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学科主题: 基础医学
题名:
Hypertriglyceridemia and delayed clearance of fat load in transgenic rabbits expressing human apolipoprotein CIII
作者: Ding, Yinyuan1,2; Wang, Yuhui1,2; Zhu, Hong3; Fan, Jianglin4; Yu, Liqing5; Liu, George1,2; Liu, Enqi3
关键词: Apolipoprotein CIII ; Transgenic rabbit ; Hypertriglyceridemia
刊名: TRANSGENIC RESEARCH
发表日期: 2011-08-01
DOI: 10.1007/s11248-010-9467-5
卷: 20, 期:4, 页:867-875
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]: DENSITY-LIPOPROTEIN CHOLESTEROL ; DIET-INDUCED OBESITY ; IV GENE-CLUSTER ; C-III ; APOC-III ; HYPERLIPOPROTEINEMIA FAILS ; ENDOTHELIAL-CELLS ; MONOCYTIC CELLS ; LIPASE ACTIVITY ; LIPID PROFILE
英文摘要:

Apolipoprotein CIII (apoCIII) has been implicated in hypertriglyceridemia and plasma apoCIII independently predicts risk for coronary heart disease. While hypertriglyceridemia in patients has been demonstrated to correlate with elevated plasma apoCIII levels and reduced lipoprotein lipase (LPL) activity, apoCIII transgenic mice show elevated LPL activity. In this study, we generated transgenic (Tg) rabbits expressing human apoCIII gene exclusively in liver and investigated the effect of apoCIII overexpression on lipid metabolism of rabbits. In comparison with non-Tg littermates, Tg rabbits had 3- and 3.2-fold increases in fed and fasted plasma triglycerides, respectively. In contrast, no significant differences were observed in plasma total cholesterol and high density lipoprotein cholesterol levels between Tg and non-Tg rabbits. Analysis of lipoprotein fractions revealed that elevated plasma triglyceride levels in Tg rabbits were mainly attributed to an increase in very low density lipoprotein/chylomicron-sized particles. Furthermore, Tg rabbits showed markedly delayed clearance of plasma triglycerides accompanied with significantly reduced LPL activity in post-heparin plasma compared to that in non-Tg controls. In conclusion, these results indicate apoCIII transgenic rabbits develop hypertriglyceridemia with similar mechanism in hypertriglyceridemic patients via delayed clearance of plasma triglycerides, and could be used as a valuable tool for the study of human hyperlipidemia in relation with atherosclerotic disorders.

语种: 英语
所属项目编号: 2006AA02Z119 ; 30821001 ; G2006CD503801 ; 5101004
项目资助者: National High Technology R&amp ; D Program of China (863 Program) ; National Natural Science Foundation of the People&prime ; s Republic of China ; Major National Basic Research Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000292880900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63095
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Inst Cardiovasc Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Key Lab Mol Cardiovasc Sci, Minist Educ, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.Xi An Jiao Tong Univ, Lab Anim Ctr, Xian 710061, Peoples R China
4.Univ Yamanashi, Dept Mol Pathol, Interdisciplinary Grad Sch Med & Engn, Yamanashi 4093898, Japan
5.Wake Forest Univ, Dept Pathol, Sect Lipid Sci, Sch Med, Winston Salem, NC 27157 USA

Recommended Citation:
Ding, Yinyuan,Wang, Yuhui,Zhu, Hong,et al. Hypertriglyceridemia and delayed clearance of fat load in transgenic rabbits expressing human apolipoprotein CIII[J]. TRANSGENIC RESEARCH,2011,20(4):867-875.
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