|Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva|
|Pan, Yan1; Iwata, Fusako2; Wang, Di1; Muraguchi, Masahiro2; Ooga, Keiko2; Ohmoto, Yasukazu2; Takai, Masaaki2; Cho, Gota1; Kang, Jinsen1; Shono, Masayuki3; Li, Xue-jun4; Okamura, Ko5; Mori, Toyoki2; Ishikawa, Yasuko1|
|关键词||Aquaporin-5 Water channel Lipid rafts Human saliva Biomarker Salivary flow rate Anti-human AQP5-antibody|
|刊名||BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS|
|WOS标题词||Science & Technology|
|类目[WOS]||Biochemistry & Molecular Biology ; Biophysics|
|研究领域[WOS]||Biochemistry & Molecular Biology ; Biophysics|
|关键词[WOS]||APICAL PLASMA-MEMBRANE ; RAT PAROTID-GLANDS ; WATER CHANNEL ; URINARY-EXCRETION ; SIGNAL-TRANSDUCTION ; AMYLASE SECRETION ; ACINAR-CELLS ; TRAFFICKING ; ACETYLCHOLINE ; TRANSLOCATION|
Background: It is unknown whether AQP5 and lipid rafts are released into human unstimulated (resting) saliva and saliva in response to secretagogues.
Methods: In order to quantitate the salivary concentration of AQP5, we produced a polyclonal antibody for human AQP5 and developed an enzyme-like immunosorbent assay (ELISA).
Results: AQP5 and lipid rafts were identified in human resting saliva. The amount of AQP5 in resting saliva showed a diurnal variation with high levels during waking hours, and an age-related decrease in AQP5 was coincident with the volume of resting saliva. Cevimeline, a muscarinic acetylcholine receptor (mAChR) agonist, induced the release of AQP5 with lipid rafts, amylase, mucin, and lysozyme. Changes in saliva AQP5 levels after cevimeline administration occurred simultaneously with changes in saliva flow rates. Confocal microscopy revealed that AQP5 was located in the apical plasma membrane and showed a diffuse pattern in parotid glands under resting conditions. Following cevimeline administration, AQP5 was predominantly associated with the APM and was localized in the lumen.
General significance: AQP5 and lipid rafts were released with salivary proteins from human salivary glands by the stimulation Of M-3 mAChRs, and that changes in saliva AQP5 levels can be used as an indicator of salivary flow rate and also as a useful index Of M3 mAChR agonist′s action on human salivary glands. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
|资助机构||Ministry of Education, Culture, Sports, Science, and Technology of Japan ; Research Grant from the Daiichi Pharmaceutical Co. (Tokyo, Japan)|
|作者单位||1.Univ Tokushima, Dept Pharmacol, Grad Sch Dent, Tokushima 7708504, Japan|
2.Otsuka Pharmaceut Co Ltd, Res Inst Pharmacol & Therapeut Dev, Tokushima 7710192, Japan
3.Univ Tokushima, Support Ctr Adv Med Sci, Tokushima 7708504, Japan
4.Peking Univ, Dept Pharmacol, Hlth Sci Ctr, Beijing 100191, Peoples R China
5.Osaka Univ, Grad Sch Med, Dept Social & Environm Med, Suita, Osaka 5650871, Japan
|Pan, Yan,Iwata, Fusako,Wang, Di,et al. Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2009,1790(1):49-56.|
|APA||Pan, Yan.,Iwata, Fusako.,Wang, Di.,Muraguchi, Masahiro.,Ooga, Keiko.,...&Ishikawa, Yasuko.(2009).Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1790(1),49-56.|
|MLA||Pan, Yan,et al."Identification of aquaporin-5 and lipid rafts in human resting saliva and their release into cevimeline-stimulated saliva".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1790.1(2009):49-56.|
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