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学科主题口腔医学
MEPE is downregulated as dental pulp stem cells differentiate
Liu, H; Li, W; Shi, ST; Habelitz, S; Gao, C; DenBesten, P
关键词MEPE DSP proliferation differentiation dental pulp stem cells DPSC osteogenesis superarray TGF-beta
刊名ARCHIVES OF ORAL BIOLOGY
2005-11-01
DOI10.1016/j.albchoralbio.2005.03.003
50期:11页:923-928
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]ODONTOBLAST-LIKE CELLS ; IN-VITRO ; ALKALINE-PHOSPHATASE ; HUMAN TEETH ; BONE SIALOPROTEIN ; MATRIX PROTEIN-1 ; MESSENGER-RNA ; GROWTH-FACTOR ; EXPRESSION ; RAT
英文摘要

Previous studies on dental pulp cell culture have described heterogenous mixtures of cells that differentiate into odontoblasts and form mineralized dentin.

Objective: The aim of this study was to characterize the matrix extracellular phosphogtycoprotein (MEPE) expression by dental pulp stem cells (DPSC), related to cell differentiation.

Design: DPSC differentiation to form mineralized nodules was characterized by Alizarin red staining and micro-Raman spectroscopy. Osteogenesis SuperArray analysis was used to broadly screen for osteogenesis- related genes altered by DPSC differentiation. Relative levels of expression of MEPE and DSP were determined by serniquantitative RT-PCR and Western blot.

Results: Mineral analysis showed that as DPSC differentiated, they formed a carbonated hydroxyapatite mineral. Differentiation was initially marked by upregulation by Runx2, TGF beta-related genes, EGFR and genes involved in collagen metabolism. ALP activity first increased, as DPSCs reached confluence but later decreased when cells further differentiated three weeks after confluence. MEPE was the only marker that was downregutated as DPSCs differentiated.

Conclusion: DPSC differentiation can be characterized by downregulation of MEPE as other markers of DPSC differentiation, such as DSP, are upregulated. Expression of MEPE related to DSP and can be used to monitor DPSC as they are used for studies of odontobtast differentiation, tissue engineering or vital pulp therapy. The downregulation of MEPE as DPSC differentiate, suggests that MEPE is an inhibitor of mineralization. (c) 2005 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000233312900001
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被引频次:54[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63015
专题北京大学口腔医学院
作者单位1.Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China
2.Univ Calif San Francisco, San Francisco, CA 94143 USA
3.NIDCR, Bethesda, MD USA
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GB/T 7714
Liu, H,Li, W,Shi, ST,et al. MEPE is downregulated as dental pulp stem cells differentiate[J]. ARCHIVES OF ORAL BIOLOGY,2005,50(11):923-928.
APA Liu, H,Li, W,Shi, ST,Habelitz, S,Gao, C,&DenBesten, P.(2005).MEPE is downregulated as dental pulp stem cells differentiate.ARCHIVES OF ORAL BIOLOGY,50(11),923-928.
MLA Liu, H,et al."MEPE is downregulated as dental pulp stem cells differentiate".ARCHIVES OF ORAL BIOLOGY 50.11(2005):923-928.
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