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学科主题: 口腔医学
题名:
PDGFs regulate tooth germ proliferation and ameloblast differentiation
作者: Wu, Nan2,3,4; Iwamoto, Tsutomu1; Sugawara, Yu1; Futaki, Masaharu1; Yoshizaki, Keigo2; Yamamoto, Shinya2; Yamada, Aya1; Nakamura, Takashi1; Nonaka, Kazuaki2; Fukumoto, Satoshi1
关键词: Tooth development ; PDGF ; Organ culture ; Cusp ; Ameloblastin
刊名: ARCHIVES OF ORAL BIOLOGY
发表日期: 2010-06-01
DOI: 10.1016/j.archoralbio.2010.03.011
卷: 55, 期:6, 页:426-434
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: GROWTH-FACTORS ; MORPHOGENESIS ; ALPHA ; MICE ; EXPRESSION ; RECEPTOR ; MOUSE ; DEFICIENT ; ROLES ; CELLS
英文摘要:

Objective: The purpose of this study was to elucidate the effects of platelet-derived growth factors (PDGFs) during tooth development, as well as the mechanisms underlying the interactions cif growth factors with PDGF signalling during odontogenesis.

Design: We used an ex vivo tooth germ organ culture system and two dental cell lines, SF2 cells and mDP cells, as models of odontogenesis. AG17, a tyrosine kinase inhibitor, was utilised for blocking PDGF receptor signalling. To analyse the expressions of PDGFs, reverse transcriptase (RT)-PCR and immunohistochemistry were performed. Proliferation was examined using a BrdU incorporation assay for the organ cultures and a cell counting kit for the cell lines. The expressions of Fgf2 and ameloblastin were analysed by real-time RTPCR.

Results: The PDGF ligands PDGF-A and PDGF-B, and their receptors, PDGFRa and PDGFRB, were expressed throughout the initial stages of tooth development. In the tooth germ organ cultures, PDGF-AA, but not PDGF-BB, accelerated cusp formation. Conversely, AG17 suppressed both growth and cusp formation of tooth germs. Exogenous PDGF-BB promoted mDP cell proliferation. Furthermore, PDGF-AA decreased Fgf2 expression and increased that of ameloblastin, a marker of differentiated ameloblasts.

Conclusion: Our results indicate that PDGFs are involved in initial tooth development and regulate tooth size and shape, as well as ameloblast differentiation. (C) 2010 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 17689058 ; 20679006
项目资助者: Ministry of Education, Science and Culture of Japan
WOS记录号: WOS:000279100100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62983
Appears in Collections:北京大学口腔医学院_儿童口腔科_期刊论文

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作者单位: 1.Tohoku Univ, Grad Sch Dent, Dept Oral Hlth & Dev Sci, Div Pediat Dent, Sendai, Miyagi 9808575, Japan
2.Kyushu Univ, Fac Dent Sci, Div Oral Hlth Growth & Dev, Sect Pediat Dent, Fukuoka 8128582, Japan
3.Peking Univ, Dept Pediat Dent, Sch Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Dept Pediat Dent, Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Wu, Nan,Iwamoto, Tsutomu,Sugawara, Yu,et al. PDGFs regulate tooth germ proliferation and ameloblast differentiation[J]. ARCHIVES OF ORAL BIOLOGY,2010,55(6):426-434.
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