IR@PKUHSC  > 北京大学基础医学院  > 放射医学教研室
学科主题基础医学
Mechanism underlying carbon tetrachloride-inhibited protein synthesis in liver
Li, Xiao-Wen2; Zhu, Rong3; Li, Bo1; Zhou, Mei1; Sheng, Qing-Jian1; Yang, Ye-Peng1; Han, Nan-Yin4; Li, Zai-Quan1
关键词Carbon tetrachloride Nuclear envelope nucleotide triphosphatase Nucleocytoplasmic transport inhibition Hydroxyl radical
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2010-08-21
DOI10.3748/wjg.v16.i31.3950
16期:31页:3950-3956
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]ENVELOPE NUCLEOSIDE TRIPHOSPHATASE ; MESSENGER-RNA EXPORT ; NUCLEAR ; INVOLVEMENT ; CELLS ; MICE
英文摘要

AIM: To study the mechanism underlying carbon tetrachloride (CCl(4)) -induced alterations of protein synthesis in liver.

METHODS: Male Sprague-Dawley rats were given CCl(4) (1 mL/100 g body weight) and (3)H-leucine incorporation. Malondialdehyde (MDA) level in the liver, in vitro response of hepatocyte nuclei nucleotide triphosphatase (NTPase) to free radicals, and nuclear export of total mRNA with 3′-poly A(+) were measured respectively. Survival response of HepG2 cells to CCl(4) treatment was assessed by methyl thiazolyl tetrazolium. Km and Vmax values of nuclear envelope NTPase activity in liver of rats treated with CCl(4) were assayed by a double-reciprocal plot.

RESULTS: The protein synthesis was inhibited while the MDA level was significantly increased in liver of rats treated with CCl(4). In addition, CCl(4) decreased the NTPase binding capacity of nuclear envelope (Km value) in cultured HepG2 cells. Moreover, in vitro ferrous radicals from Fenton′s system suppressed the NTPase activity of liver nuclear envelope in a dose-dependent manner. Down-regulation of the nuclear envelope NTPase activity indicated a lower energy provision for nucleocytoplasmic transport of mRNA molecules, an evidence in CCl(4)-treated HepG2 cells correspondingly supported by the nuclear sequestration of poly (A)(+) mRNA molecules in morphological hybridization research.

CONCLUSION: Inhibition of mRNA transport, suggestive of decreased NTPase activity of the nuclear envelope, may be involved in carbon tetrachloride-inhibited protein synthesis in liver. (C) 2010 Baishideng. All rights reserved.

语种英语
所属项目编号30470846
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000281118000013
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63077
Collection北京大学基础医学院_放射医学教研室
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Radiat Med, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Natl Inst Drug Dependence, Beijing 100191, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiao-Wen,Zhu, Rong,Li, Bo,et al. Mechanism underlying carbon tetrachloride-inhibited protein synthesis in liver[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2010,16(31):3950-3956.
APA Li, Xiao-Wen.,Zhu, Rong.,Li, Bo.,Zhou, Mei.,Sheng, Qing-Jian.,...&Li, Zai-Quan.(2010).Mechanism underlying carbon tetrachloride-inhibited protein synthesis in liver.WORLD JOURNAL OF GASTROENTEROLOGY,16(31),3950-3956.
MLA Li, Xiao-Wen,et al."Mechanism underlying carbon tetrachloride-inhibited protein synthesis in liver".WORLD JOURNAL OF GASTROENTEROLOGY 16.31(2010):3950-3956.
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