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Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase
Zheng, Shuai1; Li, Wenjing1; Xu, Mingjiang1; Bai, Xue1; Zhou, Zhou1; Han, Jingyan2; Shyy, John Y-J3; Wang, Xian1
关键词vasoactive peptide endothelial cell endothelial nitric oxide synthase
刊名AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
2010-12-01
DOI10.1152/ajpcell.00173.2010
299期:6页:C1485-C1492
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
资助者Major National Basic Research Program of China ; 111 Project ; Major National Basic Research Program of China ; 111 Project
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]VEIN ENDOTHELIAL-CELLS ; NITRIC-OXIDE ; IN-VITRO ; SKELETAL-MUSCLE ; TISSUE ISCHEMIA ; RAT ; EXPRESSION ; ADRENOMEDULLIN ; FACILITATION ; RECEPTORS
英文摘要

Zheng S, Li W, Xu M, Bai X, Zhou Z, Han J, Shyy JY, Wang X. Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase. Am J Physiol Cell Physiol 299: C1485-C1492, 2010. First published September 29, 2010; doi:10.1152/ajpcell.00173.2010.-Ischemia induces angiogenesis as a compensatory response. Although ischemia is known to causes synthesis and release of calcitonin gene-related peptide (CGRP), it is not clear whether CGRP regulates angiogenesis under ischemia and how does it function. Thus we investigated the role of CGRP in angiogenesis and the involved mechanisms. We found that CGRP level was increased in the rat hindlimb ischemic tissue. The expression of exogenous CGRP by adenovirus vectors enhanced blood flow recovery and increased capillary density in ischemic hindlimbs. In vitro, CGRP promoted human umbilical vein endothelial cell (HUVEC) tube formation and migration. Further more, CGRP activated AMP-activated protein kinase (AMPK) both in vivo and in vitro, and pharmacological inhibition of CGRP and cAMP attenuated the CGRP-activated AMPK in vitro. CGRP also induced endothelial nitric oxide synthase (eNOS) phosphorylation in HUVECs at Ser1177 and Ser633 in a time-dependent manner, and such effects were abolished by AMPK inhibitor Compound C. As well, Compound C blocked CGRP-enhanced HUVEC tube formation and migration. These findings indicate that CGRP promotes angiogenesis by activating the AMPK-eNOS pathway in endothelial cells.

语种英语
所属项目编号2006CB503802 ; B07001
资助者Major National Basic Research Program of China ; 111 Project ; Major National Basic Research Program of China ; 111 Project
WOS记录号WOS:000284822100030
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62986
专题基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol,Minist Educ, Hlth Sci Ctr,Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Microcirculat Res Ctr,Minist Educ, Hlth Sci Ctr,Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA
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GB/T 7714
Zheng, Shuai,Li, Wenjing,Xu, Mingjiang,et al. Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2010,299(6):C1485-C1492.
APA Zheng, Shuai.,Li, Wenjing.,Xu, Mingjiang.,Bai, Xue.,Zhou, Zhou.,...&Wang, Xian.(2010).Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,299(6),C1485-C1492.
MLA Zheng, Shuai,et al."Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 299.6(2010):C1485-C1492.
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