IR@PKUHSC  > 北京大学口腔医学院  > 儿童口腔科
学科主题口腔医学
PDGFs regulate tooth germ proliferation and ameloblast differentiation
Wu, Nan2,3,4; Iwamoto, Tsutomu1; Sugawara, Yu1; Futaki, Masaharu1; Yoshizaki, Keigo2; Yamamoto, Shinya2; Yamada, Aya1; Nakamura, Takashi1; Nonaka, Kazuaki2; Fukumoto, Satoshi1
关键词Tooth development PDGF Organ culture Cusp Ameloblastin
刊名ARCHIVES OF ORAL BIOLOGY
2010-06-01
DOI10.1016/j.archoralbio.2010.03.011
55期:6页:426-434
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]GROWTH-FACTORS ; MORPHOGENESIS ; ALPHA ; MICE ; EXPRESSION ; RECEPTOR ; MOUSE ; DEFICIENT ; ROLES ; CELLS
英文摘要

Objective: The purpose of this study was to elucidate the effects of platelet-derived growth factors (PDGFs) during tooth development, as well as the mechanisms underlying the interactions cif growth factors with PDGF signalling during odontogenesis.

Design: We used an ex vivo tooth germ organ culture system and two dental cell lines, SF2 cells and mDP cells, as models of odontogenesis. AG17, a tyrosine kinase inhibitor, was utilised for blocking PDGF receptor signalling. To analyse the expressions of PDGFs, reverse transcriptase (RT)-PCR and immunohistochemistry were performed. Proliferation was examined using a BrdU incorporation assay for the organ cultures and a cell counting kit for the cell lines. The expressions of Fgf2 and ameloblastin were analysed by real-time RTPCR.

Results: The PDGF ligands PDGF-A and PDGF-B, and their receptors, PDGFRa and PDGFRB, were expressed throughout the initial stages of tooth development. In the tooth germ organ cultures, PDGF-AA, but not PDGF-BB, accelerated cusp formation. Conversely, AG17 suppressed both growth and cusp formation of tooth germs. Exogenous PDGF-BB promoted mDP cell proliferation. Furthermore, PDGF-AA decreased Fgf2 expression and increased that of ameloblastin, a marker of differentiated ameloblasts.

Conclusion: Our results indicate that PDGFs are involved in initial tooth development and regulate tooth size and shape, as well as ameloblast differentiation. (C) 2010 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000279100100005
项目编号17689058 ; 20679006
资助机构Ministry of Education, Science and Culture of Japan
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62983
专题北京大学口腔医学院_儿童口腔科
作者单位1.Tohoku Univ, Grad Sch Dent, Dept Oral Hlth & Dev Sci, Div Pediat Dent, Sendai, Miyagi 9808575, Japan
2.Kyushu Univ, Fac Dent Sci, Div Oral Hlth Growth & Dev, Sect Pediat Dent, Fukuoka 8128582, Japan
3.Peking Univ, Dept Pediat Dent, Sch Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Dept Pediat Dent, Hosp Stomatol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Wu, Nan,Iwamoto, Tsutomu,Sugawara, Yu,et al. PDGFs regulate tooth germ proliferation and ameloblast differentiation[J]. ARCHIVES OF ORAL BIOLOGY,2010,55(6):426-434.
APA Wu, Nan.,Iwamoto, Tsutomu.,Sugawara, Yu.,Futaki, Masaharu.,Yoshizaki, Keigo.,...&Fukumoto, Satoshi.(2010).PDGFs regulate tooth germ proliferation and ameloblast differentiation.ARCHIVES OF ORAL BIOLOGY,55(6),426-434.
MLA Wu, Nan,et al."PDGFs regulate tooth germ proliferation and ameloblast differentiation".ARCHIVES OF ORAL BIOLOGY 55.6(2010):426-434.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wu, Nan]的文章
[Iwamoto, Tsutomu]的文章
[Sugawara, Yu]的文章
百度学术
百度学术中相似的文章
[Wu, Nan]的文章
[Iwamoto, Tsutomu]的文章
[Sugawara, Yu]的文章
必应学术
必应学术中相似的文章
[Wu, Nan]的文章
[Iwamoto, Tsutomu]的文章
[Sugawara, Yu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。