IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
Synergistic effect of cytokines EPO, IL-3 and SCF on the proliferation, differentiation and apoptosis of erythroid progenitor cells
Wang, Jinhua; Tang, Zhi-Yu; Ka, Weibo; Sun, DaGong; Yao, Weijuan; Wen, ZongYao; Chien, Shu
刊名CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
2007
37期:4页:291-299
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology ; Peripheral Vascular Disease
研究领域[WOS]Hematology ; Cardiovascular System & Cardiology
关键词[WOS]ERYTHROPOIETIN
英文摘要

Erythroblasts were obtained from murine spleen. After cultured for 12 hr, the cells were divided into four groups with the use of the following cytokines in culture: EPO, EPO + SCF,EPO + IL-3, and EPO + IL-3 + SCF. Cell proliferation assay was done. Apoptosis rates were obtained by using a flow cytometer. Mitochondrial membrane potential (MMP) was assessed in flow cytometry (FCM) by labeling with rhodamine 123. Mitochondrial enzyme activity ( MEA) was evaluated with MTT colorimetric assay. The cells were labeled with Fluo- 3/Am Ester and Ca2+ concentration was measured. The expression of Bax mRNA and Bcl-2 mRNA was analyzed by RT-PCR. At same time, the expression of Bax and Bcl-2 was analyzed by western blotting. Our results showed that IL-3 and SCF have synergistic effects with EPO on the proliferation, differentiation and apoptosis of erythroid progenitors.

语种英语
WOS记录号WOS:000250775100001
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62978
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Biophys, Hermorheol Ctr, Beijing 100083, Peoples R China
2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
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GB/T 7714
Wang, Jinhua,Tang, Zhi-Yu,Ka, Weibo,et al. Synergistic effect of cytokines EPO, IL-3 and SCF on the proliferation, differentiation and apoptosis of erythroid progenitor cells[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2007,37(4):291-299.
APA Wang, Jinhua.,Tang, Zhi-Yu.,Ka, Weibo.,Sun, DaGong.,Yao, Weijuan.,...&Chien, Shu.(2007).Synergistic effect of cytokines EPO, IL-3 and SCF on the proliferation, differentiation and apoptosis of erythroid progenitor cells.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,37(4),291-299.
MLA Wang, Jinhua,et al."Synergistic effect of cytokines EPO, IL-3 and SCF on the proliferation, differentiation and apoptosis of erythroid progenitor cells".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 37.4(2007):291-299.
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