IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement
Liu, Wentao1,2; Wei, Yan1; Zhang, Xuehui3,5; Xu, Mingming1,5; Yang, Xiaoping4; Deng, Xuliang1,5
关键词bone tissue engineering mesenchymal stem cells nanofibers osteogenic differentiation global gene expression
刊名ACS NANO
2013-08-01
DOI10.1021/nn402118s
7期:8页:6928-6938
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Chemistry ; Science & Technology - Other Topics ; Materials Science
关键词[WOS]MESENCHYMAL STEM-CELLS ; CONTACT GUIDANCE ; OSTEOPROGENITOR RESPONSE ; DIFFERENTIATION ; BONE ; MARROW ; MATRIX ; PHENOTYPE ; ADHESION ; TISSUE
英文摘要

Nanotopographic cues from biomaterials exert powerful effects on the osteogenic differentiation of mesenchymal stem cells because of their niche-mimicking features. However, the biological mechanisms underlying cell lineage determination by surface nanotopography have not been clearly elucidated. Here, we explored the osteogenic behavior of human bone marrow mesenchymal stem cells (hBMSCs) on poly-L-lactide nanofibers with different orientations and monitored the dynamic changes in global gene expression triggered by topographical cues. RT-PCR analysis of osteogenic marker genes and ALP activity assays demonstrated that hBMSCs cultured on random nanofibers showed enhanced osteogenic-specific fate compared with those on aligned nanofibers. Microarray analysis demonstrated a similar temporal change in gene expression patterns between hBMSCs cultured on random nanofibers and those induced with an osteogenic supplement (OS). However, the extent of osteogenic differentiation on the fibrous scaffold was much lower than that driven by chemical OS. In-depth pathway analysis revealed that focal adhesion kinase, TGF-beta, Wnt, and MAPK pathways were involved in the activation of osteogenic differentiation in hBMSCs on random nanofibers. These findings suggested that a lower extent but similar rhythm of dynamic cellular behavior was induced on random nanofibers when compared with the OS condition and that mechanotransduction could trigger nonspecific and multilevel responses in hBMSCs. This study provides insight into the regulation of osteogenesis directed by substratum surfaces.

语种英语
WOS记录号WOS:000323810600054
项目编号2012CB933900 ; 81171000 ; 2012AA022501
资助机构National Basic Research Program of China ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Peking University-Tsinghua University Joint Center for Life Sciences
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63032
专题北京大学口腔医学院
北京大学口腔医学院_特诊科
北京大学临床肿瘤学院_乳腺癌预防治疗中心
作者单位1.Peking Univ Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
2.Jilin Univ, Key Lab Zoonosis, Minist Educ, Coll Vet Med, Changchun 130062, Peoples R China
3.Tsinghua Univ, Dept Mat Sci & Engn, Beijing 100084, Peoples R China
4.Beijing Univ Chem Technol, Minist Educ, Key Lab Carbon Fiber & Funct Polymers, Beijing 100029, Peoples R China
5.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Liu, Wentao,Wei, Yan,Zhang, Xuehui,et al. Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement[J]. ACS NANO,2013,7(8):6928-6938.
APA Liu, Wentao,Wei, Yan,Zhang, Xuehui,Xu, Mingming,Yang, Xiaoping,&Deng, Xuliang.(2013).Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement.ACS NANO,7(8),6928-6938.
MLA Liu, Wentao,et al."Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement".ACS NANO 7.8(2013):6928-6938.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Wentao]的文章
[Wei, Yan]的文章
[Zhang, Xuehui]的文章
百度学术
百度学术中相似的文章
[Liu, Wentao]的文章
[Wei, Yan]的文章
[Zhang, Xuehui]的文章
必应学术
必应学术中相似的文章
[Liu, Wentao]的文章
[Wei, Yan]的文章
[Zhang, Xuehui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。