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学科主题: 口腔医学
题名:
Bacterial Infection Increases Periodontal Bone Loss in Diabetic Rats through Enhanced Apoptosis
作者: Pacios, Sandra1; Andriankaja, Oelisoa1,2; Kang, Jun3; Alnammary, Maher1; Bae, Jason1; Bezerra, Beatriz de Brito4; Schreiner, Helen5; Fine, Daniel H.5; Graves, Dana T.1
刊名: AMERICAN JOURNAL OF PATHOLOGY
发表日期: 2013-12-01
DOI: 10.1016/j.ajpath.2013.08.017
卷: 183, 期:6, 页:1928-1935
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: CASPASE-3 ACTIVATION ; OXIDATIVE STRESS ; IN-VIVO ; INFLAMMATION ; GENES ; ACTINOMYCETEMCOMITANS ; FIBROBLASTS ; OSTEOBLASTS ; OSTEOCLASTS ; RESORPTION
英文摘要:

Periodontal disease is the most common osteolytic disease in humans and is significantly increased by diabetes mellitus. We tested the hypothesis that bacterial infection induces bone loss in diabetic animals through a mechanism that involves enhanced apoptosis. Type II diabetic rats were inoculated with Aggregatibacter actinomycetemcomitans and treated with a caspase-3 inhibitor, ZDEVD-FMK, or vehicle alone. Apoptotic cells were measured with TUNEL; osteoblasts and bone area were measured in H&E sections. New bone formation was assessed by labeling with fluorescent dyes and by osteocalcin mRNA levels. Osteoclast number, eroded bone surface, and new bone formation were measured by tartrate-resistant acid phosphatase staining. Immunohistochemistry was performed with an antibody against tumor necrosis factor-alpha. Bacterial infection doubled the number of tumor necrosis factor-alpha-expressing cells and increased apoptotic cells adjacent to bone 10-fold (P < 0.05). Treatment with caspase inhibitor blocked apoptosis, increased the number of osteoclasts, and eroded bone surface (P < 0.05); yet, inhibition of apoptosis resulted in significantly greater net bone area because of an increase in new bone formation, osteoblast numbers, and an increase in bone coupling. Thus, bacterial infection in diabetic rats stimulates periodontitis, in part through enhanced apoptosis of osteoblastic cells that reduces osseous coupling through a caspase-3 dependent mechanism.

语种: 英语
所属项目编号: DE017732 ; P30AR050950
项目资助者: NIH ; Penn Center for Musculoskeletal Disorders from NIAMS ; Coordination for the Improvement of Higher Education Personnel (CAPES) Foundation in Brazil
WOS记录号: WOS:000327829000023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63016
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Univ Penn, Dept Periodont, Sch Dent Med, Philadelphia, PA 19104 USA
2.Univ Puerto Rico, Sch Dent Med, Ctr Clin Res & Hlth Promot, San Juan, PR 00936 USA
3.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100871, Peoples R China
4.Univ Estadual Campinas, Piracicaba Dent Sch, Prosthodont & Periodont Dept, Piracicaba, Brazil
5.Univ Med & Dent New Jersey, New Jersey Dent Sch, Dept Oral Biol, Newark, NJ 07103 USA

Recommended Citation:
Pacios, Sandra,Andriankaja, Oelisoa,Kang, Jun,et al. Bacterial Infection Increases Periodontal Bone Loss in Diabetic Rats through Enhanced Apoptosis[J]. AMERICAN JOURNAL OF PATHOLOGY,2013,183(6):1928-1935.
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