|Durability of direct immunofluorescence (DIF) slides stored at room temperature|
|Elbendary, Amira1,2; Zhou, Cheng1,3; Truong, Jonathan1; Elston, Dirk M.1|
|关键词||direct immunofluorescence room temperature storing|
|刊名||JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||CUTANEOUS IMMUNOFLUORESCENCE ; MICROSCOPY ; STORAGE|
Background: Prior studies suggested that direct immunofluorescence (DIF) slides can be stored at room temperature.
Objectives: We sought to determine the durability of DIF slides stored at room temperature for 5 years.
Methods: This was a retrospective study of 83 DIF slides archived at room temperature during 2010. The pattern of immunoreactants was compared with those noted in the original report.
Results: Loss of reactivity was limited to cases with weak fluorescence at original diagnosis. Loss of IgG was noted in 12.5% of cases, IgA in 12%, C3 in 10%, and IgM in 9.75%. Fibrin showed no loss of reactivity. Preservation of immunofluorescence was not related to site of deposition. Overall, a reliable diagnosis could be made in 75 of 79 archived cases (94.9%).
Limitations: Cases had been archived for periods varying from 4.5 to 5 years. Variations in processing and fluorochromes could affect durability. We have no way of knowing how long slides had been exposed to ultraviolet light at the time of initial examination.
Conclusion: DIF showed excellent durability in slides kept at room temperature for 5 years.
|资助机构||China Scholarship Council|
|作者单位||1.Ackerman Acad Dermatopathol, New York, NY USA|
2.Cairo Univ, Dept Dermatol, Kasr Al Ainy Fac Med, Cairo, Egypt
3.Peking Univ, Dept Dermatol, Peoples Hosp, Beijing 100871, Peoples R China
|Elbendary, Amira,Zhou, Cheng,Truong, Jonathan,et al. Durability of direct immunofluorescence (DIF) slides stored at room temperature[J]. JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY,2015,73(6):1021-1024.|
|APA||Elbendary, Amira,Zhou, Cheng,Truong, Jonathan,&Elston, Dirk M..(2015).Durability of direct immunofluorescence (DIF) slides stored at room temperature.JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY,73(6),1021-1024.|
|MLA||Elbendary, Amira,et al."Durability of direct immunofluorescence (DIF) slides stored at room temperature".JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY 73.6(2015):1021-1024.|