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Durability of direct immunofluorescence (DIF) slides stored at room temperature
Elbendary, Amira1,2; Zhou, Cheng1,3; Truong, Jonathan1; Elston, Dirk M.1
关键词direct immunofluorescence room temperature storing
刊名JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
2015-12-01
DOI10.1016/j.jaad.2015.07.027
73期:6页:1021-1024
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology
研究领域[WOS]Dermatology
关键词[WOS]CUTANEOUS IMMUNOFLUORESCENCE ; MICROSCOPY ; STORAGE
英文摘要

Background: Prior studies suggested that direct immunofluorescence (DIF) slides can be stored at room temperature.

Objectives: We sought to determine the durability of DIF slides stored at room temperature for 5 years.

Methods: This was a retrospective study of 83 DIF slides archived at room temperature during 2010. The pattern of immunoreactants was compared with those noted in the original report.

Results: Loss of reactivity was limited to cases with weak fluorescence at original diagnosis. Loss of IgG was noted in 12.5% of cases, IgA in 12%, C3 in 10%, and IgM in 9.75%. Fibrin showed no loss of reactivity. Preservation of immunofluorescence was not related to site of deposition. Overall, a reliable diagnosis could be made in 75 of 79 archived cases (94.9%).

Limitations: Cases had been archived for periods varying from 4.5 to 5 years. Variations in processing and fluorochromes could affect durability. We have no way of knowing how long slides had been exposed to ultraviolet light at the time of initial examination.

Conclusion: DIF showed excellent durability in slides kept at room temperature for 5 years.

语种英语
WOS记录号WOS:000365109300025
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63007
专题北京大学第二临床医学院_皮科
作者单位1.Ackerman Acad Dermatopathol, New York, NY USA
2.Cairo Univ, Dept Dermatol, Kasr Al Ainy Fac Med, Cairo, Egypt
3.Peking Univ, Dept Dermatol, Peoples Hosp, Beijing 100871, Peoples R China
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GB/T 7714
Elbendary, Amira,Zhou, Cheng,Truong, Jonathan,et al. Durability of direct immunofluorescence (DIF) slides stored at room temperature[J]. JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY,2015,73(6):1021-1024.
APA Elbendary, Amira,Zhou, Cheng,Truong, Jonathan,&Elston, Dirk M..(2015).Durability of direct immunofluorescence (DIF) slides stored at room temperature.JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY,73(6),1021-1024.
MLA Elbendary, Amira,et al."Durability of direct immunofluorescence (DIF) slides stored at room temperature".JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY 73.6(2015):1021-1024.
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