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学科主题药学
Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes
Zhao, Zhihui1,4; Li, Qing2; Hu, Jiale1,3; Li, Zhongjun2; Liu, Jinghua; Liu, Aihua1; Deng, Peng1; Zhang, Lin1; Gong, Xiaowei1; Zhao, Kesen1; Zhang, Shuangquan4; Jiang, Yong1
关键词Leukocyte-endothelial cell interaction Adhesion molecule Oligosaccharide Integrin Burn shock
刊名GLYCOCONJUGATE JOURNAL
2009-02-01
DOI10.1007/s10719-008-9174-0
26期:2页:173-188
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者National Key Basic Research Program ( 973 Program) of China ; Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) ; National Natural Science Foundation of China ; National Key Basic Research Program ( 973 Program) of China ; Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) ; National Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]SIALYL-LEWIS-X ; MICROVASCULAR ENDOTHELIAL-CELLS ; BINDING LECTIN SITE ; L-SELECTIN LIGAND ; P-SELECTIN ; ADHESION RECEPTORS ; THERMAL-INJURY ; T-CELLS ; IDENTIFICATION ; METASTASIS
英文摘要

Severe burn shock remains an unsolved clinical problem with urgent needs to explore novel therapeutic approaches. In this study, the in vivo bioactivity of a series of synthetic lactosyl derivatives ( oligosaccharides) was assessed on rats with burn shock to elucidate the underlying mechanisms. Administration of An-2 and Gu-4, two lactosyl derivatives with di- and tetravalent beta-D-galactopyranosyl(1-4)-beta-D-glucopyranosyl ligands, significantly prolonged the survival time (P<0.05 vs. saline), stabilized blood pressure and ameliorated the injuries to vital organs after burn. Flow chamber assay displayed that An-2 and Gu-4 markedly decreased the adhesion of leukocytes to microvessel endothelial cells. Competitive binding assay showed that a CD11b antibody significantly interrupted the interaction of An-2 and Gu-4 with leukocytes from rats with burn shock. With fluorescent microscopy, we further found that the oligosaccharides were selectively bound to leukocytes and with a colocalization of CD11b on the cell membrane. Interestingly, the lectin domain-deficient form of CD11b failed to bind with An-2 and Gu-4. The results suggest that both An-2 and Gu-4 significantly inhibit the adhesion of leukocytes to endothelial cells by binding to CD11b and thereby exert protective effects on severe burn shock.

语种英语
所属项目编号2002CB513005 ; IRT0731 ; 30670828 ; 30572151 ; 20372003 ; 90713004
资助者National Key Basic Research Program ( 973 Program) of China ; Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) ; National Natural Science Foundation of China ; National Key Basic Research Program ( 973 Program) of China ; Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) ; National Natural Science Foundation of China
WOS记录号WOS:000263417500005
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63026
专题北京大学药学院_化学生物学系
作者单位1.So Med Univ, Dept Pathophysiol, Key Lab Funct Prote Guangdong Prov, Guangzhou 510515, Guangdong, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Naval Hosp 411 PLA, Dept Surg, Shanghai 200081, Peoples R China
4.Nanjing Normal Univ, Coll Life Sci, Jiangsu Prov Key Lab Mol & Med Biotechnol, Nanjing 210046, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Zhihui,Li, Qing,Hu, Jiale,et al. Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes[J]. GLYCOCONJUGATE JOURNAL,2009,26(2):173-188.
APA Zhao, Zhihui.,Li, Qing.,Hu, Jiale.,Li, Zhongjun.,Liu, Jinghua.,...&Jiang, Yong.(2009).Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes.GLYCOCONJUGATE JOURNAL,26(2),173-188.
MLA Zhao, Zhihui,et al."Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes".GLYCOCONJUGATE JOURNAL 26.2(2009):173-188.
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