IR@PKUHSC  > 北京大学第三临床医学院  > 泌尿外科
学科主题临床医学
A bladder cancer microenvironment simulation system based on a microfluidic co-culture model
Liu, Peng-fei1,2; Cao, Yan-wei1,3; Zhang, Shu-dong4; Zhao, Yang1,2; Liu, Xiao-guang1,2; Shi, Hao-qing1,2; Hu, Ke-yao1,2; Zhu, Guan-qun1,2; Ma, Bo5,6; Niu, Hai-tao1,3
关键词microfluidic device bladder cancer microenvironment three-dimensional culture co-culture drug sensitivity
刊名ONCOTARGET
2015-11-10
6期:35页:37695-37705
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Cell Biology
资助者National Natural Science Foundation of China ; Wu JiePing Medical Foundation ; Natural Science Foundation of Shandong Province, China ; National Natural Science Foundation of China ; Wu JiePing Medical Foundation ; Natural Science Foundation of Shandong Province, China
研究领域[WOS]Oncology ; Cell Biology
关键词[WOS]TUMOR MICROENVIRONMENT ; BREAST-CANCER ; ANTICANCER THERAPIES ; VASCULOGENIC MIMICRY ; ANGIOGENIC SWITCH ; DRUG-RESISTANCE ; LUNG-CANCER ; CELLS ; HETEROGENEITY ; EXPRESSION
英文摘要

A tumor microenvironment may promote tumor metastasis and progression through the dynamic interplay between neoplastic cells and stromal cells. In this work, the most representative and significant stromal cells, fibroblasts, endothelial cells, and macrophages were used as vital component elements and combined with bladder cancer cells to construct a bladder cancer microenvironment simulation system. This is the first report to explore bladder cancer microenvironments based on 4 types of cells co-cultured simultaneously. This simulation system comprises perfusion equipment, matrigel channel units, a medium channel and four indirect contact culture chambers, allowing four types of cells to simultaneously interact through soluble biological factors and metabolites. With this system, bladder cancer cells (T24) with a tendency to form a ′reticular′ structure under 3 dimensional culture conditions were observed in real time. The microenvironment characteristics of paracrine interactions and cell motility were successfully simulated in this system. The phenotype change process in stromal cells was successfully reproduced in this system by testing the macrophage effector molecule Arg-1. Arg-1 was highly expressed in the simulated tumor microenvironment group. To develop "precision medicine" in bladder cancer therapy, bladder cancer cells were treated with different clinical ′neo-adjuvant′ chemotherapy schemes in this system, and their sensitivity differences were fully reflected. This work provides a preliminary foundation for neo-adjuvant chemotherapy in bladder cancer, a theoretical foundation for tumor microenvironment simulation and promotes individual therapy in bladder cancer patients.

语种英语
所属项目编号30901481 ; 81372752 ; 81472411 ; 320.6750.13261 ; ZR2014HM088
资助者National Natural Science Foundation of China ; Wu JiePing Medical Foundation ; Natural Science Foundation of Shandong Province, China ; National Natural Science Foundation of China ; Wu JiePing Medical Foundation ; Natural Science Foundation of Shandong Province, China
WOS记录号WOS:000366113200055
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63033
专题北京大学第三临床医学院_泌尿外科
作者单位1.Qingdao Univ, Affiliated Hosp, Dept Urol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Univ, Coll Med, Qingdao 266071, Shandong, Peoples R China
3.Key Urol Lab Qingdao City, Qingdao, Shandong, Peoples R China
4.Peking Univ, Hosp 3, Dept Urol, Beijing 100871, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China
6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Liu, Peng-fei,Cao, Yan-wei,Zhang, Shu-dong,et al. A bladder cancer microenvironment simulation system based on a microfluidic co-culture model[J]. ONCOTARGET,2015,6(35):37695-37705.
APA Liu, Peng-fei.,Cao, Yan-wei.,Zhang, Shu-dong.,Zhao, Yang.,Liu, Xiao-guang.,...&Niu, Hai-tao.(2015).A bladder cancer microenvironment simulation system based on a microfluidic co-culture model.ONCOTARGET,6(35),37695-37705.
MLA Liu, Peng-fei,et al."A bladder cancer microenvironment simulation system based on a microfluidic co-culture model".ONCOTARGET 6.35(2015):37695-37705.
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