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学科主题: 基础医学
题名:
Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis
作者: Zheng, Jing-gao1,2; Huo, Tiancheng1,2; Tian, Ning1,2; Chen, Tianyuan1,2; Wang, Chengming1,2; Zhang, Ning1,2; Zhao, Fengying1,2; Lu, Danyu3; Ma, Wanyun1,2; Sun, Jia-lin1,2; Xue, Ping1,2
关键词: spindle ; scattering ; noninvasive ; full-field optical coherence tomography
刊名: JOURNAL OF BIOMEDICAL OPTICS
发表日期: 2013-05-01
DOI: 10.1117/1.JBO.18.5.050505
卷: 18, 期:5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: OPTICAL COHERENCE TOMOGRAPHY ; MICROSCOPY ; OOCYTES ; EMBRYO
英文摘要:

The spindle plays a crucial role in normal chromosome alignment and segregation during meiosis and mitosis. Studying spindles in living cells noninvasively is of great value in assisted reproduction technology (ART). Here, we present a novel spindle imaging methodology, full-field optical coherence tomography (FF-OCT). Without any dye labeling and fixation, we demonstrate the first successful application of FF-OCT to noninvasive three-dimensional (3-D) live imaging of the meiotic spindles within the mouse living oocytes at metaphase II as well as the mitotic spindles in the living zygotes at metaphase and telophase. By post-processing of the 3-D dataset obtained with FF-OCT, the important morphological and spatial parameters of the spindles, such as short and long axes, spatial localization, and the angle of meiotic spindle deviation from the first polar body in the oocyte were precisely measured with the spatial resolution of 0.7 mu m. Our results reveal the potential of FF-OCT as an imaging tool capable of noninvasive 3-D live morphological analysis for spindles, which might be useful to ART related procedures and many other spindle related studies. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.18.5.050505]

语种: 英语
所属项目编号: 2006AA02Z472 ; 2007CB511904 ; 001CB510307 ; 2009CB929400 ; 61227807 ; 20130002110079
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Ministry of Education of China
WOS记录号: WOS:000322339900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63128
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China
2.Tsinghua Univ, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
3.Peking Univ, Dept Anat & Histol & Embryol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zheng, Jing-gao,Huo, Tiancheng,Tian, Ning,et al. Noninvasive three-dimensional live imaging methodology for the spindles at meiosis and mitosis[J]. JOURNAL OF BIOMEDICAL OPTICS,2013,18(5).
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