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学科主题: 临床医学
题名:
Durability of direct immunofluorescence (DIF) slides stored at room temperature
作者: Elbendary, Amira1,2; Zhou, Cheng1,3; Truong, Jonathan1; Elston, Dirk M.1
关键词: direct immunofluorescence ; room temperature ; storing
刊名: JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
发表日期: 2015-12-01
DOI: 10.1016/j.jaad.2015.07.027
卷: 73, 期:6, 页:1021-1024
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology
研究领域[WOS]: Dermatology
关键词[WOS]: CUTANEOUS IMMUNOFLUORESCENCE ; MICROSCOPY ; STORAGE
英文摘要:

Background: Prior studies suggested that direct immunofluorescence (DIF) slides can be stored at room temperature.

Objectives: We sought to determine the durability of DIF slides stored at room temperature for 5 years.

Methods: This was a retrospective study of 83 DIF slides archived at room temperature during 2010. The pattern of immunoreactants was compared with those noted in the original report.

Results: Loss of reactivity was limited to cases with weak fluorescence at original diagnosis. Loss of IgG was noted in 12.5% of cases, IgA in 12%, C3 in 10%, and IgM in 9.75%. Fibrin showed no loss of reactivity. Preservation of immunofluorescence was not related to site of deposition. Overall, a reliable diagnosis could be made in 75 of 79 archived cases (94.9%).

Limitations: Cases had been archived for periods varying from 4.5 to 5 years. Variations in processing and fluorochromes could affect durability. We have no way of knowing how long slides had been exposed to ultraviolet light at the time of initial examination.

Conclusion: DIF showed excellent durability in slides kept at room temperature for 5 years.

语种: 英语
所属项目编号: 201406015016
项目资助者: China Scholarship Council
WOS记录号: WOS:000365109300025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63007
Appears in Collections:北京大学第二临床医学院_皮科_期刊论文

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作者单位: 1.Ackerman Acad Dermatopathol, New York, NY USA
2.Cairo Univ, Dept Dermatol, Kasr Al Ainy Fac Med, Cairo, Egypt
3.Peking Univ, Dept Dermatol, Peoples Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Elbendary, Amira,Zhou, Cheng,Truong, Jonathan,et al. Durability of direct immunofluorescence (DIF) slides stored at room temperature[J]. JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY,2015,73(6):1021-1024.
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