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学科主题: 临床医学
题名:
Effect of growth hormone receptor gene disruption and PMA treatment on the expression of genes involved in primordial follicle activation in mice ovaries
作者: Schneider, Augusto1; Zhi, Xu2,3; Bartke, Andrzej4; Kopchick, John J.5; Masternak, Michal M.3,6
关键词: GHRKO ; FOXO3a ; AMH ; Aging ; Ovary
刊名: AGE
发表日期: 2014-08-01
DOI: 10.1007/s11357-014-9701-9
卷: 36, 期:4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Geriatrics & Gerontology
研究领域[WOS]: Geriatrics & Gerontology
关键词[WOS]: FEMALE REPRODUCTIVE FUNCTION ; DWARF MICE ; IGF-I ; DIFFERENTIATION FACTOR-9 ; CALORIC RESTRICTION ; MESSENGER-RNA ; KNOCKOUT MICE ; MOUSE ; RESISTANCE ; OOCYTES
英文摘要:

The activation of the Pi3k-Akt1-FOXO pathway seems to be involved in the extended longevity observed in growth hormone receptor/growth hormone binding protein knockout (GHRKO) mice and is related to the growth of primordial ovarian follicles. The aim of this work was to measure the expression of genes in the ovaries of GHRKO and normal (N) mice treated with phorbol 12-myristate 13-acetate (PMA), an inhibitor of GH and IRS1 signaling. For this study, a group of N (n=10) and GHRKO (n=10) mice, N mice treated (n=10) or not (n=10) with PMA, and GHRKOmice treated (n=10) or not (n=10) with PMA were used. All were 6-month-old female mice. After the last PMA injection, the ovaries were collected for gene expression analysis. Expression of Amh, Gdf9, and Bmp15 was higher in GHRKO than N mice (P<0.05), but was not different between PMA-treated N mice (P>0.10). Expression of Amh and Gdf9 was higher (P<0.05) for GHRKO PMA-treated mice. In addition, we observed a higher expression of Socs3 (P<0.001) in GHRKO than N mice and a tendency for increased expression of Foxo3a (P=0.07). For GHRKO PMA-treated mice, Foxo3a mRNA expression was higher (P=0.02) and a tendency for higher expression of Mtor (P=0.06) and Socs3 (P=0.10) in GHRKO PMA-treated mice was observed. To summarize, the present data further confirm the previous histological observations that GHRKO mice have an ovarian phenotype characteristic of younger mice indicated by higher expression of Amh, Gdf9, and Bmp15 mRNA. In addition, we have shown a higher expression of Socs3 in GHRKO mice and higher Foxo3a expression in PMA-treated GHRKO mice, suggesting a role for these mediators in the process of ovarian aging.

语种: 英语
所属项目编号: R01AG032290 ; P01AG031736
项目资助者: National Institute on Aging of the National Institutes of Health ; Federal University of Pelotas
WOS记录号: WOS:000343801100035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63009
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Univ Fed Pelotas, Dept Nutr, Pelotas, RS, Brazil
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Univ Cent Florida, Burnett Sch Biomed Sci, Coll Med, Orlando, FL 32827 USA
4.So Illinois Univ, Sch Med, Dept Internal Med, Springfield, IL 62794 USA
5.Ohio Univ, Dept Biomed Sci, Edison Biotechnol Inst, Athens, OH 45701 USA
6.Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland

Recommended Citation:
Schneider, Augusto,Zhi, Xu,Bartke, Andrzej,et al. Effect of growth hormone receptor gene disruption and PMA treatment on the expression of genes involved in primordial follicle activation in mice ovaries[J]. AGE,2014,36(4).
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