|MEPE is downregulated as dental pulp stem cells differentiate|
|Liu, H; Li, W; Shi, ST; Habelitz, S; Gao, C; DenBesten, P|
|关键词||MEPE DSP proliferation differentiation dental pulp stem cells DPSC osteogenesis superarray TGF-beta|
|刊名||ARCHIVES OF ORAL BIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||ODONTOBLAST-LIKE CELLS ; IN-VITRO ; ALKALINE-PHOSPHATASE ; HUMAN TEETH ; BONE SIALOPROTEIN ; MATRIX PROTEIN-1 ; MESSENGER-RNA ; GROWTH-FACTOR ; EXPRESSION ; RAT|
Previous studies on dental pulp cell culture have described heterogenous mixtures of cells that differentiate into odontoblasts and form mineralized dentin.
Objective: The aim of this study was to characterize the matrix extracellular phosphogtycoprotein (MEPE) expression by dental pulp stem cells (DPSC), related to cell differentiation.
Design: DPSC differentiation to form mineralized nodules was characterized by Alizarin red staining and micro-Raman spectroscopy. Osteogenesis SuperArray analysis was used to broadly screen for osteogenesis- related genes altered by DPSC differentiation. Relative levels of expression of MEPE and DSP were determined by serniquantitative RT-PCR and Western blot.
Results: Mineral analysis showed that as DPSC differentiated, they formed a carbonated hydroxyapatite mineral. Differentiation was initially marked by upregulation by Runx2, TGF beta-related genes, EGFR and genes involved in collagen metabolism. ALP activity first increased, as DPSCs reached confluence but later decreased when cells further differentiated three weeks after confluence. MEPE was the only marker that was downregutated as DPSCs differentiated.
Conclusion: DPSC differentiation can be characterized by downregulation of MEPE as other markers of DPSC differentiation, such as DSP, are upregulated. Expression of MEPE related to DSP and can be used to monitor DPSC as they are used for studies of odontobtast differentiation, tissue engineering or vital pulp therapy. The downregulation of MEPE as DPSC differentiate, suggests that MEPE is an inhibitor of mineralization. (c) 2005 Elsevier Ltd. All rights reserved.
|作者单位||1.Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China|
2.Univ Calif San Francisco, San Francisco, CA 94143 USA
3.NIDCR, Bethesda, MD USA
|Liu, H,Li, W,Shi, ST,et al. MEPE is downregulated as dental pulp stem cells differentiate[J]. ARCHIVES OF ORAL BIOLOGY,2005,50(11):923-928.|
|APA||Liu, H,Li, W,Shi, ST,Habelitz, S,Gao, C,&DenBesten, P.(2005).MEPE is downregulated as dental pulp stem cells differentiate.ARCHIVES OF ORAL BIOLOGY,50(11),923-928.|
|MLA||Liu, H,et al."MEPE is downregulated as dental pulp stem cells differentiate".ARCHIVES OF ORAL BIOLOGY 50.11(2005):923-928.|