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学科主题: 临床医学
题名:
A bladder cancer microenvironment simulation system based on a microfluidic co-culture model
作者: Liu, Peng-fei1,2; Cao, Yan-wei1,3; Zhang, Shu-dong4; Zhao, Yang1,2; Liu, Xiao-guang1,2; Shi, Hao-qing1,2; Hu, Ke-yao1,2; Zhu, Guan-qun1,2; Ma, Bo5,6; Niu, Hai-tao1,3
关键词: microfluidic device ; bladder cancer ; microenvironment ; three-dimensional culture ; co-culture ; drug sensitivity
刊名: ONCOTARGET
发表日期: 2015-11-10
卷: 6, 期:35, 页:37695-37705
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: TUMOR MICROENVIRONMENT ; BREAST-CANCER ; ANTICANCER THERAPIES ; VASCULOGENIC MIMICRY ; ANGIOGENIC SWITCH ; DRUG-RESISTANCE ; LUNG-CANCER ; CELLS ; HETEROGENEITY ; EXPRESSION
英文摘要:

A tumor microenvironment may promote tumor metastasis and progression through the dynamic interplay between neoplastic cells and stromal cells. In this work, the most representative and significant stromal cells, fibroblasts, endothelial cells, and macrophages were used as vital component elements and combined with bladder cancer cells to construct a bladder cancer microenvironment simulation system. This is the first report to explore bladder cancer microenvironments based on 4 types of cells co-cultured simultaneously. This simulation system comprises perfusion equipment, matrigel channel units, a medium channel and four indirect contact culture chambers, allowing four types of cells to simultaneously interact through soluble biological factors and metabolites. With this system, bladder cancer cells (T24) with a tendency to form a ′reticular′ structure under 3 dimensional culture conditions were observed in real time. The microenvironment characteristics of paracrine interactions and cell motility were successfully simulated in this system. The phenotype change process in stromal cells was successfully reproduced in this system by testing the macrophage effector molecule Arg-1. Arg-1 was highly expressed in the simulated tumor microenvironment group. To develop "precision medicine" in bladder cancer therapy, bladder cancer cells were treated with different clinical ′neo-adjuvant′ chemotherapy schemes in this system, and their sensitivity differences were fully reflected. This work provides a preliminary foundation for neo-adjuvant chemotherapy in bladder cancer, a theoretical foundation for tumor microenvironment simulation and promotes individual therapy in bladder cancer patients.

语种: 英语
所属项目编号: 30901481 ; 81372752 ; 81472411 ; 320.6750.13261 ; ZR2014HM088
项目资助者: National Natural Science Foundation of China ; Wu JiePing Medical Foundation ; Natural Science Foundation of Shandong Province, China
WOS记录号: WOS:000366113200055
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63033
Appears in Collections:北京大学第三临床医学院_泌尿外科_期刊论文

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作者单位: 1.Qingdao Univ, Affiliated Hosp, Dept Urol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Univ, Coll Med, Qingdao 266071, Shandong, Peoples R China
3.Key Urol Lab Qingdao City, Qingdao, Shandong, Peoples R China
4.Peking Univ, Hosp 3, Dept Urol, Beijing 100871, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao, Shandong, Peoples R China
6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China

Recommended Citation:
Liu, Peng-fei,Cao, Yan-wei,Zhang, Shu-dong,et al. A bladder cancer microenvironment simulation system based on a microfluidic co-culture model[J]. ONCOTARGET,2015,6(35):37695-37705.
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