,Ibrahim, Salam A.,et al. Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays[J]. FOOD AND AGRICULTURAL IMMUNOLOGY,2010,21(2):119-130.
APA Lou, F.,Cong, Y. J.,Ibrahim, Salam A.,Ren, F. Z.,&Li, L. F..(2010).Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays.FOOD AND AGRICULTURAL IMMUNOLOGY,21(2),119-130.
MLA Lou, F.,et al."Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays".FOOD AND AGRICULTURAL IMMUNOLOGY 21.2(2010):119-130.
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