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学科主题口腔医学
Beyond Toll-Like Receptors: Porphyromonas gingivalis Induces IL-6, IL-8, and VCAM-1 Expression Through NOD-Mediated NF-kappa B and ERK Signaling Pathways in Periodontal Fibroblasts
Liu, Jianru1; Wang, Yixiang2; Ouyang, Xiangying1
关键词NOD1/2 VCAM-1 Periodontal fibroblasts NF-kappa B ERK1/2
刊名INFLAMMATION
2014-04-01
DOI10.1007/s10753-013-9766-0
37期:2页:522-533
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]PATTERN-RECOGNITION RECEPTORS ; CELL-ADHESION MOLECULES ; INNATE IMMUNE-RESPONSES ; ENDOTHELIAL-CELLS ; LIGAMENT FIBROBLASTS ; CREVICULAR FLUID ; UP-REGULATION ; ACTIVATION ; CYTOKINE ; DISEASE
英文摘要

To investigate whether oligomerization domains (NODs) are involved in Porphyromonas gingivalis-induced interleukin (IL)-6, IL-8, and vascular cell adhesion molecule (VCAM)-1 expression beyond Toll-like receptors (TLRs), we investigated the role of NOD1/2 in P. gingivalis-induced IL-6, IL-8, and VCAM-1 expression in human gingival fibroblasts (hGFs) and periodontal ligament cells (hPDLCs). The mechanism was explored by activation and silence of NODs, electrophoretic mobility shift assay (EMSA), and pathway blockade assays. Results showed that P. gingivalis could induce NOD1, NOD2, IL-6, IL-8, and VCAM-1 expression in hGFs and hPDLs at mRNA and protein levels. Activation of NOD1/2 by agonists could clearly upregulate the expression of these genes, while silence of NOD1/2 could remarkably attenuate them. EMSA and blockade of NF-kappa B and extracellular-signal-regulated kinase (ERK)1/2 pathway assays also verified that the two pathways were involved in NOD1/2-mediated IL-6, IL-8, and VCAM-1 expression. In conclusion, our findings demonstrated that P. gingivalis induced IL-6, IL-8, and VCAM-1 expression in hGFs and hPDLCs through NOD1/2-mediated NF-kappa B and ERK1/2 signaling pathways beyond TLRs.

语种英语
所属项目编号181271150
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000332715000027
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63085
专题北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
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GB/T 7714
Liu, Jianru,Wang, Yixiang,Ouyang, Xiangying. Beyond Toll-Like Receptors: Porphyromonas gingivalis Induces IL-6, IL-8, and VCAM-1 Expression Through NOD-Mediated NF-kappa B and ERK Signaling Pathways in Periodontal Fibroblasts[J]. INFLAMMATION,2014,37(2):522-533.
APA Liu, Jianru,Wang, Yixiang,&Ouyang, Xiangying.(2014).Beyond Toll-Like Receptors: Porphyromonas gingivalis Induces IL-6, IL-8, and VCAM-1 Expression Through NOD-Mediated NF-kappa B and ERK Signaling Pathways in Periodontal Fibroblasts.INFLAMMATION,37(2),522-533.
MLA Liu, Jianru,et al."Beyond Toll-Like Receptors: Porphyromonas gingivalis Induces IL-6, IL-8, and VCAM-1 Expression Through NOD-Mediated NF-kappa B and ERK Signaling Pathways in Periodontal Fibroblasts".INFLAMMATION 37.2(2014):522-533.
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