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A Novel Protein Is Lower Expressed in Renal Cell Carcinoma
Guan, Ruili1,2,3,4; Xu, Yongde1; Lei, Hongen1; Gao, Zhezhu1; Xin, Zhongcheng1; Guo, Yinglu2,3,4
关键词engrailed-2 renal cell carcinoma tissue biomarker
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2014-05-01
DOI10.3390/ijms15057398
15期:5页:7398-7408
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]CARBONIC-ANHYDRASE-IX ; PROSTATE-CANCER ; ENGRAILED-2 EN2 ; BIOMARKER
英文摘要

Engrailed-2 (EN2) has been identified as a candidate oncogene in breast cancer and prostate cancer. It is usually recognized as a mainly nuclear staining in the cells. However, recent studies showed a cytoplasmic staining occurred in prostate cancer, bladder cancer and clear cell renal cell carcinoma. The inconsistency makes us confused. To clarify the localization and expression of EN2 in renal cell carcinoma, anti-EN2 antibody (ab28731) and anti-EN2 antibody (MAB2600) were used for immunohistochemistry (IHC) respectively. Interestingly, we found that EN2 detected by ab28731 was mainly presented in cytoplasm while EN2 detected by MAB2600 was mainly presented in nucleus. To further investigate the different patterns observed above, lysates from full-length EN2 over expression in HEK293T cells were used to identify which antibody the EN2 molecule bound by western blot. Results showed ab28731 did not react with the lysates. For this reason, the novel specific protein detected by ab28731 was not the EN2 molecule and was named nonEN2. Then using the renal carcinoma tissue microarray and renal tissues, we found that the protein expression levels of nonEN2 in kidney tumor tissues was significantly lower than that in kidney normal tissues (p < 0.05), so was in renal cell lines. Taken together, nonEN2 is lower expressed and may play an important role in renal cell carcinoma.

语种英语
WOS记录号WOS:000336841800019
资助机构China Health &amp ; Medical Development Foundation
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62965
专题北京大学第一临床医学院_男科中心
北京大学第一临床医学院_北京大学泌尿外科研究所
作者单位1.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
2.Natl Urol Canc Ctr, Beijing 100034, Peoples R China
3.Peking Univ, Androl Ctr, Hosp 1, Beijing 100034, Peoples R China
4.Peking Univ, Dept Urol, Hosp 1, Beijing 100034, Peoples R China
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GB/T 7714
Guan, Ruili,Xu, Yongde,Lei, Hongen,et al. A Novel Protein Is Lower Expressed in Renal Cell Carcinoma[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(5):7398-7408.
APA Guan, Ruili,Xu, Yongde,Lei, Hongen,Gao, Zhezhu,Xin, Zhongcheng,&Guo, Yinglu.(2014).A Novel Protein Is Lower Expressed in Renal Cell Carcinoma.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,15(5),7398-7408.
MLA Guan, Ruili,et al."A Novel Protein Is Lower Expressed in Renal Cell Carcinoma".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 15.5(2014):7398-7408.
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