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学科主题: 口腔医学
题名:
Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement
作者: Liu, Wentao1,2; Wei, Yan1; Zhang, Xuehui3,5; Xu, Mingming1,5; Yang, Xiaoping4; Deng, Xuliang1,5
关键词: bone tissue engineering ; mesenchymal stem cells ; nanofibers ; osteogenic differentiation ; global gene expression
刊名: ACS NANO
发表日期: 2013-08-01
DOI: 10.1021/nn402118s
卷: 7, 期:8, 页:6928-6938
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ; Science & Technology - Other Topics ; Materials Science
关键词[WOS]: MESENCHYMAL STEM-CELLS ; CONTACT GUIDANCE ; OSTEOPROGENITOR RESPONSE ; DIFFERENTIATION ; BONE ; MARROW ; MATRIX ; PHENOTYPE ; ADHESION ; TISSUE
英文摘要:

Nanotopographic cues from biomaterials exert powerful effects on the osteogenic differentiation of mesenchymal stem cells because of their niche-mimicking features. However, the biological mechanisms underlying cell lineage determination by surface nanotopography have not been clearly elucidated. Here, we explored the osteogenic behavior of human bone marrow mesenchymal stem cells (hBMSCs) on poly-L-lactide nanofibers with different orientations and monitored the dynamic changes in global gene expression triggered by topographical cues. RT-PCR analysis of osteogenic marker genes and ALP activity assays demonstrated that hBMSCs cultured on random nanofibers showed enhanced osteogenic-specific fate compared with those on aligned nanofibers. Microarray analysis demonstrated a similar temporal change in gene expression patterns between hBMSCs cultured on random nanofibers and those induced with an osteogenic supplement (OS). However, the extent of osteogenic differentiation on the fibrous scaffold was much lower than that driven by chemical OS. In-depth pathway analysis revealed that focal adhesion kinase, TGF-beta, Wnt, and MAPK pathways were involved in the activation of osteogenic differentiation in hBMSCs on random nanofibers. These findings suggested that a lower extent but similar rhythm of dynamic cellular behavior was induced on random nanofibers when compared with the OS condition and that mechanotransduction could trigger nonspecific and multilevel responses in hBMSCs. This study provides insight into the regulation of osteogenesis directed by substratum surfaces.

语种: 英语
所属项目编号: 2012CB933900 ; 81171000 ; 2012AA022501
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China ; Peking University-Tsinghua University Joint Center for Life Sciences
WOS记录号: WOS:000323810600054
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63032
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
2.Jilin Univ, Key Lab Zoonosis, Minist Educ, Coll Vet Med, Changchun 130062, Peoples R China
3.Tsinghua Univ, Dept Mat Sci & Engn, Beijing 100084, Peoples R China
4.Beijing Univ Chem Technol, Minist Educ, Key Lab Carbon Fiber & Funct Polymers, Beijing 100029, Peoples R China
5.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Wentao,Wei, Yan,Zhang, Xuehui,et al. Lower Extent but Similar Rhythm of Osteogenic Behavior in hBMSCs Cultured on Nanofibrous Scaffolds versus Induced with Osteogenic Supplement[J]. ACS NANO,2013,7(8):6928-6938.
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