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学科主题: 基础医学
题名:
Sorafenib suppresses the epithelial-mesenchymal transition of hepatocellular carcinoma cells after insufficient radiofrequency ablation
作者: Dong, Shuying1; Kong, Jian1; Kong, Fandong5,6; Kong, Jinge5,6; Gao, Jun1; Ji, Liang2,3,4; Pan, Bing2,3,4; Chen, Lian7; Zheng, Lemin2,3,4; Sun, Wenbing1
关键词: Hepatocellular carcinoma ; Insufficient radiofrequency ablation ; Epithelial-mesenchymal transition ; Sorafenib
刊名: BMC CANCER
发表日期: 2015-11-30
DOI: 10.1186/s12885-015-1949-7
卷: 15
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: RAPID PROGRESSION ; PATHWAYS ; CHEMOEMBOLIZATION ; METASTASIS ; INHIBITION ; THERAPY ; CANCER
英文摘要:

Background: Epithelial-mesenchymal transition (EMT) played an important role in the progression of hepatocellular carcinoma (HCC) after insufficient radiofrequency ablation (RFA). However, whether sorafenib could be used to suppress the EMT of HCC after insufficient RFA and further prevent the progression of residual HCC remains poorly unknown.

Methods: Insufficient RFA was simulated using a water bath (47 degrees C 5, 10, 15, 20 and 25 min gradually). MTT assay and transwell assay were used to evaluate the effects of sorafenib on viability, migration and invasion of HepG2 and SMMC7721 cells after insufficient RFA in vitro. After insufficient RFA, the molecular changes in HCC cells with the treatment of sorafeinb were evaluated using western blot and ELISAs. An ectopic nude mice model was used to evaluate the effect of sorafenib on the growth of HepG2 cells in vivo after insufficient RFA.

Results: HepG2 and SMMC7721 cells after insufficient RFA (named as HepG2-H and SMMC7721-H) exhibited enhanced viability, migration and invasion in vitro. Sorafenib inhibited the enhanced viability, migration and invasion of HepG2 and SMMC7721 cells after insufficient RFA. Molecular changes of EMT were observed in HepG2-H and SMMC7721-H cells. Sorafenib inhibited the EMT of HepG2-H and SMMC7721-H cells. HepG2-H cells also exhibited larger tumor size in vivo. Higher expression of PCNA, Ki67, N-cadherin, MMP-2 and MMP-9, was also observed in HepG2-H tumors. Sorafenib blocked the enhanced growth of HepG2 cells in vivo after insufficient RFA.

Conclusions: Sorafenib inhibited the EMT of HCC cells after insufficient RFA, and may be used to prevent the progression of HCC after RFA.

语种: 英语
所属项目编号: 320675007131 ; 32067501207 ; 2011CB503900 ; 81170101 ; 81370235 ; 81572957 ; 81502650 ; 7122106
项目资助者: Dr. Jieping Wu Medical Foundation ; Program for Medical Key Discipline of Shijingshan District, Beijing ; "973" National ST Major Project ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing, China
WOS记录号: WOS:000365490400001
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/63140
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Chao Yang Hosp, Dept Hepatobiliary Surg, Beijing 100043, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Minist Educ,Inst Cardiovasc Sci, Beijing 100191, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Inst Syst Biomed, Beijing 100191, Peoples R China
5.Peking Univ, Neurosci Res Inst, Sch Basic Med Sci, Dept Neurobiol, Beijing 100191, Peoples R China
6.Peking Univ, Natl Hlth & Family Planning Commiss, Minist Educ, Key Lab Neurosci, Beijing 100191, Peoples R China
7.Fujian Med Univ, Affiliated Mindong Hosp, Dept Orthopaed 8, Fuzhou 355000, Fujian, Peoples R China

Recommended Citation:
Dong, Shuying,Kong, Jian,Kong, Fandong,et al. Sorafenib suppresses the epithelial-mesenchymal transition of hepatocellular carcinoma cells after insufficient radiofrequency ablation[J]. BMC CANCER,2015,15.
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