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学科主题: 临床医学
题名:
Radioactive iodine labeling of monoclonal antibody against Hsp90 alpha and its use in diagnostic imaging in prostate cancer xenograft model
作者: Sun, H. -W.; Wang, R. -F.; Yan, P.; Zhang, C. -L.; Hao, P.; Ma, H.; Chen, X. -Q.
关键词: Hsp90 alpha ; Monoclonal antibody ; Molecular imaging ; I-131 ; Radiolabel
刊名: EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
发表日期: 2015-03-01
卷: 19, 期:5, 页:835-843
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: ELEVATED SERUM THYROGLOBULIN ; THYROID-CANCER ; HEAT-SHOCK ; HSP90 ; DEGRADATION ; PROTEINS ; THERAPY ; CELLS ; I-131
英文摘要:

OBJECTIVE: Heat shock protein (Hsp90) resides exclusively in the cytosol in normal cells, but is activated and then removes to the cell surface in tumor cells. The detecting up-regulation or activation of Hsp90 is an early indicator of malignant behavior of cancer cells. Hsp90 has emerged as an important target for diagnosis or therapy of prostate cancer. In this study, we labeled Hsp90 alpha specific monoclonal antibody (Hsp90 alpha-mAb) with radioiodine (NaI)-I-131 and investigated its potential usage in diagnostic imaging of prostate tumor in xenograft mice model.

METHODS: Hsp90 alpha-mAb was radioiodinated by using chloramine-T. The radiolabeling efficiency and radiochemical purity were assessed in vitro. I-131-Hsp90 alpha-mAb was then injected into the nude mice bearing human prostate carcinoma. The planar gamma Imaging was performed at 3, 6, 9 and 12 h after injection.

RESULTS: The radiochemical purity of I-131-Hsp90 alpha-mAb exceeded 95% after purification. This radiolabeled mAb was stable in human blood serum. In planar gamma imaging study, the prostate tumors in mice model were imaged clearly at 3h after injection of I-131-Hsp90 alpha-mAb.

CONCLUSIONS: The results suggest that I-131-HSP90 alpha-mAb could be a new promising molecular probe for diagnostic imaging of prostate tumors.

语种: 英语
所属项目编号: NSFC 81071183 ; 2011YQ03011409 ; 2014BAA03B03
项目资助者: National Natural Science Foundation of China ; National Major Scientific Equipment Special Fund ; Key Projects in the National Science &amp ; Technology Pillar Program during the Twelve Five-year Plan Period
WOS记录号: WOS:000352642700021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62995
Appears in Collections:北京大学第一临床医学院_核医学科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Nucl Med, Beijing 100871, Peoples R China

Recommended Citation:
Sun, H. -W.,Wang, R. -F.,Yan, P.,et al. Radioactive iodine labeling of monoclonal antibody against Hsp90 alpha and its use in diagnostic imaging in prostate cancer xenograft model[J]. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2015,19(5):835-843.
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