IR@PKUHSC  > 北京大学第一临床医学院  > 核医学科
学科主题临床医学
Radioactive iodine labeling of monoclonal antibody against Hsp90 alpha and its use in diagnostic imaging in prostate cancer xenograft model
Sun, H. -W.; Wang, R. -F.; Yan, P.; Zhang, C. -L.; Hao, P.; Ma, H.; Chen, X. -Q.
关键词Hsp90 alpha Monoclonal antibody Molecular imaging I-131 Radiolabel
刊名EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
2015-03-01
19期:5页:835-843
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]ELEVATED SERUM THYROGLOBULIN ; THYROID-CANCER ; HEAT-SHOCK ; HSP90 ; DEGRADATION ; PROTEINS ; THERAPY ; CELLS ; I-131
英文摘要

OBJECTIVE: Heat shock protein (Hsp90) resides exclusively in the cytosol in normal cells, but is activated and then removes to the cell surface in tumor cells. The detecting up-regulation or activation of Hsp90 is an early indicator of malignant behavior of cancer cells. Hsp90 has emerged as an important target for diagnosis or therapy of prostate cancer. In this study, we labeled Hsp90 alpha specific monoclonal antibody (Hsp90 alpha-mAb) with radioiodine (NaI)-I-131 and investigated its potential usage in diagnostic imaging of prostate tumor in xenograft mice model.

METHODS: Hsp90 alpha-mAb was radioiodinated by using chloramine-T. The radiolabeling efficiency and radiochemical purity were assessed in vitro. I-131-Hsp90 alpha-mAb was then injected into the nude mice bearing human prostate carcinoma. The planar gamma Imaging was performed at 3, 6, 9 and 12 h after injection.

RESULTS: The radiochemical purity of I-131-Hsp90 alpha-mAb exceeded 95% after purification. This radiolabeled mAb was stable in human blood serum. In planar gamma imaging study, the prostate tumors in mice model were imaged clearly at 3h after injection of I-131-Hsp90 alpha-mAb.

CONCLUSIONS: The results suggest that I-131-HSP90 alpha-mAb could be a new promising molecular probe for diagnostic imaging of prostate tumors.

语种英语
WOS记录号WOS:000352642700021
项目编号NSFC 81071183 ; 2011YQ03011409 ; 2014BAA03B03
资助机构National Natural Science Foundation of China ; National Major Scientific Equipment Special Fund ; Key Projects in the National Science &amp ; Technology Pillar Program during the Twelve Five-year Plan Period
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62995
专题北京大学第一临床医学院_核医学科
作者单位Peking Univ, Hosp 1, Dept Nucl Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Sun, H. -W.,Wang, R. -F.,Yan, P.,et al. Radioactive iodine labeling of monoclonal antibody against Hsp90 alpha and its use in diagnostic imaging in prostate cancer xenograft model[J]. EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2015,19(5):835-843.
APA Sun, H. -W..,Wang, R. -F..,Yan, P..,Zhang, C. -L..,Hao, P..,...&Chen, X. -Q..(2015).Radioactive iodine labeling of monoclonal antibody against Hsp90 alpha and its use in diagnostic imaging in prostate cancer xenograft model.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,19(5),835-843.
MLA Sun, H. -W.,et al."Radioactive iodine labeling of monoclonal antibody against Hsp90 alpha and its use in diagnostic imaging in prostate cancer xenograft model".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 19.5(2015):835-843.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, H. -W.]的文章
[Wang, R. -F.]的文章
[Yan, P.]的文章
百度学术
百度学术中相似的文章
[Sun, H. -W.]的文章
[Wang, R. -F.]的文章
[Yan, P.]的文章
必应学术
必应学术中相似的文章
[Sun, H. -W.]的文章
[Wang, R. -F.]的文章
[Yan, P.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。