IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis
Zheng, Jing-gao1,2; Huo, Tiancheng1,2; Tian, Ning1,2; Chen, Tianyuan1,2; Wang, Chengming1,2; Zhang, Ning1,2; Zhao, Fengying1,2; Lu, Danyu3; Ma, Wanyun1,2; Sun, Jia-lin1,2; Xue, Ping1,2
关键词spindle scattering noninvasive full-field optical coherence tomography
刊名JOURNAL OF BIOMEDICAL OPTICS
2013-05-01
DOI10.1117/1.JBO.18.5.050505
18期:5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]OPTICAL COHERENCE TOMOGRAPHY ; MICROSCOPY ; OOCYTES ; EMBRYO
英文摘要

The spindle plays a crucial role in normal chromosome alignment and segregation during meiosis and mitosis. Studying spindles in living cells noninvasively is of great value in assisted reproduction technology (ART). Here, we present a novel spindle imaging methodology, full-field optical coherence tomography (FF-OCT). Without any dye labeling and fixation, we demonstrate the first successful application of FF-OCT to noninvasive three-dimensional (3-D) live imaging of the meiotic spindles within the mouse living oocytes at metaphase II as well as the mitotic spindles in the living zygotes at metaphase and telophase. By post-processing of the 3-D dataset obtained with FF-OCT, the important morphological and spatial parameters of the spindles, such as short and long axes, spatial localization, and the angle of meiotic spindle deviation from the first polar body in the oocyte were precisely measured with the spatial resolution of 0.7 mu m. Our results reveal the potential of FF-OCT as an imaging tool capable of noninvasive 3-D live morphological analysis for spindles, which might be useful to ART related procedures and many other spindle related studies. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.18.5.050505]

语种英语
WOS记录号WOS:000322339900005
项目编号2006AA02Z472 ; 2007CB511904 ; 001CB510307 ; 2009CB929400 ; 61227807 ; 20130002110079
资助机构Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Ministry of Education of China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/63128
专题北京大学基础医学院
作者单位1.Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China
2.Tsinghua Univ, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
3.Peking Univ, Dept Anat & Histol & Embryol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Jing-gao,Huo, Tiancheng,Tian, Ning,et al. Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis[J]. JOURNAL OF BIOMEDICAL OPTICS,2013,18(5).
APA Zheng, Jing-gao.,Huo, Tiancheng.,Tian, Ning.,Chen, Tianyuan.,Wang, Chengming.,...&Xue, Ping.(2013).Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis.JOURNAL OF BIOMEDICAL OPTICS,18(5).
MLA Zheng, Jing-gao,et al."Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis".JOURNAL OF BIOMEDICAL OPTICS 18.5(2013).
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