北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
Ischemic injury underlies the pathogenesis of aristolochic acid-induced acute kidney injury
作者: Wen, Yu-Jie; Qu, Lei; Li, Xiao-Mei
刊名: TRANSLATIONAL RESEARCH
发表日期: 2008-07-01
DOI: 10.1016/j.trsl.2008.05.002
卷: 152, 期:1, 页:38-46
收录类别: ISTP ; SCI
文章类型: Proceedings Paper
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology ; Medicine, General & Internal ; Medicine, Research & Experimental
研究领域[WOS]: Medical Laboratory Technology ; General & Internal Medicine ; Research & Experimental Medicine
关键词[WOS]: CHINESE HERBS NEPHROPATHY ; ENDOTHELIAL GROWTH-FACTOR ; INTERSTITIAL RENAL FIBROSIS ; EPITHELIAL-CELLS ; IN-VIVO ; HYPOXIA ; EXPRESSION ; FAILURE ; PATHWAY ; APOPTOSIS
英文摘要:

Aristolochic acid nephropathy (AAN) is a Progressive tubulointerstitial renal disease caused by aristolochic acid intake. To determine the contribution of renal ischemia to the pathogenesis of AAN, we characterized changes in the expression of angiogenic factors and vasoactive substances, and then we evaluated the expression of a marker of hypoxia in an acute AAN rat model. Rats were orally administrated either a decoction of Aristolochiae manshuriensis that contained 20 mg/kg of aristolochic acid-l or an equal volume of distilled water (control group) once daily for 4 days or 7 days. Renal histology and serum creatinine were assessed. Expression of endothelin-1 (ET-1) and hypoxia inducible factor-1 alpha (HIF-1 alpha) mRNA within renal cortex were determined by semiquantitative reverse-transcription polymerase chain reaction. Levels of ET-1, nitric oxide (NO), vascular endothelial growth factor (VEGF), and HIF-1 alpha in kidneys were determined by radioimmunoassay, Griess method, Western blot, and immunohistochemistry, respectively. Tubular injury scores and ET-1 mRNA expression were increased in the AA-treated rats at both days 4 and 8, whereas serum creatinine level and ET-1 protein expression was increased only at day 4. In contrast, NO production in AA-treated rats was decreased at day 8 compared with the control group. Similarly, VEGF protein expression was reduced in the AA-treated rats at both days 4 and 8. A dramatic increase in nuclear staining for HIF-1 alpha was observed mainly in the tubular cells of tubulointerstitial damage area in the AA-treated rats at day 8. The observed increase in HIF-1 alpha protein expression, decrease in VEGF protein expression, and imbalance of vasoactive substances after induction of acute kidney injury by AA suggests that ischemic injury contributes to the pathogenesis of AAN.

语种: 英语
WOS记录号: WOS:000257695100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63076
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Div Renal, Dept Med,Minist Hlth China, First Hosp,Inst Nephrol,Key Lab Renal Dis, Beijing 100034, Peoples R China

Recommended Citation:
Wen, Yu-Jie,Qu, Lei,Li, Xiao-Mei. Ischemic injury underlies the pathogenesis of aristolochic acid-induced acute kidney injury[J]. TRANSLATIONAL RESEARCH,2008,152(1):38-46.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wen, Yu-Jie]'s Articles
[Qu, Lei]'s Articles
[Li, Xiao-Mei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wen, Yu-Jie]‘s Articles
[Qu, Lei]‘s Articles
[Li, Xiao-Mei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace