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学科主题: 临床医学
题名:
A Novel Protein Is Lower Expressed in Renal Cell Carcinoma
作者: Guan, Ruili1,2,3,4; Xu, Yongde1; Lei, Hongen1; Gao, Zhezhu1; Xin, Zhongcheng1; Guo, Yinglu2,3,4
关键词: engrailed-2 ; renal cell carcinoma ; tissue biomarker
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2014-05-01
DOI: 10.3390/ijms15057398
卷: 15, 期:5, 页:7398-7408
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: CARBONIC-ANHYDRASE-IX ; PROSTATE-CANCER ; ENGRAILED-2 EN2 ; BIOMARKER
英文摘要:

Engrailed-2 (EN2) has been identified as a candidate oncogene in breast cancer and prostate cancer. It is usually recognized as a mainly nuclear staining in the cells. However, recent studies showed a cytoplasmic staining occurred in prostate cancer, bladder cancer and clear cell renal cell carcinoma. The inconsistency makes us confused. To clarify the localization and expression of EN2 in renal cell carcinoma, anti-EN2 antibody (ab28731) and anti-EN2 antibody (MAB2600) were used for immunohistochemistry (IHC) respectively. Interestingly, we found that EN2 detected by ab28731 was mainly presented in cytoplasm while EN2 detected by MAB2600 was mainly presented in nucleus. To further investigate the different patterns observed above, lysates from full-length EN2 over expression in HEK293T cells were used to identify which antibody the EN2 molecule bound by western blot. Results showed ab28731 did not react with the lysates. For this reason, the novel specific protein detected by ab28731 was not the EN2 molecule and was named nonEN2. Then using the renal carcinoma tissue microarray and renal tissues, we found that the protein expression levels of nonEN2 in kidney tumor tissues was significantly lower than that in kidney normal tissues (p < 0.05), so was in renal cell lines. Taken together, nonEN2 is lower expressed and may play an important role in renal cell carcinoma.

语种: 英语
项目资助者: China Health &amp ; Medical Development Foundation
WOS记录号: WOS:000336841800019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62965
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
2.Natl Urol Canc Ctr, Beijing 100034, Peoples R China
3.Peking Univ, Androl Ctr, Hosp 1, Beijing 100034, Peoples R China
4.Peking Univ, Dept Urol, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Guan, Ruili,Xu, Yongde,Lei, Hongen,et al. A Novel Protein Is Lower Expressed in Renal Cell Carcinoma[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(5):7398-7408.
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