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学科主题: 口腔医学
题名:
Beyond Toll-Like Receptors: Porphyromonas gingivalis Induces IL-6, IL-8, and VCAM-1 Expression Through NOD-Mediated NF-kappa B and ERK Signaling Pathways in Periodontal Fibroblasts
作者: Liu, Jianru1; Wang, Yixiang2; Ouyang, Xiangying1
关键词: NOD1/2 ; VCAM-1 ; Periodontal fibroblasts ; NF-kappa B ; ERK1/2
刊名: INFLAMMATION
发表日期: 2014-04-01
DOI: 10.1007/s10753-013-9766-0
卷: 37, 期:2, 页:522-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: PATTERN-RECOGNITION RECEPTORS ; CELL-ADHESION MOLECULES ; INNATE IMMUNE-RESPONSES ; ENDOTHELIAL-CELLS ; LIGAMENT FIBROBLASTS ; CREVICULAR FLUID ; UP-REGULATION ; ACTIVATION ; CYTOKINE ; DISEASE
英文摘要:

To investigate whether oligomerization domains (NODs) are involved in Porphyromonas gingivalis-induced interleukin (IL)-6, IL-8, and vascular cell adhesion molecule (VCAM)-1 expression beyond Toll-like receptors (TLRs), we investigated the role of NOD1/2 in P. gingivalis-induced IL-6, IL-8, and VCAM-1 expression in human gingival fibroblasts (hGFs) and periodontal ligament cells (hPDLCs). The mechanism was explored by activation and silence of NODs, electrophoretic mobility shift assay (EMSA), and pathway blockade assays. Results showed that P. gingivalis could induce NOD1, NOD2, IL-6, IL-8, and VCAM-1 expression in hGFs and hPDLs at mRNA and protein levels. Activation of NOD1/2 by agonists could clearly upregulate the expression of these genes, while silence of NOD1/2 could remarkably attenuate them. EMSA and blockade of NF-kappa B and extracellular-signal-regulated kinase (ERK)1/2 pathway assays also verified that the two pathways were involved in NOD1/2-mediated IL-6, IL-8, and VCAM-1 expression. In conclusion, our findings demonstrated that P. gingivalis induced IL-6, IL-8, and VCAM-1 expression in hGFs and hPDLCs through NOD1/2-mediated NF-kappa B and ERK1/2 signaling pathways beyond TLRs.

语种: 英语
所属项目编号: 181271150
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000332715000027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63085
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Dept Periodontol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Jianru,Wang, Yixiang,Ouyang, Xiangying. Beyond Toll-Like Receptors: Porphyromonas gingivalis Induces IL-6, IL-8, and VCAM-1 Expression Through NOD-Mediated NF-kappa B and ERK Signaling Pathways in Periodontal Fibroblasts[J]. INFLAMMATION,2014,37(2):522-533.
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