IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase
Zheng, Shuai1; Li, Wenjing1; Xu, Mingjiang1; Bai, Xue1; Zhou, Zhou1; Han, Jingyan2; Shyy, John Y-J3; Wang, Xian1
关键词vasoactive peptide endothelial cell endothelial nitric oxide synthase
刊名AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
2010-12-01
DOI10.1152/ajpcell.00173.2010
299期:6页:C1485-C1492
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]VEIN ENDOTHELIAL-CELLS ; NITRIC-OXIDE ; IN-VITRO ; SKELETAL-MUSCLE ; TISSUE ISCHEMIA ; RAT ; EXPRESSION ; ADRENOMEDULLIN ; FACILITATION ; RECEPTORS
英文摘要

Zheng S, Li W, Xu M, Bai X, Zhou Z, Han J, Shyy JY, Wang X. Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase. Am J Physiol Cell Physiol 299: C1485-C1492, 2010. First published September 29, 2010; doi:10.1152/ajpcell.00173.2010.-Ischemia induces angiogenesis as a compensatory response. Although ischemia is known to causes synthesis and release of calcitonin gene-related peptide (CGRP), it is not clear whether CGRP regulates angiogenesis under ischemia and how does it function. Thus we investigated the role of CGRP in angiogenesis and the involved mechanisms. We found that CGRP level was increased in the rat hindlimb ischemic tissue. The expression of exogenous CGRP by adenovirus vectors enhanced blood flow recovery and increased capillary density in ischemic hindlimbs. In vitro, CGRP promoted human umbilical vein endothelial cell (HUVEC) tube formation and migration. Further more, CGRP activated AMP-activated protein kinase (AMPK) both in vivo and in vitro, and pharmacological inhibition of CGRP and cAMP attenuated the CGRP-activated AMPK in vitro. CGRP also induced endothelial nitric oxide synthase (eNOS) phosphorylation in HUVECs at Ser1177 and Ser633 in a time-dependent manner, and such effects were abolished by AMPK inhibitor Compound C. As well, Compound C blocked CGRP-enhanced HUVEC tube formation and migration. These findings indicate that CGRP promotes angiogenesis by activating the AMPK-eNOS pathway in endothelial cells.

语种英语
WOS记录号WOS:000284822100030
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62986
Collection北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol,Minist Educ, Hlth Sci Ctr,Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Microcirculat Res Ctr,Minist Educ, Hlth Sci Ctr,Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA
Recommended Citation
GB/T 7714
Zheng, Shuai,Li, Wenjing,Xu, Mingjiang,et al. Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2010,299(6):C1485-C1492.
APA Zheng, Shuai.,Li, Wenjing.,Xu, Mingjiang.,Bai, Xue.,Zhou, Zhou.,...&Wang, Xian.(2010).Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,299(6),C1485-C1492.
MLA Zheng, Shuai,et al."Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 299.6(2010):C1485-C1492.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Zheng, Shuai]'s Articles
[Li, Wenjing]'s Articles
[Xu, Mingjiang]'s Articles
百度学术
百度学术Similar articles in
[Zheng, Shuai]'s Articles
[Li, Wenjing]'s Articles
[Xu, Mingjiang]'s Articles
必应学术
必应学术Similar articles in
[Zheng, Shuai]'s Articles
[Li, Wenjing]'s Articles
[Xu, Mingjiang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.