|PDGFs regulate tooth germ proliferation and ameloblast differentiation|
|Wu, Nan2,3,4; Iwamoto, Tsutomu1; Sugawara, Yu1; Futaki, Masaharu1; Yoshizaki, Keigo2; Yamamoto, Shinya2; Yamada, Aya1; Nakamura, Takashi1; Nonaka, Kazuaki2; Fukumoto, Satoshi1|
|关键词||Tooth development PDGF Organ culture Cusp Ameloblastin|
|刊名||ARCHIVES OF ORAL BIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||GROWTH-FACTORS ; MORPHOGENESIS ; ALPHA ; MICE ; EXPRESSION ; RECEPTOR ; MOUSE ; DEFICIENT ; ROLES ; CELLS|
Objective: The purpose of this study was to elucidate the effects of platelet-derived growth factors (PDGFs) during tooth development, as well as the mechanisms underlying the interactions cif growth factors with PDGF signalling during odontogenesis.
Design: We used an ex vivo tooth germ organ culture system and two dental cell lines, SF2 cells and mDP cells, as models of odontogenesis. AG17, a tyrosine kinase inhibitor, was utilised for blocking PDGF receptor signalling. To analyse the expressions of PDGFs, reverse transcriptase (RT)-PCR and immunohistochemistry were performed. Proliferation was examined using a BrdU incorporation assay for the organ cultures and a cell counting kit for the cell lines. The expressions of Fgf2 and ameloblastin were analysed by real-time RTPCR.
Results: The PDGF ligands PDGF-A and PDGF-B, and their receptors, PDGFRa and PDGFRB, were expressed throughout the initial stages of tooth development. In the tooth germ organ cultures, PDGF-AA, but not PDGF-BB, accelerated cusp formation. Conversely, AG17 suppressed both growth and cusp formation of tooth germs. Exogenous PDGF-BB promoted mDP cell proliferation. Furthermore, PDGF-AA decreased Fgf2 expression and increased that of ameloblastin, a marker of differentiated ameloblasts.
Conclusion: Our results indicate that PDGFs are involved in initial tooth development and regulate tooth size and shape, as well as ameloblast differentiation. (C) 2010 Elsevier Ltd. All rights reserved.
|项目编号||17689058 ; 20679006|
|资助机构||Ministry of Education, Science and Culture of Japan|
|作者单位||1.Tohoku Univ, Grad Sch Dent, Dept Oral Hlth & Dev Sci, Div Pediat Dent, Sendai, Miyagi 9808575, Japan|
2.Kyushu Univ, Fac Dent Sci, Div Oral Hlth Growth & Dev, Sect Pediat Dent, Fukuoka 8128582, Japan
3.Peking Univ, Dept Pediat Dent, Sch Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Dept Pediat Dent, Hosp Stomatol, Beijing 100081, Peoples R China
|Wu, Nan,Iwamoto, Tsutomu,Sugawara, Yu,et al. PDGFs regulate tooth germ proliferation and ameloblast differentiation[J]. ARCHIVES OF ORAL BIOLOGY,2010,55(6):426-434.|
|APA||Wu, Nan.,Iwamoto, Tsutomu.,Sugawara, Yu.,Futaki, Masaharu.,Yoshizaki, Keigo.,...&Fukumoto, Satoshi.(2010).PDGFs regulate tooth germ proliferation and ameloblast differentiation.ARCHIVES OF ORAL BIOLOGY,55(6),426-434.|
|MLA||Wu, Nan,et al."PDGFs regulate tooth germ proliferation and ameloblast differentiation".ARCHIVES OF ORAL BIOLOGY 55.6(2010):426-434.|