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学科主题: 基础医学
题名:
Synergistic effect of cytokines EPO, IL-3 and SCF on the proliferation, differentiation and apoptosis of erythroid progenitor cells
作者: Wang, Jinhua; Tang, Zhi-Yu; Ka, Weibo; Sun, DaGong; Yao, Weijuan; Wen, ZongYao; Chien, Shu
刊名: CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
发表日期: 2007
卷: 37, 期:4, 页:291-299
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: ERYTHROPOIETIN
英文摘要:

Erythroblasts were obtained from murine spleen. After cultured for 12 hr, the cells were divided into four groups with the use of the following cytokines in culture: EPO, EPO + SCF,EPO + IL-3, and EPO + IL-3 + SCF. Cell proliferation assay was done. Apoptosis rates were obtained by using a flow cytometer. Mitochondrial membrane potential (MMP) was assessed in flow cytometry (FCM) by labeling with rhodamine 123. Mitochondrial enzyme activity ( MEA) was evaluated with MTT colorimetric assay. The cells were labeled with Fluo- 3/Am Ester and Ca2+ concentration was measured. The expression of Bax mRNA and Bcl-2 mRNA was analyzed by RT-PCR. At same time, the expression of Bax and Bcl-2 was analyzed by western blotting. Our results showed that IL-3 and SCF have synergistic effects with EPO on the proliferation, differentiation and apoptosis of erythroid progenitors.

语种: 英语
WOS记录号: WOS:000250775100001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62978
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Biophys, Hermorheol Ctr, Beijing 100083, Peoples R China
2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

Recommended Citation:
Wang, Jinhua,Tang, Zhi-Yu,Ka, Weibo,et al. Synergistic effect of cytokines EPO, IL-3 and SCF on the proliferation, differentiation and apoptosis of erythroid progenitor cells[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2007,37(4):291-299.
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