北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase
作者: Zheng, Shuai1; Li, Wenjing1; Xu, Mingjiang1; Bai, Xue1; Zhou, Zhou1; Han, Jingyan2; Shyy, John Y-J3; Wang, Xian1
关键词: vasoactive peptide ; endothelial cell ; endothelial nitric oxide synthase
刊名: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
发表日期: 2010-12-01
DOI: 10.1152/ajpcell.00173.2010
卷: 299, 期:6, 页:C1485-C1492
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Physiology
研究领域[WOS]: Cell Biology ; Physiology
关键词[WOS]: VEIN ENDOTHELIAL-CELLS ; NITRIC-OXIDE ; IN-VITRO ; SKELETAL-MUSCLE ; TISSUE ISCHEMIA ; RAT ; EXPRESSION ; ADRENOMEDULLIN ; FACILITATION ; RECEPTORS
英文摘要:

Zheng S, Li W, Xu M, Bai X, Zhou Z, Han J, Shyy JY, Wang X. Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase. Am J Physiol Cell Physiol 299: C1485-C1492, 2010. First published September 29, 2010; doi:10.1152/ajpcell.00173.2010.-Ischemia induces angiogenesis as a compensatory response. Although ischemia is known to causes synthesis and release of calcitonin gene-related peptide (CGRP), it is not clear whether CGRP regulates angiogenesis under ischemia and how does it function. Thus we investigated the role of CGRP in angiogenesis and the involved mechanisms. We found that CGRP level was increased in the rat hindlimb ischemic tissue. The expression of exogenous CGRP by adenovirus vectors enhanced blood flow recovery and increased capillary density in ischemic hindlimbs. In vitro, CGRP promoted human umbilical vein endothelial cell (HUVEC) tube formation and migration. Further more, CGRP activated AMP-activated protein kinase (AMPK) both in vivo and in vitro, and pharmacological inhibition of CGRP and cAMP attenuated the CGRP-activated AMPK in vitro. CGRP also induced endothelial nitric oxide synthase (eNOS) phosphorylation in HUVECs at Ser1177 and Ser633 in a time-dependent manner, and such effects were abolished by AMPK inhibitor Compound C. As well, Compound C blocked CGRP-enhanced HUVEC tube formation and migration. These findings indicate that CGRP promotes angiogenesis by activating the AMPK-eNOS pathway in endothelial cells.

语种: 英语
所属项目编号: 2006CB503802 ; B07001
项目资助者: Major National Basic Research Program of China ; 111 Project
WOS记录号: WOS:000284822100030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62986
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol,Minist Educ, Hlth Sci Ctr,Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Microcirculat Res Ctr,Minist Educ, Hlth Sci Ctr,Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA

Recommended Citation:
Zheng, Shuai,Li, Wenjing,Xu, Mingjiang,et al. Calcitonin gene-related peptide promotes angiogenesis via AMP-activated protein kinase[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2010,299(6):C1485-C1492.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zheng, Shuai]'s Articles
[Li, Wenjing]'s Articles
[Xu, Mingjiang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zheng, Shuai]‘s Articles
[Li, Wenjing]‘s Articles
[Xu, Mingjiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace